DESIGN AND STUDY OF DIMENSIONAL PARAMETERS INFLUENCE ON VORTEX TUBE BEHAVIOR

Vortex tube is a thermodynamic device, with no moving parts, applied to separate hot and cold air from compressed air injected into the tube. It has many applications in the industry, for example, among others, it can be mentioned electronic systems cooling, machining processes cooling and environmental chambers. This paper presents the design and tube dimensioning based on parameters and data found in the literature. Therefore, a prototype has been made and tested, which allowed the understanding of the influence of internal tube diameter and width on the hot and cold air temperatures while submitted to compressed air with pressure varying from 1 to 2.5bar. Results of tested configurations indicates that the relation between tube length and diameter (L/D) has small influence on vertex tube behavior, meanwhile, 3/8” tube diameter shows lowest temperatures on cold flow (-6.5°C, -8.0°C and -8.5°C) and higher COP (≈ 0.15)

Centrifuge pellet injector for JET

SIGLEDEGerman

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody

Funding: This work was supported by PRF 2016-67, Progetti di Ricerca, Sapienza University of Rome (RP1181642E87148C), AIRC IG-24614 to IS, FIRC (22392) to MLT and IS, CIB (http://www.cibiotech.it/) and Fondazione Buzzati Traverso (813 - https://www.fondazioneadrianobuzzatitraverso.it) to MLT and IS; Avvio alla Ricerca, Sapienza University of Rome (AR2181642B6F2E48, AR1181642EE61111) to RB, SDG and IS. CM has been supported by EMBO ST fellowship 7621, Veronesi TG 2019. MLT is supported by Be For ERC, Sapienza. IC is supported by R01GM117376 and NSF Career 1751197. JGC is supported by a Wellcome Trust Senior Research Fellowship 206346/Z/17/Z. This work was supported in part by the Francis Crick Institute which receives its core funding from Cancer Research UK (FC001002), the UK Medical Research Council (FC001002), and the Wellcome Trust (FC001002). For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.To complete mitosis, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by the endosomal sorting complex required for transport (ESCRT) machinery. AKTIP, a protein discovered to be associated with telomeres and the nuclear membrane in interphase cells, shares sequence similarities with the ESCRT I component TSG101. Here we present evidence that during mitosis AKTIP is part of the ESCRT machinery at the midbody. AKTIP interacts with the ESCRT I subunit VPS28 and forms a circular supra-structure at the midbody, in close proximity with TSG101 and VPS28 and adjacent to the members of the ESCRT III module CHMP2A, CHMP4B and IST1. Mechanistically, the recruitment of AKTIP is dependent on MKLP1 and independent of CEP55. AKTIP and TSG101 are needed together for the recruitment of the ESCRT III subunit CHMP4B and in parallel for the recruitment of IST1. Alone, the reduction of AKTIP impinges on IST1 and causes multinucleation. Our data altogether reveal that AKTIP is a component of the ESCRT I module and functions in the recruitment of ESCRT III components required for abscission.Depto. de Biología CelularFac. de Ciencias BiológicasTRUEpu