Comparison of gene expression of metallothioneins, ubiquitin and p53 in fibroblasts from lung and skin of rats of different age

We studied gene expression of five metallothioneins (MT 1-5), ubiquitin and protein p53 and their products in fibroblasts culture of the skin and lungs of white rats of different ages (2 weeks, 1, 3, and 24 months) and determined its (metallothionein 1-5 types, ubiquitin, p53) product quantity. All these proteins are protective ones, but perform their functions by using different mechanisms. Metallothionein bind, transport and excrete ions of bivalent metals, ubiquitin controls the cleavage of the defective and short-lived proteins in the proteasome, protein p53 controls apoptosis, thus ensuring the genome stability. The similarity of age dynamics of gene expression of ubiquitin and MT of cells of both sources has been shown – maximum at 3 months. Expression of p53 gene has a difference: both in the skin and lungs expression increases up to 24 months. Product quantity of p53 has a minimum in the skin at 3 months and remains constant; in the lungs, this value has a maximum at 1 month

Protein alterations associated with temozolomide resistance in subclones of human glioblastoma cell lines

Temozolomide (TMZ) is the standard chemotherapeutic agent for human malignant glioma, but intrinsic or acquired chemoresistance represents a major obstacle to successful treatment of this highly lethal group of tumours. Obtaining better understanding of the molecular mechanisms underlying TMZ resistance in malignant glioma is important for the development of better treatment strategies. We have successfully established a passage control line (D54-C10) and resistant variants (D54-P5 and D54-P10) from the parental TMZ-sensitive malignant glioma cell line D54-C0. The resistant sub-cell lines showed alterations in cell morphology, enhanced cell adhesion, increased migration capacities, and cell cycle arrests. Proteomic analysis identified a set of proteins that showed gradual changes in expression according to their 50% inhibitory concentration (IC50). Successful validation was provided by transcript profiling in another malignant glioma cell line U87-MG and its resistant counterparts. Moreover, three of the identified proteins (vimentin, cathepsin D and prolyl 4-hydroxylase, beta polypeptide) were confirmed to be upregulated in high-grade glioma. Our data suggest that acquired TMZ resistance in human malignant glioma is associated with promotion of malignant phenotypes, and our reported molecular candidates may serve not only as markers of chemoresistance but also as potential therapeutic targets in the treatment of TMZ-resistant human malignant glioma, providing a platform for future investigations

The state and prospects of development of the market of Islamic investments

Banking and finances based on ethical standards of Muslims are now an integral part of international financial market. Vitality and stability of financial institutions in crisis period arose more and more interest to the in Russia and abroad. In article is presented analysis of condition and effectiveness of use of Islamic investment instrument, are detected major tendencies of this branch development. It was determined that financial instruments that correspond Islam principle have a big potential for development even in period of economic crises. But absence of special regulation slows process of their development. According to standards of Islamic ethic, righteous is only that wealth that is a fruit of own labor and entrepreneurial efforts, and also the one that is received as heritage or as a gift. Therefore, profit is considered as award for a risk associated with any business enterprise. Religious standards and taboos that are mandatory for conduction and adherence for every one Muslims, became a reason for genesis of Islamic investment funds (Bekkin R., 2015, p. 141). Increase of Islamic population size in developed countries promoted expansion of Islamic financial institutes; and, respectively, increased interest of investors from Islamic countries in geographical diversification of investments portfolios; increase of demands for socially responsible investment anticipating ranging of companies from point of view of their attentiveness to issues of environmental protection, improvement of labor conditions etc

The state and prospects of development of the market of Islamic investments

Banking and finances based on ethical standards of Muslims are now an integral part of international financial market. Vitality and stability of financial institutions in crisis period arose more and more interest to the in Russia and abroad. In article is presented analysis of condition and effectiveness of use of Islamic investment instrument, are detected major tendencies of this branch development. It was determined that financial instruments that correspond Islam principle have a big potential for development even in period of economic crises. But absence of special regulation slows process of their development. According to standards of Islamic ethic, righteous is only that wealth that is a fruit of own labor and entrepreneurial efforts, and also the one that is received as heritage or as a gift. Therefore, profit is considered as award for a risk associated with any business enterprise. Religious standards and taboos that are mandatory for conduction and adherence for every one Muslims, became a reason for genesis of Islamic investment funds (Bekkin R., 2015, p. 141). Increase of Islamic population size in developed countries promoted expansion of Islamic financial institutes; and, respectively, increased interest of investors from Islamic countries in geographical diversification of investments portfolios; increase of demands for socially responsible investment anticipating ranging of companies from point of view of their attentiveness to issues of environmental protection, improvement of labor conditions etc

La Patrie : journal quotidien, politique, commercial et littéraire

15 janvier 18691869/01/15 (A29)

PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila.

Lipid droplets are intracellular organelles enriched in adipose tissue that govern the body fat stores of animals. In mammals, members of the evolutionarily conserved PERILIPIN protein family are associated with the lipid droplet surface and participate in lipid homeostasis. Here, we show that Drosophila mutants lacking the PERILIPIN PLIN1 are hyperphagic and suffer from adult-onset obesity. PLIN1 is a central and Janus-faced component of fat metabolism. It provides barrier function to storage lipid breakdown and acts as a key factor of stimulated lipolysis by modulating the access of proteins to the lipid droplet surface. It also shapes lipid droplet structure, transforming unilocular into multilocular fat cells. We generated flies devoid of all PERILIPIN family members and show that they exhibit impaired yet functional body fat regulation. Our data reveal the existence of a basal and possibly ancient lipid homeostasis system