Novel polyomaviruses in mammals from multiple orders and reassessment of polyomavirus evolution and taxonomy

As the phylogenetic organization of mammalian polyomaviruses is complex and currently incompletely resolved, we aimed at a deeper insight into their evolution by identifying polyomaviruses in host orders and families that have either rarely or not been studied. Sixteen unknown and two known polyomaviruses were identified in animals that belong to 5 orders, 16 genera, and 16 species. From 11 novel polyomaviruses, full genomes could be determined. Splice sites were predicted for large and small T antigen (LTAg, STAg) coding sequences (CDS) and examined experimentally in transfected cell culture. In addition, splice sites of seven published polyomaviruses were analyzed. Based on these data, LTAg and STAg annotations were corrected for 10/86 and 74/86 published polyomaviruses, respectively. For 25 polyomaviruses, a spliced middle T CDS was observed or predicted. Splice sites that likely indicate expression of additional, alternative T antigens, were experimentally detected for six polyomaviruses. In contrast to all other mammalian polyomaviruses, three closely related cetartiodactyl polyomaviruses display two introns within their LTAg CDS. In addition, the VP2 of Glis glis (edible dormouse) polyomavirus 1 was observed to be encoded by a spliced transcript, a unique experimental finding within the Polyomaviridae family. Co-phylogenetic analyses based on LTAg CDS revealed a measurable signal of codivergence when considering all mammalian polyomaviruses, most likely driven by relatively recent codivergence events. Lineage duplication was the only other process whose influence on polyomavirus evolution was unambiguous. Finally, our analyses suggest that an update of the taxonomy of the family is required, including the creation of novel genera of mammalian and non-mammalian polyomaviruses.info:eu-repo/semantics/publishedVersio

Shoot & Copy: Using Mobile Phones for Accessing Information on Large Displays

Abstract. We present Shoot & Copy, an information transfer technique that does not require visual codes that interfere with shown content on large displays. By using Shoot & Copy users can capture an arbitrary sub-region of a large screen that contains pieces of data. The captured image is then analyzed and a reference to the corresponding data or the data itself is sent back to the mobile phone. When the user wants to view the captured information in more detail, the Shoot & Copy prototype allows retrieving the data on a standard personal computer or viewing data directly on the mobile phone. With this technique, displayed content in our everyday life is easily memorable by users. In addition, supplementary information can be transferred to the mobile phone.

Touch to Play – Exploring Touch-Based Mobile Interaction with Public Displays

Abstract—Mobile interactions with public displays are often indirect and not very convenient for multiple users at the same time. In this paper we use the physical, touch-based interaction with Near Field Communication (NFC) to investigate direct mobile interactions with public displays for multiple users. For that purpose, we adopt the Whack-a-Mole game for dynamic NFC-displays, which combine the physical interaction with NFCtagged objects and the visual output capabilities of public displays. We show that a grid of NFC-tags can be used to implement direct mobile interaction with public displays in general and a highly interactive multiplayer game in particular. Our study shows that users appreciate this physical, NFC-based mobile interaction, although technical advances are still necessary in order to improve its recognition rate of about 70%. The study also indicates that users are willing to interact with large displays in public, but prefer private or semi-public places, where their interactions attract less attention. we adopt the Whack-a-Mole game for dynamic NFC-displays. This technology uses a grid of NFC-tags as a physical user interface (UI) that users can touch with their mobile devices to manipulate an application UI that is projected onto the physical UI (Fig. 1). The physical interaction between individual mobile devices and NFC-tags enables a more direct interaction between mobile devices, large screens and their contents than public displays or touch screens. I