487 research outputs found
Extraordinarily high leaf selenium to sulfur ratios define āse-accumulatorā plants
Background and Aims: Selenium (Se) and sulfur (S) exhibit similar chemical properties. In flowering plants (angiosperms) selenate and sulfate are acquired and assimilated by common transport and metabolic pathways. It is hypothesized that most angiosperm species show little or no discrimination in the accumulation of Se and S in leaves when their roots are supplied a mixture of selenate and sulfate, but some, termed Se-accumulator plants, selectively accumulate Se in preference to S under these conditions.
Methods: This paper surveys Se and S accumulation in leaves of 39 angiosperm species, chosen to represent the range of plant Se accumulation phenotypes, grown hydroponically under identical conditions.
Results: The data show that, when supplied a mixture of selenate and sulfate: (1) plant species differ in both their leaf Se ([Se]leaf) and leaf S ([S]leaf) concentrations; (2) most angiosperms show little discrimination for the accumulation of Se and S in their leaves and, in non-accumulator plants, [Se]leaf and [S]leaf are highly correlated; (3) [Se]leaf in Se-accumulator plants is significantly greater than in other angiosperms, but [S]leaf, although high, is within the range expected for angiosperms in general; and (4) the Se/S quotient in leaves of Se-accumulator plants is significantly higher than in leaves of other angiosperms.
Conclusion: The traits of extraordinarily high [Se]leaf and leaf Se/S quotients define the distinct elemental composition of Se-accumulator plants
Soil and foliar zinc biofortification of broccolini: effects on plant growth and mineral accumulation
Ā© 2020 CSIRO. Millions of people have Zn-deficient diets, so Zn-biofortified crops could prevent such deficiency. The aim of this study was to evaluate the use of agronomic Zn biofortification of broccolini-a new hybrid crop variety derived from a cross between kalian cabbage and broccoli. Plants were grown in pots using a Zn deficient soil. Four fertiliser treatments were tested: (1) control (2) soil application of 5 mg ZnSO4ā¢7H2O kg-1 soil (3) foliar application at the early flowering stage of 0.5% (w/v) ZnSO4ā¢7H2O (4) combined soil and foliar treatments. Florets were harvested in four sequential harvests. There was a decrease in both growth and leaf composition of Zn, Ca, Fe and Mg. Soil Zn application increased floret production. There were increases in the Zn concentration stem+leaves and florets of 12- and 2.5-fold in foliar and soil+foliar treatments respectively. PA:Zn molar ratios decreased under both foliar and soil+foliar treatments. Boiling reduced Zn concentration by 40%, along with a decrease of other mineral nutrients. A soil+foliar treatment can increase both plant growth and Zn concentration in broccolini, and boiled 100 g portion of biofortified florets fertilised at rates in this study would deliver ā¼49 mg Zn, a 46% increase than in the non-biofortified broccolini
Soil and foliar zinc application to biofortify broccoli (Brassica oleracea var. Italica L.): Effects on the zinc concentration and bioavailability
Ā© 2020, Czech Academy of Agricultural Sciences. All rights reserved. Agronomic zinc (Zn) biofortification of crops could help to alleviate dietary Zn deficiency, which is likely to affect more than one billion people worldwide. To evaluate the efficiency of agronomic Zn biofortification of broc-coli, four application treatments were tested: no Zn application (control); soil application of 5 mg/kg ZnSO4Ā·7 H2O (soil); two sprays (15 mL/pot each) of 0.25% (w/v) ZnSO4Ā·7 H2O (foliar); and soil + foliar combination. Soil Zn application increased Zn-DTPA (diethylenetriamine pentaacetic acid) concentration by 3.7-times but did not affect plant growth or plant Zn concentration. Foliar Zn application increased stem + leaves and floret Zn concentration by 78 and 23 mg Zn/kg, respectively, with good bioavailability based on phytic acid concentration. Boiling decreased mineral concentration by 19%, but increased bioavailability by decreasing the phytic acid concentration. The entire broccoli could constitute a good nutritional source for animals and humans. An intake of 100 g boiled florets treated with the foliar treatment will cover about 36% of recommended dietary intake (RDI) of Zn, together with 30% of Ca, 94% of K, 32% of Mg, 6% of Na, 55% of P, 60% of S, 10% of Cu, 22% of Fe, 43% of Mn, and 35% of Se RDIs
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Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection
Background: Affymetrix GeneChip arrays are widely used for transcriptomic studies in a diverse range of species. Each gene is represented on a GeneChip array by a probe-set, consisting of up to 16 probe-pairs. Signal intensities across probe-pairs within a probe-set vary in part due to different physical hybridisation characteristics of individual probes with their target labelled transcripts. We
have previously developed a technique to study the transcriptomes of heterologous species based
on hybridising genomic DNA (gDNA) to a GeneChip array designed for a different species, and subsequently using only those probes with good homology.
Results: Here we have investigated the effects of hybridising homologous species gDNA to study the transcriptomes of species for which the arrays have been designed. Genomic DNA from Arabidopsis thaliana and rice (Oryza sativa) were hybridised to the Affymetrix Arabidopsis ATH1 and Rice Genome GeneChip arrays respectively. Probe selection based on gDNA hybridisation
intensity increased the number of genes identified as significantly differentially expressed in two
published studies of Arabidopsis development, and optimised the analysis of technical replicates obtained from pooled samples of RNA from rice.
Conclusion: This mixed physical and bioinformatics approach can be used to optimise estimates of gene expression when using GeneChip arrays
The influence of style and origin on mineral composition of beers retailing in the UK
Beer has high nutritional values in terms of energy and is also a dietary source of antioxidants, carbohydrates and minerals among others. In Europe, 53 Mt of beer are produced annually, and with an average supply of 68.2 kg capitaā¾Ā¹ yearā¾Ā¹ among adults. In this study, the mineral composition of 125 commercial beer samples retailing in the UK, but originating from ten countries, was determined; such detailed information is lacking in UK food composition tables. Beer composition data are reported for Al, As, Ba, Ca, Cd, Co, Cr, Cs, Cu, Fe, K, Mg, Mn, Mo, Na, Ni, Pb, Se, Sr, U, V and Zn, following analysis by inductively coupled plasma mass spectrometry. ANOVA results showed higher concentrations of Mo, Pb and Sr (0.160; 491.70 Ć 10ā¾āµ; 0.38 mg Lā¾Ā¹, respectively) for stout/porter style and a significant higher amount of minerals such as Al (3.835 mg Lā1), Cd (8.64 Ć 10ā5 mg Lā¾Ā¹), Mn (1.02 mg Lā¾Ā¹) or Ni (0.312 mg Lā¾Ā¹) among others for lambic beer. Regarding the country of origin, higher Se concentrations were reported from beer brewed in the USA (0.110 mg Lā¾Ā¹). It is concluded that beer style was determined to have a greater effect on beer mineral composition than origin or container type
Transforming higher education and student engagement through collaborative review to inform educational design
This paper reports on staff perceptions arising from a review process designed to assist staff in making informed decisions regarding educational design, approaches to engage students in learning, and the technology to support engagement in the classroom and across multiple locations and delivery modes. The aim of the review process was to transform the level of student engagement in the business faculty of an Australian university. The process took a collaborative approach through consultation with academic staff involved in the design and delivery of the units under review, and included targeted professional development as necessary. An institutional framework that characterises engagement indicator contexts and their attributes facilitated dialog during the review process. This paper reports on a mixed method study that included a survey of participants, and purposeful interviews to evaluate the effectiveness of the process. Although the study identified factors that hindered implementation and operationalization of review recommendations in some instances, study participants were generally of the view that recommendations would enhance student engagement. It is demonstrated that the bottom-up approach described in this paper is consistent with theoretical frameworks for transformational change in teaching and learning and the adoption of innovations
Heat and salinity stress on the African eggplant F1 Djamba, a Kumba cultivar
Climate change is expected to increase soil salinity and heat-wave intensity, duration, and frequency. These stresses, often present in combination, threaten food security as most common crops do not tolerate them. The African eggplant (Solanum aethiopicum L.) is a nutritious traditional crop found in sub-Saharan Africa and adapted to local environments. Its wider use is, however, hindered by the lack of research on its tolerance. This project aimed to describe the effects of salinity (100 mM NaCl solution) combined with elevated temperatures (27/21Ā°C, 37/31Ā°C, and 42/36Ā°C). High temperatures reduced leaf biomass while cell membrane stability was reduced by salinity. Chlorophyll levels were boosted by salinity only at the start of the stress with only the different temperatures significantly impacted the levels at the end of the experiment. Other fluorescence parameters such as maximum quantum yield and non-photochemical quenching were only affected by the temperature change. Total antioxidants were unchanged by either stress despite a decrease of phenols at the highest temperature. Leaf sodium concentration was highly increased by salinity but phosphorus and calcium were unchanged by this stress. These findings shed new light on the tolerance mechanisms of the African eggplant under salinity and heat. Further research on later developmental stages is needed to understand its potential in the field in areas affected by these abiotic stresses
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Using genomic DNA-based probe-selection to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species
High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChipĀ® technology are amongst the most widely used, although GeneChipĀ® arrays are currently available for only a small number of plant and animal species. Thus, we have developed a method to improve the sensitivity of high-density oligonucleotide arrays when applied to heterologous species and tested the method by analysing the transcriptome of Brassica oleracea L., a species for which no GeneChipĀ® array is available, using a GeneChipĀ® array designed for Arabidopsis thaliana (L.) Heynh. Genomic DNA from B. oleracea was labelled and hybridised to the ATH1-121501 GeneChipĀ® array. Arabidopsis thaliana probe-pairs that hybridised to the B. oleracea genomic DNA on the basis of the perfect-match (PM) probe signal were then selected for subsequent B. oleracea transcriptome analysis using a .cel file parser script to generate probe mask files. The transcriptional response of B. oleracea to a mineral nutrient (phosphorus; P) stress was quantified using probe mask files generated for a wide range of gDNA hybridisation intensity thresholds. An example probe mask file generated with a gDNA hybridisation intensity threshold of 400 removed > 68 % of the available PM probes from the analysis but retained >96 % of available A. thaliana probe-sets. Ninety-nine of these genes were then identified as significantly regulated under P stress in B. oleracea, including the homologues of P stress responsive genes in A. thaliana. Increasing the gDNA hybridisation intensity thresholds up to 500 for probe-selection increased the sensitivity of the GeneChipĀ® array to detect regulation of gene expression in B. oleracea under P stress by up to 13-fold. Our open-source software to create probe mask files is freely available http://affymetrix.arabidopsis.info/xspecies/ webcite and may be used to facilitate transcriptomic analyses of a wide range of plant and animal species in the absence of custom arrays
Zinc fertilization increases productivity and grain nutritional quality of cowpea (Vigna unguiculata [L.] Walp.) under integrated soil fertility management
Cowpea (Vigna unguiculata [L.] Walp.) is an important but under-studied grain legume which can potentially contribute to improved dietary zinc (Zn) intake in sub-Saharan Africa. In this study, surveys were conducted on smallholder farms in Zimbabwe during 2014/15 to determine the influence of diverse soil fertility management options on cowpea grain productivity and nutrition quality. Guided by the surveys, field experiments were conducted to investigate the influence of Zn fertilizer on the productivity and quality of cowpea under integrated soil fertility management (ISFM). Experiments were conducted on two soil-types, namely, sandy (6% clay) and red clay (57% clay) in 2014/15 and 2015/16 where cowpea was grown in rotation with staple maize (Zea mays L.) and fertilized with combinations of Zn, nitrogen (N), phosphorus (P) and two organic nutrient resources, cattle manure and woodland leaf litter. Cowpea grain yields on surveyed farms ranged from 0.3 to 0.9 t haā1, with grain Zn concentration ranging from 23.9 to 30.1 mg kgā1. The highest grain Zn concentration was on fields where organic nutrient resources were applied in combination with mineral N and P fertilizers. Within the field experiments, mean grain yields of cowpea increased by between 12 and 18% on both soil types when Zn fertilizer was applied, from a baseline of 1.6 and 1.1 t haā1 on red clay and sandy soils, respectively. When Zn fertilizer was co-applied with organic nutrient resources, grain Zn concentrations of cowpea reached 42.1 mg kgā1 (red clay) and 44.7 mg kgā1 (sandy) against grain Zn concentrations of 35.9 mg kgā1 and 31.1 mg kgā1 measured in cowpea grown with no Zn fertilizer on red clay and sandy soils, respectively. Agronomic biofortification of legumes is feasible and has the potential to contribute significantly towards increasing dietary Zn intake by humans. A greater increase in grain Zn of cowpea grown on sandy than red clay soils under Zn fertilization illustrates the influence of soil type on Zn uptake, which should be explored further in agronomic biofortification programs
Drought and heatwave affected the African eggplant differently when present in combination than individually
Heat waves and drought periods are set to happen more often due to a changing climate, especially in Africa. The African eggplant, Solanum aethiopicum L., is a highly nutritious vegetable crop indigenous to Africa commonly grown by small-holder farmers. It is adapted to harsh environments but empirical data on its tolerance to high temperatures and drought individually or in combination to help farmers is lacking. This project aimed to describe the effects of drought in combination with heat waves on the leafy African eggplant Shum E11 genotype over 26 days of stress. Four-week-old plants were subjected to repetitive heatwave-type stress consisting of eight days of day/night temperatures reaching 32/26Ā°C or 37/31Ā°C compared to 27/21Ā°C, followed by four days of recovery, repeated twice. A drought treatment was applied during the experiment by maintaining the soil water potential at -100 kPa compared with -10 kPa for non-stressed plants. Drought stress had a predominant effect over heat by reducing leaf production and leaf membrane stability and increasing chlorophyll content. Antioxidants and phenols were unaffected by drought or heat stress while mineral elements were impacted differently by each stress and their combination. These results indicate a good tolerance of the E11 genotype to heat stress during the vegetative stage while drought negatively affected plant growth and leaf biochemical compounds. Further research on later developmental stages will complete our understanding of this variety's tolerance to heat and drought and its potential role in fields affected by environmental stresses
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