Observations by human subjects on radiation- induced light flashes in fast-neutron, X-ray, and positive-pion beams

Exposure of human subjects to fast neutron beam to determine cause of light flashes observed by astronauts on lunar mission

Human visual response to nuclear particle exposures

Experiments with accelerated helium ions were performed in an effort to localize the site of initial radiation interactions in the eye that lead to light flash observations by astronauts during spaceflight. The character and efficiency of helium ion induction of visual sensations depended on the state of dark adaptation of the retina; also, the same events were seen with different efficiencies and details when particle flux density changed. It was concluded that fast particles cause interactions in the retina, particularly in the receptor layer, and thus give rise to the sensations of light flashes, streaks, and supernovae

Observations of cosmic ray induced phosphenes

Phosphene observations by astronauts on flights near and far from earth atmosphere are discussed. It was concluded that phosphenes could be observed by the naked eye. Further investigation is proposed to determine realistic human tolerance levels for extended missions and to evaluate the need to provide special spacecraft shielding

Light flash phenomenon seen by astronauts

The results from experiments conducted to characterize and elucidate light flashes seen by astronauts on Apollo 11, 12, 13, and 14 during transluna or transearth orbit are presented. The data show cosmic nuclei interacting with the visual apparatus causes the light flash phenomenon. The data also suggest that slow protons and helium ions with a stopping power greater than 10 KeV/micron will cause light flashes and streaks in the partially dark adapted eye. The effects of galactic cosmic nuclei interacting with man during long term missions are discussed

The Qo site of the mitochondrial complex III is required for the transduction of hypoxic signaling via reactive oxygen species production

Mammalian cells increase transcription of genes for adaptation to hypoxia through the stabilization of hypoxia-inducible factor 1α (HIF-1α) protein. How cells transduce hypoxic signals to stabilize the HIF-1α protein remains unresolved. We demonstrate that cells deficient in the complex III subunit cytochrome b, which are respiratory incompetent, increase ROS levels and stabilize the HIF-1α protein during hypoxia. RNA interference of the complex III subunit Rieske iron sulfur protein in the cytochrome b–null cells and treatment of wild-type cells with stigmatellin abolished reactive oxygen species (ROS) generation at the Qo site of complex III. These interventions maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. Antioxidants maintained hydroxylation of HIF-1α protein and prevented stabilization of HIF-1α protein during hypoxia. Exogenous hydrogen peroxide under normoxia prevented hydroxylation of HIF-1α protein and stabilized HIF-1α protein. These results provide genetic and pharmacologic evidence that the Qo site of complex III is required for the transduction of hypoxic signal by releasing ROS to stabilize the HIF-1α protein

Direct Data Distribution From Low-Earth Orbit

NASA Lewis Research Center (LeRC) is developing the space and ground segment technologies necessary to demonstrate a direct data distribution (1)3) system for use in space-to-ground communication links from spacecraft in low-Earth orbit (LEO) to strategically located tracking ground terminals. The key space segment technologies include a K-band (19 GHz) MMIC-based transmit phased array antenna, and a multichannel bandwidth- and power-efficient digital encoder/modulate with an aggregate data rate of 622 Mb/s. Along with small (1.8 meter), low-cost tracking terminals on the ground, the D3 system enables affordable distribution of data to the end user or archive facility through interoperability with commercial terrestrial telecommunications networks. The D3 system is applicable to both government and commercial science and communications spacecraft in LEO. The features and benefits of the D3 system concept are described. Starting with typical orbital characteristics, a set of baseline requirements for representative applications is developed, including requirements for onboard storage and tracking terminals, and sample link budgets are presented. Characteristics of the transmit array antenna and digital encoder/modulator are described. The architecture and components of the tracking terminal are described, including technologies for the next generation terminal. Candidate flights of opportunity for risk mitigation and space demonstration of the D3 features are identified

Isolation and Comparative Transcriptome Analysis of Human Fetal and iPSC-Derived Cone Photoreceptor Cells.

Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9.pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies