Effect of Divergence in Patients’ Socioeconomic Background on their Perspective of the Role of the Community Pharmacist in Amman, Jordan

Purpose: To examine the effect of divergence in patients’ socioeconomic background on their perspective of the roles of the pharmacist in Amman, Jordan.Methods: This was a single-phase observational study conducted in two different socioeconomic areas: Western (WA) and Eastern Amman (EA, which is of a lower socioeconomic status than WA) in March to May of both 2009 and 2010. A validated questionnaire was completed by patients walking into community pharmacies. The source of advice (clinical specialist, general practitioner, pharmacist, nurse or herbalist) regarding patient's disease management and medication use, as well as patients’expectation of the pharmacist were investigated.Results: 2000 patients (mean age: 35.1 ± 13.7 years, 57.2 % males) visiting community pharmacies in Amman (1000 each from WA and EA) took part in the study. The majority of patients chose the pharmacist as the source of advice on medication use (WA, 50.8 %; EA, 53.6 %), followed by the clinical specialist (WA, 35.7 % vs EA, 26.9 %, p = 0.001). Other aspects of patient perspective and expectation of the pharmacist were also assessed; in several instances, more reliance on thepharmacist was observed in EA (lower socioeconomic status) than in WA.Conclusion: The findings of this study is important for future social pharmacy studies in the area, as it shows that socioeconomic status influences patient’s perception of the role of the community pharmacist in Amman, Jordan.Keywords: Socioeconomic status, Community pharmacist, Jordan, Patient perception, Counselin

Genotype and Haplotype Analysis of ABCB1 at 1236, 2677 and 3435 among Jordanian Population

Purpose: To determine the frequencies of important allelic variants and their haplotype frequencies of the gene among Jordanian population and to compare findings with those reported for other ethnic groups.Methods: Genotyping of ABCB1 (C1236T, G2677T/A and C3435T) was carried out on unrelated healthy Jordanian subjects. Different allelic variants were determined using polymerase chain reactionrestriction fragment length polymorphism (PCR-RFLP). The haplotype frequencies of these three SNPs were analyzed and compared them with those of other reported populations. Haplotype frequencies were calculated using Golden Helix Tree software and Linkage disequilibrium was represented by D”.Results: ABCB1 C3435T allele frequencies for C allele and T allele were 0.57 and 0.43, respectively. For ABCB1 G2677T/A the allele frequencies for G allele, T allele, and A allele were 0.65, 0.32 and 0.0, respectively. As for ABCB C1236T, its allele frequencies were 0.65 for C allele and 0.35 for T allele. C1236T, G2677T/A, and C3435T SNPs were expected to be structured in 8 different haplotypes with GC- C (37.6.0 %), T-T-T (18.6 %), G-C-T (14.3 %) and T-T-C (12 %) that were most prominent. The haplotype frequency distribution of our study group was found to be significantly different from those of Chinese, Indian, Japanese, African and Caucasian (p < 0.0001) and resemble Ashkenazi Jewish and Slovenian populations (p > 0.05).Conclusion: In addition to earlier studies, the findings of the current study provide evidence that suggest the use of genetic polymorphisms of ABCB1 SNPs as markers for ethnicity and ancestral origin. The analysis of haplotype and genotype can be useful in identifying the relation between ABCB1 polymorphism, disease susceptibility and drug disposition.Keywords: Genotype, Allele, MDR1, ABCB1, Polymorphism, Haplotype frequencie

Stroke Awareness in the General Population: A Study from Jordan

Purpose: To assess the awareness level of the Jordanian general population regarding the definition, risk factors, signs and symptoms, and consequences of stroke.Methods: This was a cross-sectional study. The questionnaire was handed to participants by trained students, the participants were chosen randomly from public places, and the questionnaire used is subdivided into demographic data, questions of multiple choices to identify symptoms and signs, the definition of stroke, and risk factors of stroke. The data were entered and analysed by SPSS software version 16.using frequencies, Chi square and logistic regression. A level of significance of 0.05 was applied.Results: At least 1993 questionnaires were returned. Out which 40 were excluded for poor quality and 99 were excluded due to missing data. From the 1854 questionnaire used, 82% were residents of Amman, and only 17% were older than 47 years of age. Females were almost equally represented as males,who accounted for 57% of the total. More than 70% had an educational level higher than secondary school.. Only 1.5% experienced a previous stroke, and a relatively high proportion (63.8%) knew someone who suffered from stroke. The most recognized symptom was speech loss (54.7%). All other symptoms were recognized by < 50% of the population. Seventy five percent knew that the brain is the organ involved in stroke while 85% would contact the ambulance on noticing stroke signs, even if symptoms subsequently improved. In a bivariate logistic regression, no independent variables had true effect on knowledge.Conclusion: We concluded from the study that there are serious gaps in knowledge regarding stroke; an educational program on stroke may help reduce the stroke burden in Jordan.Keywords: Stroke, Awareness, Jordan