19 research outputs found
Avaliação de desempenho: discutindo a tecnologia para o planejamento e gestão de recursos humanos em saúde
Eixos de desenvolvimento econômico e geração de conflitos socioambientais no Brasil: desafios para a sustentabilidade e a justiça ambiental
Cognitive Dysfunction Is Sustained after Rescue Therapy in Experimental Cerebral Malaria, and Is Reduced by Additive Antioxidant Therapy
Neurological impairments are frequently detected in children surviving cerebral malaria (CM), the most severe neurological complication of infection with Plasmodium falciparum. The pathophysiology and therapy of long lasting cognitive deficits in malaria patients after treatment of the parasitic disease is a critical area of investigation. In the present study we used several models of experimental malaria with differential features to investigate persistent cognitive damage after rescue treatment. Infection of C57BL/6 and Swiss (SW) mice with Plasmodium berghei ANKA (PbA) or a lethal strain of Plasmodium yoelii XL (PyXL), respectively, resulted in documented CM and sustained persistent cognitive damage detected by a battery of behavioral tests after cure of the acute parasitic disease with chloroquine therapy. Strikingly, cognitive impairment was still present 30 days after the initial infection. In contrast, BALB/c mice infected with PbA, C57BL6 infected with Plasmodium chabaudi chabaudi and SW infected with non lethal Plasmodium yoelii NXL (PyNXL) did not develop signs of CM, were cured of the acute parasitic infection by chloroquine, and showed no persistent cognitive impairment. Reactive oxygen species have been reported to mediate neurological injury in CM. Increased production of malondialdehyde (MDA) and conjugated dienes was detected in the brains of PbA-infected C57BL/6 mice with CM, indicating high oxidative stress. Treatment of PbA-infected C57BL/6 mice with additive antioxidants together with chloroquine at the first signs of CM prevented the development of persistent cognitive damage. These studies provide new insights into the natural history of cognitive dysfunction after rescue therapy for CM that may have clinical relevance, and may also be relevant to cerebral sequelae of sepsis and other disorders
In vitro co-cultures of Pinus pinaster with Bursaphelenchus xylophilus: a biotechnological approach to study pine wilt disease
Abstract
Main conclusion Co-cultures of Pinus pinaster with
Bursaphelenchus xylophilus were established as a
biotechnological tool to evaluate the effect of nematotoxics
addition in a host/parasite culture system.
The pinewood nematode (PWN), Bursaphelenchus xylophilus,
the causal agent of pine wilt disease (PWD), was
detected for the first time in Europe in 1999 spreading
throughout the pine forests in Portugal and recently in
Spain. Plant in vitro cultures may be a useful experimental
system to investigate the plant/nematode relationships in
loco, thus avoiding the difficulties of field assays. In this
study, Pinus pinaster in vitro cultures were established and
compared to in vivo 1 year-old plantlets by analyzing shoot
structure and volatiles production. In vitro co-cultures were
established with the PWN and the effect of the phytoparasite
on in vitro shoot structure, water content and
volatiles production was evaluated. In vitro shoots showed
similar structure and volatiles production to in vivo maritime
pine plantlets. The first macroscopic symptoms of
PWD were observed about 4 weeks after in vitro co-culture
establishment. Nematode population in the culture medium
increased and PWNs were detected in gaps of the callus
tissue and in cavities developed from the degradation of
cambial cells. In terms of volatiles main components,
plantlets, P. pinaster cultures, and P. pinaster with B. xylophilus
co-cultures were all b- and a-pinene rich. Cocultures
may be an easy-to-handle biotechnological approach
to study this pathology, envisioning the understanding
of and finding ways to restrain this highly
devastating nematode.
Keywords Maritime pine ! Monoxenic culture !
Pinewood nematode ! Relative water content ! Shoots
structure ! Volatiles
Abbreviations
BAP 6-Benzylaminopurine
DAI Days after inoculation
EPPO European and Mediterranean Plant Protectio