121 research outputs found
Experiences of Secondary School Teachers, Students, and Administrators in One Exemplary EcoSchool: A Description of Successful Practices and Challenges
This descriptive case study focused on the implementation strategies, experiences of teacher members, and involvement of students in an Ontario environmental education (EE) program called EcoSchools. Data were collected during the 2015–2016 academic year within a Gold certified secondary school in southern Ontario. Data sources included document analysis of 7 years of Eco certification portfolios, observations of EcoTeam activities, and semi-structured interviews with 15 teachers, administrators, and students. The study offered important insights into the successes and challenges of EcoSchools implementation. Successes include democratic EcoTeam dynamics, administrative support, student leadership opportunities, and rewarding greening projects in the school and local community. Data reveal major challenges with low student involvement in the EcoTeam and eco-projects, emphasizing participation over student learning, and a lack of engaging activities for secondary level students. Recommendations for schools include incorporating eco-goals into the school improvement plan, recruiting more student volunteers for school ground greening projects, planning environmental stewardship projects that engage the whole school community, and emphasizing the educational focus of eco-campaigns. Critical recommendations for Ontario EcoSchools program developers include revising activities to specially engage secondary school students, requiring an action plan so EcoSchools make data-driven decisions towards improvements, and promoting school-wide professional development to build capacity for all teachers. It is also recommended that student environmental literacy assessments be initiated across the province to build accountability for the Ontario EE policy framework and encourage EE to hold more of a priority position within secondary schools
Base-By-Base: Single nucleotide-level analysis of whole viral genome alignments
BACKGROUND: With ever increasing numbers of closely related virus genomes being sequenced, it has become desirable to be able to compare two genomes at a level more detailed than gene content because two strains of an organism may share the same set of predicted genes but still differ in their pathogenicity profiles. For example, detailed comparison of multiple isolates of the smallpox virus genome (each approximately 200 kb, with 200 genes) is not feasible without new bioinformatics tools. RESULTS: A software package, Base-By-Base, has been developed that provides visualization tools to enable researchers to 1) rapidly identify and correct alignment errors in large, multiple genome alignments; and 2) generate tabular and graphical output of differences between the genomes at the nucleotide level. Base-By-Base uses detailed annotation information about the aligned genomes and can list each predicted gene with nucleotide differences, display whether variations occur within promoter regions or coding regions and whether these changes result in amino acid substitutions. Base-By-Base can connect to our mySQL database (Virus Orthologous Clusters; VOCs) to retrieve detailed annotation information about the aligned genomes or use information from text files. CONCLUSION: Base-By-Base enables users to quickly and easily compare large viral genomes; it highlights small differences that may be responsible for important phenotypic differences such as virulence. It is available via the Internet using Java Web Start and runs on Macintosh, PC and Linux operating systems with the Java 1.4 virtual machine
Complementary and alternative medicine use in oncology: A questionnaire survey of patients and health care professionals
<p>Abstract</p> <p>Background</p> <p>We aimed to investigate the prevalence and predictors of Complementary and Alternative Medicine (CAM) use among cancer patients and non-cancer volunteers, and to assess the knowledge of and attitudes toward CAM use in oncology among health care professionals.</p> <p>Methods</p> <p>This is a cross-sectional questionnaire survey conducted in a single institution in Ireland. Survey was performed in outpatient and inpatient settings involving cancer patients and non-cancer volunteers. Clinicians and allied health care professionals were asked to complete a different questionnaire.</p> <p>Results</p> <p>In 676 participants including 219 cancer patients; 301 non-cancer volunteers and 156 health care professionals, the overall prevalence of CAM use was 32.5% (29.1%, 30.9% and 39.7% respectively in the three study cohorts). Female gender (p < 0.001), younger age (p = 0.004), higher educational background (p < 0.001), higher annual household income (p = 0.001), private health insurance (p = 0.001) and non-Christian (p < 0.001) were factors associated with more likely CAM use. Multivariate analysis identified female gender (p < 0.001), non-Christian (p = 0.001) and private health insurance (p = 0.015) as independent predictors of CAM use. Most health care professionals thought they did not have adequate knowledge (58.8%) nor were up to date with the best evidence (79.2%) on CAM use in oncology. Health care professionals who used CAM were more likely to recommend it to patients (p < 0.001).</p> <p>Conclusions</p> <p>This study demonstrates a similarly high prevalence of CAM use among oncology health care professionals, cancer and non cancer patients. Patients are more likely to disclose CAM usage if they are specifically asked. Health care professionals are interested to learn more about various CAM therapies and have poor evidence-based knowledge on specific oncology treatments. There is a need for further training to meet to the escalation of CAM use among patients and to raise awareness of potential benefits and risks associated with these therapies.</p
Structural colour in Chondrus crispus.
The marine world is incredibly rich in brilliant and intense colours. Photonic structures are found in many different species and provide extremely complex optical responses that cannot be achieved solely by pigments. In this study we examine the cuticular structure of the red alga Chondrus crispus (Irish Moss) using anatomical and optical approaches. We experimentally measure the optical response of the multilayer structure in the cuticle. Using finite-difference time-domain modelling, we demonstrate conclusively for the first time that the dimensions and organisation of lamellae are responsible for the blue structural colouration on the surface of the fronds. Comparison of material along the apical-basal axis of the frond demonstrates that structural colour is confined to the tips of the thalli and show definitively that a lack of structural colour elsewhere corresponds with a reduction in the number of lamellae and the regularity of their ordering. Moreover, by studying the optical response for different hydration conditions, we demonstrate that the cuticular structure is highly porous and that the presence of water plays a critical role in its ability to act as a structural light reflector.The research leading to these results has received funding from the BBSRC David Phillips fellowship (BBSRC David Phillips, BB/K014617/1). BJG thanks the Leverhulme Trust grant (F/09-741/G). RHW thanks the British Phycological Society for a Project Award (2012).This is the final version of the article. It first appeared from NPG via http://dx.doi.org/10.1038/srep1164
2017 Scientific Consensus Statement: land use impacts on the Great Barrier Reef water quality and ecosystem condition
This report provides the 2017 Scientific Consensus Statement for the Great Barrier Reef – a review of the significant advances in scientific knowledge of water quality issues in the Great Barrier Reef to arrive at a consensus on the current understanding of the system. The consensus statement was produced by a multidisciplinary group of scientists, with oversight from the Reef Independent Science Panel, and supports the development of the Reef 2050 Water Quality Improvement Plan 2017–2022
2017 Scientific Consensus Statement: land use impacts on the Great Barrier Reef water quality and ecosystem condition, Chapter 5: overview of key findings, management implications and knowledge gaps
To support the development of the Reef 2050 Water Quality Improvement Plan 2017-2022, a multidisciplinary group of scientists, with oversight from the Reef Independent Science Panel, was established to review and synthesise the significant advances in scientific knowledge of water quality issues in the Great Barrier Reef to arrive at a consensus on the current understanding of the system. For the 2017 Scientific Consensus Statement, the information and findings in these assessments and in other scientific publications were reviewed and synthesised in four supporting chapters. This fifth and final chapter provides a synthesis of the key findings of these four chapters and, based on this evidence, makes recommendations for future management of water quality in the Great Barrier Reef. The overarching consensus is that: Key Great Barrier Reef ecosystems continue to be in poor condition. This is largely due to the collective impact of land run-off associated with past and ongoing catchment development, coastal development activities, extreme weather events and climate change impacts such as the 2016 and 2017 coral bleaching events. Current initiatives will not meet the water quality targets. To accelerate the change in on-ground management, improvements to governance, program design, delivery and evaluation systems are urgently needed. This will require greater incorporation of social and economic factors, better targeting and prioritisation, exploration of alternative management options and increased support and resources
Heteronuclear bimetallic complexes with 3d and 4f elements
Three heteronuclear bimetallic complexes [Cu(MeOH)(L)Ln(NO3)3] ( 1-Ce ; Ln = Ce, 1-Pr ; Ln = Pr, and 1-Nd ; Ln = Nd) were prepared using H2L (1,3-bis[(3-methoxysalicylidene)amino]-2,2-dimethylpropane) in methanol, affording the complexes as green crystalline materials. These can be prepared in a one-pot synthesis from 2,2-dimethylpropan-1,3-diamine, o-vanillin, copper(II) nitrate, and Ln(III) nitrate (Ln = Ce, Pr, Nd). X-ray crystallography, high-resolution mass spectrometry, and UV-vis spectroscopy were used to characterize the bimetallic complexes. All three complexes showed the copper center adopting a five-coordinate square pyramidal geometry and the lanthanoid cation adopting a ten-coordinate geometry.Publisher PDFPeer reviewe
Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets
AbstractPorcine deltacoronavirus (PDCoV) was first identified in Hong Kong in 2009–2010 and reported in United States swine for the first time in February 2014. However, diagnostic tools other than polymerase chain reaction for PDCoV detection were lacking and Koch׳s postulates had not been fulfilled to confirm the pathogenic potential of PDCoV. In the present study, PDCoV peptide-specific rabbit antisera were developed and used in immunofluorescence and immunohistochemistry assays to assist PDCoV diagnostics. The pathogenicity and pathogenesis of PDCoV was investigated following orogastric inoculation of 5-day-old piglets with a plaque-purified PDCoV cell culture isolate (3×104 TCID50 per pig). The PDCoV-inoculated piglets developed mild to moderate diarrhea, shed increasing amount of virus in rectal swabs from 2 to 7 days post inoculation, and developed macroscopic and microscopic lesions in small intestines with viral antigen confirmed by immunohistochemistry staining. This study experimentally confirmed PDCoV pathogenicity and characterized PDCoV pathogenesis in neonatal piglets
2017 Scientific Consensus Statement: land use impacts on the Great Barrier Reef water quality and ecosystem condition, Chapter 3: the risk from anthropogenic pollutants to Great Barrier Reef coastal and marine ecosystems
In this chapter, we applied an ecological risk assessment approach to assess the likelihood of exposure and potential risks from land-based pollutants to Great Barrier Reef coastal (floodplain wetlands and floodplains) and marine (coral reefs and seagrass meadows) ecosystems. Ecological risk is defined as the product of the likelihood of an effect occurring and the consequences if that effect was to occur
- …