A new DNA extraction method for automated food analysis

Effective DNA purification is essential for reliable composition and contamination analysis in the food industry. This paper compares two DNA extraction and purification procedures for food analysis. The commercially available DNA extraction kit from Agilent Technologies, Inc. was tested against a new solid phase extraction (SPE) method using silica beads embedded in a porous monolith structure that is integrated in a disposable column design. The technique is amenable to automation and streamlined workflow integration, with possible miniaturization of the sample handling process making it suitable for high-throughput applications. To demonstrate the automated process, the manual homogenizing step was compared to a custom automated procedure. To demonstrate the process for food testing, two types of sausages were analyzed for traces of porcine gDNA

The New Politics of the Welfare State? A Case Study of Extra-Parliamentary Party Politics in Norway

According to the literature on the ‘new politics of the welfare state’, party politics plays a minor role in welfare policy outputs today. In this article, we ask what the degree of politicisation is below the level of government. Focusing on two specific policy areas –pension reform and anti-poverty policy – and both substantive and procedural aspects of politicisation in the case of Norway, we identify party policies and map intra-party decision-making prior to the 2005 general election. We first conclude that neither policy area seemed to be strongly politicised, but nonetheless, there were limits to the ‘depoliticisation’ of welfare policy even in a consensual state like Norway. Hence, we show – or confirm – that counter-forces might exist between and within political parties in advanced industrial societies, yet to varying degrees across welfare policy fields.acceptedVersio

The agenda of British government: the speech from the throne, 1911-2008

This article considers how UK governments use the Speech from the Throne (also known as the Gracious Speech and the King's or the Queen's Speech) to define and articulate their executive and legislative agenda. The analysis uses the policy content coding system of the Policy Agendas Project to measure total executive and legislative attention to particular issues. This generates the longest known data series of the political agenda in the UK, from the date of the first Parliament Act in 1911 right up to the end of 2008, nearly a century of government agenda setting. Using these data, the article identifies long-run institutional and policy stability in this agenda-setting instrument, and variation in its length and executive–legislative content due to the focusing events of world wars and party control of government. It assesses the degree to which the policy content of the speech is persistent (autoregressive) over time and identifies long-term trends in the total number of topics mentioned in each speech (scope), and the dispersion of government attention across topics (entropy). It also identifies important variation over time that indicates change in the agenda-setting function of the speech and evolution of the agenda in response to policy challenges faced by modern British governments in the period since 1911. Overall, the analysis demonstrates the robustness of the speech as a measure of the policy agenda and executive priorities in the UK

Perspectives on marine zooplankton lipids

We developed new perspectives to identify important questions and to propose approaches for future research on marine food web lipids. They were related to (i) structure and function of lipids, (ii) lipid changes during critical life phases, (iii) trophic marker lipids, and (iv) potential impact of climate change. The first addresses the role of lipids in membranes, storage lipids, and buoyancy with the following key question: How are the properties of membranes and deposits affected by the various types of lipids? The second deals with the importance of various types of lipids during reproduction, development, and resting phases and addresses the role of the different storage lipids during growth and dormancy. The third relates to trophic marker lipids, which are an important tool to follow lipid and energy transfer through the food web. The central question is how can fatty acids be used to identify and quantify food web relationships? With the fourth, hypotheses are presented on effects of global warming, which may result in the reduction or change in abundance of large, lipid-rich copepods in polar oceans, thereby strongly affecting higher trophic levels. The key question is how will lipid dynamics respond to changes in ocean climate at high latitudes?