209 research outputs found
The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken
The replacement histone H2A.Z is variously reported
as being linked to gene expression and preventing the
spread of heterochromatin in yeast, or concentrated
at heterochromatin in mammals. To resolve this
apparent dichotomy, affinity-purified antibodies
against the N-terminal region of H2A.Z, in both a triacetylatedandnon-
acetylatedstate, areusedin native
chromatin immmuno-precipitation experiments with
mononucleosomes from three chicken cell types. The
hyperacetylated species concentrates at the 50 end of
active genes, both tissue specific and housekeeping
but is absent from inactive genes, while the
unacetylated form is absent from both active and
inactive genes. A concentration of H2A.Z is also
found at insulators under circumstances implying a
link to barrier activity but not to enhancer blocking.
Although acetylated H2A.Z is widespread throughout
the interphase genome, at mitosis its acetylation is
erased, the unmodified form remaining. Thus,
although H2A.Z may operate as an epigenetic marker
for active genes, its N-terminal acetylation does not
The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken
The replacement histone H2A.Z is variously reported
as being linked to gene expression and preventing the
spread of heterochromatin in yeast, or concentrated
at heterochromatin in mammals. To resolve this
apparent dichotomy, affinity-purified antibodies
against the N-terminal region of H2A.Z, in both a triacetylatedandnon-
acetylatedstate, areusedin native
chromatin immmuno-precipitation experiments with
mononucleosomes from three chicken cell types. The
hyperacetylated species concentrates at the 50 end of
active genes, both tissue specific and housekeeping
but is absent from inactive genes, while the
unacetylated form is absent from both active and
inactive genes. A concentration of H2A.Z is also
found at insulators under circumstances implying a
link to barrier activity but not to enhancer blocking.
Although acetylated H2A.Z is widespread throughout
the interphase genome, at mitosis its acetylation is
erased, the unmodified form remaining. Thus,
although H2A.Z may operate as an epigenetic marker
for active genes, its N-terminal acetylation does not
Understanding person acquisition using an interactive activation and competition network
Face perception is one of the most developed visual skills that humans display, and recent work has attempted to examine the mechanisms involved in face perception through noting how neural networks achieve the same performance. The purpose of the present paper is to extend this approach to look not just at human face recognition, but also at human face acquisition. Experiment 1 presents empirical data to describe the acquisition over time of appropriate representations for newly encountered faces. These results are compared with those of Simulation 1, in which a modified IAC network capable of modelling the acquisition process is generated. Experiment 2 and Simulation 2 explore the mechanisms of learning further, and it is demonstrated that the acquisition of a set of associated new facts is easier than the acquisition of individual facts in isolation of one another. This is explained in terms of the advantage gained from additional inputs and mutual reinforcement of developing links within an interactive neural network system. <br/
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Dimensional errors in LIGA-produced metal structures due to thermal expansion and swelling of PMMA.
Numerical methods are used to examine dimensional errors in metal structures microfabricated by the LIGA process. These errors result from elastic displacements of the PMMA mold during electrodeposition and arise from thermal expansion of the PMMA when electroforming is performed at elevated temperatures and from PMMA swelling due to absorption of water from aqueous electrolytes. Both numerical solutions and simple analytical approximations describing PMMA displacements for idealized linear and axisymmetric geometries are presented and discussed. We find that such displacements result in tapered metal structures having sidewall slopes up to 14 {micro}m per millimeter of height for linear structures bounded by large areas of PMMA. Tapers for curved structures are of similar magnitude, but these structures are additionally skewed from the vertical. Potential remedies for reducing dimensional errors are also discussed. Here we find that auxiliary moat-like features patterned into the PMMA surrounding mold cavities can reduce taper by an order of magnitude or more. Such moats dramatically reduce tapers for all structures, but increase skew for curved structures when the radius of curvature is comparable to the structure height
The microscopic spin-phonon coupling constants in CuGeO_3
Using RPA results, mean field theory, and refined data for the polarization
vectors we determine the coupling constants of the four Peierls-active phonon
modes to the spin chains of CuGeO_3. We then derive the values of the coupling
of the spin system to the linear ionic displacements, the bond lengths and the
angles between bonds. Our values are consistent with microscopic theories and
various experimental results. We discuss the applicability of static approaches
to the spin-phonon coupling. The c-axis anomaly of the thermal expansion is
explained. We give the values of the coupling constants in an effective
one-dimensional Hamiltonian.Comment: 11 pages, two figures, 13 tables, PRB 59 (in press
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Epstein-Barr virus: clinical and epidemiological revisits and genetic basis of oncogenesis
Epstein-Barr virus (EBV) is classified as a member in the order herpesvirales, family herpesviridae, subfamily gammaherpesvirinae and the genus lymphocytovirus. The virus is an exclusively human pathogen and thus also termed as human herpesvirus 4 (HHV4). It was the first oncogenic virus recognized and has been incriminated in the causation of tumors of both lymphatic and epithelial nature. It was reported in some previous studies that 95% of the population worldwide are serologically positive to the virus. Clinically, EBV primary infection is almost silent, persisting as a life-long asymptomatic latent infection in B cells although it may be responsible for a transient clinical syndrome called infectious mononucleosis. Following reactivation of the virus from latency due to immunocompromised status, EBV was found to be associated with several tumors. EBV linked to oncogenesis as detected in lymphoid tumors such as Burkitt's lymphoma (BL), Hodgkin's disease (HD), post-transplant lymphoproliferative disorders (PTLD) and T-cell lymphomas (e.g. Peripheral T-cell lymphomas; PTCL and Anaplastic large cell lymphomas; ALCL). It is also linked to epithelial tumors such as nasopharyngeal carcinoma (NPC), gastric carcinomas and oral hairy leukoplakia (OHL). In vitro, EBV many studies have demonstrated its ability to transform B cells into lymphoblastoid cell lines (LCLs). Despite these malignancies showing different clinical and epidemiological patterns when studied, genetic studies have suggested that these EBV- associated transformations were characterized generally by low level of virus gene expression with only the latent virus proteins (LVPs) upregulated in both tumors and LCLs. In this review, we summarize some clinical and epidemiological features of EBV- associated tumors. We also discuss how EBV latent genes may lead to oncogenesis in the different clinical malignancie
Novel genetic loci associated with hippocampal volume
The hippocampal formation is a brain structure integrally involved in episodic memory, spatial navigation, cognition and stress responsiveness. Structural abnormalities in hippocampal volume and shape are found in several common neuropsychiatric disorders. To identify the genetic underpinnings of hippocampal structure here we perform a genome-wide association study (GWAS) of 33,536 individuals and discover six independent loci significantly associated with hippocampal volume, four of them novel. Of the novel loci, three lie within genes (ASTN2, DPP4 and MAST4) and one is found 200 kb upstream of SHH. A hippocampal subfield analysis shows that a locus within the MSRB3 gene shows evidence of a localized effect along the dentate gyrus, subiculum, CA1 and fissure. Further, we show that genetic variants associated with decreased hippocampal volume are also associated with increased risk for Alzheimer's disease (rg =-0.155). Our findings suggest novel biological pathways through which human genetic variation influences hippocampal volume and risk for neuropsychiatric illness
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