Use of ascorbic and thioglycollic acids to eliminate interference from iron in the aluminon method for determining aluminium

The use of ascorbic and thioglycollic acids as inhibitors for the interference of iron in the aluminon method of Hsu have been examined. The use of ascorbic acid, as proposed by Jayman and Sivasubramaniam, has been found to change iron interference from positive to negative causing aluminium to be underestimated. However, the addition of 0.2 ml of a 1% V/V solution of thioglycollic acid to solutions containing aluminium in amounts ranging from 10 to 50 μg has been proved to suppress the interference from up to 900 μg of iron.Peer Reviewe

High Conservation in Transcriptomic and Proteomic Response of White Sturgeon to Equipotent Concentrations of 2,3,7,8-TCDD, PCB 77, and Benzo[a]pyrene

Adverse effects associated with exposure to dioxin-like compounds (DLCs) are mediated primarily through activation of the aryl hydrocarbon receptor (AHR). However, little is known about the cascades of events that link activation of the AHR to apical adverse effects. Therefore, this study used high-throughput, next-generation molecular tools to investigate similarities and differences in whole transcriptome and whole proteome responses to equipotent concentrations of three agonists of the AHR, 2,3,7,8-TCDD, PCB 77, and benzo­[a]­pyrene, in livers of a nonmodel fish, the white sturgeon (<i>Acipenser transmontanus</i>). A total of 926 and 658 unique transcripts were up- and down-regulated, respectively, by one or more of the three chemicals. Of the transcripts shared by responses to all three chemicals, 85% of up-regulated transcripts and 75% of down-regulated transcripts had the same magnitude of response. A total of 290 and 110 unique proteins were up- and down-regulated, respectively, by one or more of the three chemicals. Of the proteins shared by responses to all three chemicals, 70% of up-regulated proteins and 48% of down-regulated proteins had the same magnitude of response. Among treatments there was 68% similarity between the global transcriptome and global proteome. Pathway analysis revealed that perturbed physiological processes were indistinguishable between equipotent concentrations of the three chemicals. The results of this study contribute toward more completely describing adverse outcome pathways associated with activation of the AHR