12 research outputs found
Bioenergetic status and hypoxia in Lewis lung carcinoma assessed by 31P NMR spectroscopy: correlation with tumor progression
Aim: Π’o evaluate the hypoxia level and some indices of Lewis lung carcinoma energy metabolism by means of 31Π NMR spectroscopy in perchloric acid (PCA) tissue extracts during growth of primary tumor and metastasis. Πaterials and Methods: C57Bl/6 mice-bearing Lewis lung carcinoma were used in this study. Tumor energy metabolism was studied by 31P NMR spectroscopy and the metabolic NMR ratios were used as parameters for metabolic status and hypoxia level. Results: It was shown that growth of primary tumor is accompanied with increase of Pi/PCr, Pi/bNΠ’P and PME/bNΠ’P ratios that reflect drop of tumor energy status and oxygenation level in tumor tissue. These changes in relevant metabolic ratios correlate with enlargement of primary tumor volume (r = 0.87, p = 0.0045; r = 0.90, p = 0.0012; r = 0.764, p = 0.05, respectively) as well as with the number of lung metastases (rs = 0.761, p = 0.028; rs = 0.86, p = 0.0049; rs = 0.77, p = 0.040, respectively). Conclusion: In present study it was shown that 31Π NMR spectroscopy of PCA tumor tissue extracts may be used as reliable method for the assessment of the level of oxygenation as well as changes in energy metabolism in the experimental tumors. It may be helpful to evaluate the energy status of human tumors by investigation both of biopsy and surgical specimens. Hypoxia and hypoxia-associated metabolic events in primary tumor are linked with malignant progression, in particular metastasis.Π¦Π΅Π»Ρ: ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΡΠΎΠ²Π΅Π½Ρ Π³ΠΈΠΏΠΎΠΊΡΠΈΠΈ ΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΠΈ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ Π»Π΅Π³ΠΊΠΎΠ³ΠΎ ΠΡΡΠΈΡ
Ρ ΠΏΠΎΠΌΠΎΡΡΡ 31Π Π―ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΠΈ ΠΏΠ΅ΡΡ
Π»ΠΎΡΠ½ΡΡ
(Π Π‘Π) ΡΠΊΡΡΡΠ°ΠΊΡΠΎΠ² ΡΠΊΠ°Π½ΠΈ Π² ΠΏΡΠΎΡΠ΅ΡΡΠ΅ ΡΠΎΡΡΠ° ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ Π΅Π΅
ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ. ΠaΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π² ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΌΡΡΠ΅ΠΉ C57Bl/6 Ρ ΠΏΠ΅ΡΠ΅Π²ΠΈΠ²Π½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΠΎΠΉ Π»Π΅Π³ΠΊΠΎΠ³ΠΎ
ΠΡΡΠΈΡ. ΠΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈΠ·ΡΡΠ°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ Π―ΠΠ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΠΈ, oΡΠ½ΠΎΡΠ΅Π½ΠΈΡ ΡΠΎΡΡΠΎΡΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠΎΠ² ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° ΠΈ ΡΡΠΎΠ²Π½Ρ Π³ΠΈΠΏΠΎΠΊΡΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ,
ΡΡΠΎ ΡΠΎΡΡ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°Π΅ΡΡΡ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΉ Pi/PCr, Pi/Ξ²NΠ’P and PME/Ξ²NΠ’P Π² ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ, ΡΡΠΎ
ΠΎΡΡΠ°ΠΆΠ°Π΅Ρ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΡΠΎΠ²Π½Ρ Π±ΠΈΠΎΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° ΠΈ ΡΡΠΎΠ²Π½Ρ ΠΎΠΊΡΠΈΠ³Π΅Π½Π°ΡΠΈΠΈ Π² ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ. ΠΡΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΉ
Π²Π°ΠΆΠ½ΡΡ
ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΊΠΎΡΡΠ΅Π»ΠΈΡΡΡΡ Ρ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΎΠ±ΡΠ΅ΠΌΠ° ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ (r = 0,87, p = 0,0045; r =
0,90, p = 0,0012; r = 0,764, p = 0,05 ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ) ΠΈ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²ΠΎΠΌ ΠΌΠ΅ΡΠ°ΡΠ°Π·ΠΎΠ² Π² Π»Π΅Π³ΠΊΠΈΡ
(rs
= 0,761, p = 0,028; rs
= 0,86,
p = 0,0049; rs
= 0,77, p = 0,040 ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ). ΠΡΠ²ΠΎΠ΄Ρ: ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ, ΡΡΠΎ 31Π Π―ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΡ PCA ΡΠΊΠ°Π½Π΅Π²ΡΡ
ΡΠΊΡΡΡΠ°ΠΊΡΠΎΠ² ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ Π½Π°Π΄Π΅ΠΆΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΎΡΠ΅Π½ΠΊΠΈ ΡΡΠΎΠ²Π½Ρ ΠΎΠΊΡΠΈΠ³Π΅Π½Π°ΡΠΈΠΈ ΠΈ Π±ΠΈΠΎΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ°ΡΡΡΠ° ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ. ΠΡΠΎΡ ΠΌΠ΅ΡΠΎΠ΄ ΠΌΠΎΠΆΠ΅Ρ Π±ΡΡΡ ΠΏΠΎΠ»Π΅Π·Π½ΡΠΌ ΠΏΡΠΈ ΠΎΡΠ΅Π½ΠΊΠ΅ ΡΠ½Π΅ΡΠ³Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ° ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΡΠ΅Π»ΠΎΠ²Π΅ΠΊΠ°, Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ ΠΏΡΠΈ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ Π±ΠΈΠΎΠΏΡΠΈΠΉΠ½ΠΎΠ³ΠΎ ΠΈ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π°. ΠΠΈΠΏΠΎΠΊΡΠΈΡ ΠΈ Π³ΠΈΠΏΠΎΠΊΡΠΈΡ-Π°ΡΡΠΎΡΠΈΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΌΠ΅ΡΠ°Π±ΠΎΠ»ΠΈΠ·ΠΌΠ°
Π² ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΡΠ²ΡΠ·Π°Π½Ρ ΡΠΎ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΠ΅ΠΉ, Π² ΡΠ°ΡΡΠ½ΠΎΡΡΠΈ Ρ ΠΌΠ΅ΡΠ°ΡΡΠ°Π·ΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ
An arsenal of magnetic nanoparticles; Perspectives in the treatment of cancer
Nanomedicine is an emerging field, which constitutes a new direction in the treatment of cancer. Magnetic nanoparticles (MNPs) can circumvent vascular tissue to concentrate at the site of the tumor. Under the influence of an external, alternating magnetic field, MNPs generate high temperatures within the tumor and ablate malignant cells while inflicting minimal damage to healthy host tissue. Due to their theranostic properties, they constitute a promising candidate for the treatment of cancer. A critical review of the type, size and therapeutic effect of different MNPs is presented, following an appraisal of the literature in the last 5 years. This is a multibillion dollar industry, with a few studies moving to clinical trials within the next 5 years