1,286 research outputs found
BLUF Domain Function Does Not Require a Metastable Radical Intermediate State
BLUF
(blue light using flavin) domain proteins are an important
family of blue light-sensing proteins which control a wide variety
of functions in cells. The primary light-activated step in the BLUF
domain is not yet established. A number of experimental and theoretical
studies points to a role for photoinduced electron transfer (PET)
between a highly conserved tyrosine and the flavin chromophore to
form a radical intermediate state. Here we investigate the role of
PET in three different BLUF proteins, using ultrafast broadband transient
infrared spectroscopy. We characterize and identify infrared active
marker modes for excited and ground state species and use them to
record photochemical dynamics in the proteins. We also generate mutants
which unambiguously show PET and, through isotope labeling of the
protein and the chromophore, are able to assign modes characteristic
of both flavin and protein radical states. We find that these radical
intermediates are not observed in two of the three BLUF domains studied,
casting doubt on the importance of the formation of a population of
radical intermediates in the BLUF photocycle. Further, unnatural amino
acid mutagenesis is used to replace the conserved tyrosine with fluorotyrosines,
thus modifying the driving force for the proposed electron transfer
reaction; the rate changes observed are also not consistent with a
PET mechanism. Thus, while intermediates of PET reactions can be observed
in BLUF proteins they are not correlated with photoactivity, suggesting
that radical intermediates are not central to their operation. Alternative
nonradical pathways including a keto–enol tautomerization induced
by electronic excitation of the flavin ring are considered
Ultrafast Structural Dynamics of BlsA, a Photoreceptor from the Pathogenic Bacterium Acinetobacter baumannii
Acinetobacter baumannii is an important human pathogen that can form biofilms and persist under harsh environmental conditions. Biofilm formation and virulence are modulated by blue light, which is thought to be regulated by a BLUF protein, BlsA. To understand the molecular mechanism of light sensing, we have used steady-state and ultrafast vibrational spectroscopy to compare the photoactivation mechanism of BlsA to the BLUF photosensor AppA from Rhodobacter sphaeroides. Although similar photocycles are observed, vibrational data together with homology modeling identify significant differences in the β5 strand in BlsA caused by photoactivation, which are proposed to be directly linked to downstream signaling
Stimulation of paralysed quadriceps muscles with sequentially and spatially distributed electrodes during dynamic knee extension
Background: During functional electrical stimulation (FES) tasks with able-bodied (AB) participants, spatially distributed sequential stimulation (SDSS) has demonstrated substantial improvements in power output and fatigue properties compared to conventional single electrode stimulation (SES). The aim of this study was to compare the properties of SDSS and SES in participants with spinal cord injury (SCI) in a dynamic isokinetic knee extension task simulating knee movement during recumbent cycling.
Method: Using a case-series design, m. vastus lateralis and medialis of four participants with motor and sensory complete SCI (AIS A) were stimulated for 6 min on both legs with both electrode setups. With SES, target muscles were stimulated by a pair of electrodes. In SDSS, the distal electrodes were replaced by four small electrodes giving the same overall stimulation frequency and having the same total surface area. Torque was measured during knee extension by a dynamometer at an angular velocity of 110 deg/s. Mean power of the left and right sides (PmeanL,R) was calculated from all stimulated extensions for initial, final and all extensions. Fatigue is presented as an index value with respect to initial power from 1 to 0, whereby 1 means no fatigue.
Results: SDSS showed higher PmeanL,R values for all four participants for all extensions (increases of 132% in participant P1, 100% in P2, 36% in P3 and 18% in P4 compared to SES) and for the initial phase (increases of 84%, 59%, 66%, and 16%, respectively). Fatigue resistance was better with SDSS for P1, P2 and P4 but worse for P3 (0.47 vs 0.35, 0.63 vs 0.49, 0.90 vs 0.82 and 0.59 vs 0.77, respectively).
Conclusion: Consistently higher PmeanL,R was observed for all four participants for initial and overall contractions using SDSS. This supports findings from previous studies with AB participants. Fatigue properties were better in three of the four participants. The lower fatigue resistance with SDSS in one participant may be explained by a very low muscle activation level in this case. Further investigation in a larger cohort is warranted
Decoupling heavy sparticles in Effective SUSY scenarios: Unification, Higgs masses and tachyon bounds
Using two-loop renormalization group equations implementing the decoupling of
heavy scalars, Effective SUSY scenarios are studied in the limit in which there
is a single low energy Higgs field. Gauge coupling unification is shown to hold
with similar or better precision than in standard MSSM scenarios. b-tau
unification is examined, and Higgs masses are computed using the effective
potential, including two-loop contributions from scalars. A 125 GeV Higgs is
compatible with stops/sbottoms at around 300 GeV with non-universal boundary
conditions at the scale of the heavy sparticles if some of the trilinear
couplings at this scale take values of the order of 1-2 TeV; if more
constrained boundary conditions inspired by msugra or gauge mediation are set
at a higher scale, heavier colored sparticles are required in general. Finally,
since the decoupled RG flow for third-generation scalar masses departs very
significantly from the MSSM DR-bar one, tachyon bounds for light scalars are
revisited and shown to be relaxed by up to a TeV or more.Comment: 35 pages, 17 figures. v2: Updated some scans, allowing for changes in
sign of some parameters, minor improvements. v3: Typos corrected in formulae
in the appendices, added some clarifying remarks about flavor mixing being
ignore
Excess Higgs Production in Neutralino Decays
The ATLAS and CMS experiments have recently claimed discovery of a Higgs
boson-like particle at ~5 sigma confidence and are beginning to test the
Standard Model predictions for its production and decay. In a variety of
supersymmetric models, a neutralino NLSP can decay dominantly to the Higgs and
the LSP. In natural SUSY models, a light third generation squark decaying
through this chain can lead to large excess Higgs production while evading
existing BSM searches. Such models can be observed at the 8 TeV LHC in channels
exploiting the rare diphoton decays of the Higgs produced in the cascade decay.
Identifying a diphoton resonance in association with missing energy, a lepton,
or b-tagged jets is a promising search strategy for discovery of these models,
and would immediately signal new physics involving production of a Higgs boson.
We also discuss the possibility that excess Higgs production in these SUSY
decays can be responsible for enhancements of up to 50% over the SM prediction
for the observed rate in the existing inclusive diphoton searches, a scenario
which would likely by the end of the 8 TeV run be accompanied by excesses in
the diphoton + lepton/MET and SUSY multi-lepton/b searches and a potential
discovery in a diphoton + 2b search.Comment: 42 pages, 19 figure
Infarction in the territory of the anterior cerebral artery: clinical study of 51 patients
<p>Abstract</p> <p>Background</p> <p>Little is known about clinical features and prognosis of patients with ischaemic stroke caused by infarction in the territory of the anterior cerebral artery (ACA). This single centre, retrospective study was conducted with the following objectives: a) to describe the clinical characteristics and short-term outcome of stroke patients with ACA infarction as compared with that of patients with ischaemic stroke due to middle cerebral artery (MCA) and posterior cerebral artery (PCA) infarctions, and b) to identify predictors of ACA stroke.</p> <p>Methods</p> <p>Fifty-one patients with ACA stroke were included in the "Sagrat Cor Hospital of Barcelona Stroke Registry" during a period of 19 years (1986–2004). Data from stroke patients are entered in the stroke registry following a standardized protocol with 161 items regarding demographics, risk factors, clinical features, laboratory and neuroimaging data, complications and outcome. The characteristics of these 51 patients with ACA stroke were compared with those of the 1355 patients with MCA infarctions and 232 patients with PCA infarctions included in the registry.</p> <p>Results</p> <p>Infarctions of the ACA accounted for 1.3% of all cases of stroke (<it>n </it>= 3808) and 1.8% of cerebral infarctions (<it>n </it>= 2704). Stroke subtypes included cardioembolic infarction in 45.1% of patients, atherothrombotic infarction in 29.4%, lacunar infarct in 11.8%, infarct of unknown cause in 11.8% and infarction of unusual aetiology in 2%. In-hospital mortality was 7.8% (<it>n </it>= 4). Only 5 (9.8%) patients were symptom-free at hospital discharge. Speech disturbances (odds ratio [OR] = 0.48) and altered consciousness (OR = 0.31) were independent variables of ACA stroke in comparison with MCA infarction, whereas limb weakness (OR = 9.11), cardioembolism as stroke mechanism (OR = 2.49) and sensory deficit (OR = 0.35) were independent variables associated with ACA stroke in comparison with PCA infarction.</p> <p>Conclusion</p> <p>Cardioembolism is the main cause of brain infarction in the territory of the ACA. Several clinical features are more frequent in stroke patients with ACA infarction than in patients with ischaemic stroke due to infarction in the MCA and PCA territories.</p
Calculations of collisions between cold alkaline earth atoms in a weak laser field
We calculate the light-induced collisional loss of laser-cooled and trapped
magnesium atoms for detunings up to 50 atomic linewidths to the red of the
^1S_0-^1P_1 cooling transition. We evaluate loss rate coefficients due to both
radiative and nonradiative state-changing mechanisms for temperatures at and
below the Doppler cooling temperature. We solve the Schrodinger equation with a
complex potential to represent spontaneous decay, but also give analytic models
for various limits. Vibrational structure due to molecular photoassociation is
present in the trap loss spectrum. Relatively broad structure due to absorption
to the Mg_2 ^1Sigma_u state occurs for detunings larger than about 10 atomic
linewidths. Much sharper structure, especially evident at low temperature,
occurs even at smaller detunings due to of Mg_2 ^1Pi_g absorption, which is
weakly allowed due to relativistic retardation corrections to the forbidden
dipole transition strength. We also perform model studies for the other
alkaline earth species Ca, Sr, and Ba and for Yb, and find similar qualitative
behavior as for Mg.Comment: 20 pages, RevTex, 13 eps figures embedde
Constraints on supersymmetry with light third family from LHC data
We present a re-interpretation of the recent ATLAS limits on supersymmetry in
channels with jets (with and without b-tags) and missing energy, in the context
of light third family squarks, while the first two squark families are
inaccessible at the 7 TeV run of the Large Hadron Collider (LHC). In contrast
to interpretations in terms of the high-scale based constrained minimal
supersymmetric standard model (CMSSM), we primarily use the low-scale
parametrisation of the phenomenological MSSM (pMSSM), and translate the limits
in terms of physical masses of the third family squarks. Side by side, we also
investigate the limits in terms of high-scale scalar non-universality, both
with and without low-mass sleptons. Our conclusion is that the limits based on
0-lepton channels are not altered by the mass-scale of sleptons, and can be
considered more or less model-independent.Comment: 20 pages, 8 figures, 2 tables. Version published in JHE
Top quark forward-backward asymmetry in R-parity violating supersymmetry
The interaction of bottom squark-mediated top quark pair production,
occurring in the R-parity violating minimal supersymmetric standard model
(MSSM), is proposed as an explanation of the anomalously large
forward-backward asymmetry (FBA) observed at the Tevatron. We find that this
model can give a good fit to top quark data, both the inclusive and invariant
mass-dependent asymmetries, while remaining consistent (at the 2-
level) with the total and differential production cross-sections. The scenario
is challenged by strong constraints from atomic parity violation (APV), but we
point out an extra diagram for the effective down quark-Z vertex, involving the
same coupling constant as required for the FBA, which tends to weaken the APV
constraint, and which can nullify it for reasonable values of the top squark
masses and mixing angle. Large contributions to flavor-changing neutral
currents can be avoided if only the third generation of sparticles is light.Comment: 24 pages, 7 figures. v3: included LHC top production cross section
data; model still consistent at 2 sigma leve
Constraining compressed supersymmetry using leptonic signatures
We study the impact of the multi-lepton searches at the LHC on supersymmetric
models with compressed mass spectra. For such models the acceptances of the
usual search strategies are significantly reduced due to requirement of large
effective mass and missing E_T. On the other hand, lepton searches do have much
lower thresholds for missing E_T and p_T of the final state objects. Therefore,
if a model with a compressed mass spectrum allows for multi-lepton final
states, one could derive constraints using multi-lepton searches. For a class
of simplified models we study the exclusion limits using ATLAS multi-lepton
search analyses for the final states containing 2-4 electrons or muons with a
total integrated luminosity of 1-2/fb at \sqrt{s}=7 TeV. We also modify those
analyses by imposing additional cuts, so that their sensitivity to compressed
supersymmetric models increase. Using the original and modified analyses, we
show that the exclusion limits can be competitive with jet plus missing E_T
searches, providing exclusion limits up to gluino masses of 1 TeV. We also
analyse the efficiencies for several classes of events coming from different
intermediate state particles. This allows us to assess exclusion limits in
similar class of models with different cross sections and branching ratios
without requiring a Monte Carlo simulation.Comment: 18 pages, 5 figure
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