109 research outputs found
Driven Assembly of Lignin into Microcapsules for Storage and Delivery of Hydrophobic Molecules
Oil-filled microcapsules of kraft lignin were synthe-
sized by first creating an oil in water emulsion followed by a high-
intensity, ultrasound-assisted cross-linking of lignin at the water/oil
interface. The rationale behind our approach is based on promoting
documented lignin hydrophobic interactions within the oil phase,
followed by locking the resulting spherical microsystems by covalent
cross-linking using a high intensity ultrasound treatment. As further
evidence in support of our rationale, confocal and optical
microscopies demonstrated the uniformly spherical morphology of
the created lignin microparticles. The detailed elucidation of the
cross-linking processes was carried out using gel permeation
chromatography (GPC) and quantitative 31P NMR analyses. The
ability of lignin microcapsules to incorporate and release Coumarin-6
was evaluated in detail. In vitro studies and confocal laser scanning microscopy analysis were carried out to assess the internalization of capsules into Chinese hamster ovary (CHO) cells. This part of our work demonstrated that the lignin microcapsules are not cytotoxic and readily incorporated in the CHO cells
Synthetic Nanoparticles for Vaccines and Immunotherapy
The immune system plays a critical role in our health. No other component of human physiology plays a decisive role in as diverse an array of maladies, from deadly diseases with which we are all familiar to equally terrible esoteric conditions: HIV, malaria, pneumococcal and influenza infections; cancer; atherosclerosis; autoimmune diseases such
as lupus, diabetes, and multiple sclerosis. The importance of understanding the function of the immune system and learning how to modulate immunity to protect against or treat disease thus cannot be overstated. Fortunately, we are entering an exciting era where the
science of immunology is defining pathways for the rational manipulation of the immune system at the cellular and molecular level, and this understanding is leading to dramatic advances in the clinic that are transforming the future of medicine.1,2 These initial advances are being made primarily through biologic drugs– recombinant proteins (especially antibodies) or patient-derived cell therapies– but exciting data from preclinical studies suggest that a marriage of approaches based in biotechnology with the materials science and chemistry of nanomaterials, especially nanoparticles, could enable more effective and safer immune engineering strategies. This review will examine these nanoparticle-based strategies to immune modulation in detail, and discuss the promise and outstanding challenges facing the field of immune engineering from a chemical biology/materials engineering perspectiveNational Institutes of Health (U.S.) (Grants AI111860, CA174795, CA172164, AI091693, and AI095109)United States. Department of Defense (W911NF-13-D-0001 and Awards W911NF-07-D-0004
An Evolutionary Upgrade of Cognitive Load Theory: Using the Human Motor System and Collaboration to Support the Learning of Complex Cognitive Tasks
Cognitive load theory is intended to provide instructional strategies derived from experimental, cognitive load effects. Each effect is based on our knowledge of human cognitive architecture, primarily the limited capacity and duration of a human working memory. These limitations are ameliorated by changes in long-term memory associated with learning. Initially, cognitive load theory's view of human cognitive architecture was assumed to apply to all categories of information. Based on Geary's (Educational Psychologist 43, 179-195 2008; 2011) evolutionary account of educational psychology, this interpretation of human cognitive architecture requires amendment. Working memory limitations may be critical only when acquiring novel information based on culturally important knowledge that we have not specifically evolved to acquire. Cultural knowledge is known as biologically secondary information. Working memory limitations may have reduced significance when acquiring novel
The gestural misinformation effect : skewing eyewitness testimony through gesture
The susceptibility of eyewitnesses to verbal suggestion has been well documented, although little attention has been paid to the role of nonverbal communication in misinformation. Three experiments are reported; in each, participants watched footage of a crime scene before being questioned about what they had observed. In Experiments 1 and 2, an on-screen interviewer accompanied identically worded questions with gestures that either conveyed accurate information about the scene or conveyed false, misleading information. The misleading gestures significantly influenced recall, and participants' responses were consistent with the gestured information. In Experiment 3, a live interview was conducted, and the gestural misinformation effect was found to be robust; participants were influenced by misleading gestures performed by the interviewer during questioning. These findings provide compelling evidence for the gestural misinformation effect, whereby subtle hand gestures can implant information and distort the testimony of eyewitnesses. The practical and legal implications of these findings are discussedPeer reviewe
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