Transcriptome Analysis of \u3ci\u3eBifidobacterium Longum\u3c/i\u3e Strains that Show a Differential Response to Hydrogen Peroxide Stress

Consumer and commercial interest in foods containing probiotic bifidobacteria is increasing. However, because bifidobacteria are anaerobic, oxidative stress can diminish cell viability during production and storage of bioactive foods. We previously found Bifidobacterium longum strain NCC2705 had signifi- cantly greater intrinsic and inducible resistance to hydrogen peroxide (H2O2) than strain D2957. Here, we explored the basis for these differences by examining the transcriptional responses of both strains to sub-lethal H2O2 exposure for 5- or 60-min. Strain NCC2705 had 288 genes that were differentially expressed after the 5-min treatment and 114 differentially expressed genes after the 60-min treatment. In contrast, strain D2957 had only 21 and 90 differentially expressed genes after the 5- and 60-min treatments, respectively. Both strains showed up-regulation of genes coding enzymes implicated in oxidative stress resistance, such as thioredoxin, thioredoxin reductase, peroxiredoxin, ferredoxin, glutaredoxin, and anaerobic ribonucleotide reductase, but induction levels were typically highest in NCC2705. Compared to D2957, NCC2705 also had more up-regulated genes involved in transcriptional regulation and more down-regulated genes involved in sugar transport and metabolism. These results provide a greater understanding of the molecular basis for oxidative stress resistance in B. longum and the factors that contribute to strain-to-strain variability in survival in bioactive food products

Genetic and Physiological Responses of \u3ci\u3eBifidobacterium animalis\u3c/i\u3e subsp. \u3ci\u3elactis\u3c/i\u3e to Hydrogen Peroxide Stress

Consumer interest in probiotic bifidobacteria is increasing, but industry efforts to secure high cell viability in foods is determined by these anaerobes’ sensitivity to oxidative stress. To address this limitation, we investigated genetic and physiological responses of two fully sequenced Bifidobacterium animalis subsp. lactis strains, BL-04 and DSM 10140, to hydrogen peroxide (H2O2) stress. Although the genome sequences for these strains are highly clonal, prior work showed they differ in both intrinsic and inducible H2O2 resistance. Transcriptome analysis of early stationary phase cells exposed to a sub-lethal H2O2 concentration detected significant (P2O2 stress resistance might be due to a mutation in a BL-04 gene encoding long chain fatty acid-coA ligase. To explore this possibility, membrane fatty acids were isolated and analyzed by GC-MS. Results confirmed the strains had significantly different lipid profiles; the BL-04 membrane contained higher percentages of C14:0 and C16:0, and lower percentages of C16:1n7 and C18:1n9. Alteration of the DSM 10140 membrane lipid composition using modified growth medium to more closely mimic that of BL-04 yielded cells that showed increased intrinsic resistance to lethal H2O2 challenge, but did not display an inducible H2O2 stress response. Results show deliberate stress induction or membrane lipid modification can be employed to significantly improve H2O2 resistance in B. animalis subsp. lactis strains

Phase variation controls expression of Salmonella lipopolysaccharide modification genes by a DNA methylation-dependent mechanism

The O-antigen of Salmonella lipopolysaccharide is a major antigenic determinant and its chemical composition forms the basis for Salmonella serotyping. Modifications of the O-antigen that can affect the serotype include those carried out by the products of glycosyltransferase operons (gtr), which are present on specific Salmonella and phage genomes. Here we show that expression of the gtr genes encoded by phage P22 that confers the O1 serotype is under the control of phase variation. This phase variation occurs by a novel epigenetic mechanism requiring OxyR in conjunction with the DNA methyltransferase Dam. OxyR is an activator or a repressor of the system depending on which of its two binding sites in the gtr regulatory region is occupied. Binding is decreased by methylation at Dam target sequences in either site, and this confers heritability of the expression state to the system. Most Salmonella gtr operons share the key regulatory elements that are identified here as essential for this epigenetic phase variation

Psychosocial predictors of exclusive breastfeeding duration to six moths postpartum

The World Health Organization recommend that for optimal growth and development all infants worldwide should be exclusively breastfed for the first six months of life. Previous studies have identified that psychosocial factors are important for the maintenance of exclusive breastfeeding. This study aimed to examine the psychosocial factors associated with exclusive breastfeeding duration to six months postpartum. Method: 125 women completed questionnaires at three time points; 32 weeks gestation, two and six months postpartum. Self-efficacy, body attitude, psychological adjustment, attitude, intention, confidence, motivation and importance of exclusive breastfeeding and breastfeeding status were measured. Findings: At 32 weeks gestation a woman’s confidence to achieve exclusive breastfeeding was a predictor of exclusive breastfeeding to six months postpartum. At two months postpartum, psychological adjustment and self-efficacy were predictive of exclusive breastfeeding duration. At six months postpartum, psychological adjustment, self-efficacy, confidence to maintain and feeling fat were predictive of exclusive breastfeeding duration. Conclusion: Self-efficacy, psychological adjustment, body image, motivation and confidence are important for the maintenance of exclusive breastfeeding to six months. This has clinical application for health psychology as antenatal breastfeeding education and postpartum support may be strengthened by strategies that build a woman’s confidence to exclusively breastfeed

Optimal growth ofLactobacillus casei in a Cheddar cheese ripening model system requires exogenous fatty acids

Flavor development in ripening Cheddar cheese depends on complex microbial and biochemical processes that are difficult to study in natural cheese. Thus, our group has developed Cheddar cheese extract (CCE) as a model system to study these processes. In previous work, we found that CCE supported growth of Lactobacillus casei, one of the most prominent nonstarter lactic acid bacteria (NSLAB) species found in ripening Cheddar cheese, to a final cell density of 108 cfu/mL at 37°C. However, when similar growth experiments were performed at 8°C in CCE derived from 4-mo-old cheese (4mCCE), the final cell densities obtained were only about 106 cfu/mL, which is at the lower end of the range of the NSLAB population expected in ripening Cheddar cheese. Here, we report that addition of Tween 80 to CCE resulted in a significant increase in the final cell density of L. casei during growth at 8°C and produced concomitant changes in cytoplasmic membrane fatty acid (CMFA) composition. Although the effect was not as dramatic, addition of milk fat or a monoacylglycerol (MAG) mixture based on the MAG profile of milk fat to 4mCCE also led to an increased final cell density of L. casei in CCE at 8°C and changes in CMFA composition. These observations suggest that optimal growth of L. casei in CCE at low temperature requires supplementation with a source of fatty acids (FA). We hypothesize that L. casei incorporates environmental FA into its CMFA, thereby reducing its energy requirement for growth. The exogenous FA may then be modified or supplemented with FA from de novo synthesis to arrive at a CMFA composition that yields the functionality (i.e., viscosity) required for growth in specific conditions. Additional studies utilizing the CCE model to investigate microbial contributions to cheese ripening should be conducted in CCE supplemented with 1% milk fat

Psychosocial predictors of exclusive breastfeeding duration to six moths postpartum

The World Health Organization recommend that for optimal growth and development all infants worldwide should be exclusively breastfed for the first six months of life. Previous studies have identified that psychosocial factors are important for the maintenance of exclusive breastfeeding. This study aimed to examine the psychosocial factors associated with exclusive breastfeeding duration to six months postpartum. Method: 125 women completed questionnaires at three time points; 32 weeks gestation, two and six months postpartum. Self-efficacy, body attitude, psychological adjustment, attitude, intention, confidence, motivation and importance of exclusive breastfeeding and breastfeeding status were measured. Findings: At 32 weeks gestation a woman’s confidence to achieve exclusive breastfeeding was a predictor of exclusive breastfeeding to six months postpartum. At two months postpartum, psychological adjustment and self-efficacy were predictive of exclusive breastfeeding duration. At six months postpartum, psychological adjustment, self-efficacy, confidence to maintain and feeling fat were predictive of exclusive breastfeeding duration. Conclusion: Self-efficacy, psychological adjustment, body image, motivation and confidence are important for the maintenance of exclusive breastfeeding to six months. This has clinical application for health psychology as antenatal breastfeeding education and postpartum support may be strengthened by strategies that build a woman’s confidence to exclusively breastfeed

Equivalence of operator-splitting schemes for the integration of the Langevin equation

We investigate the equivalence of different operator-splitting schemes for the integration of the Langevin equation. We consider a specific problem, so called the directed percolation process, which can be extended to a wider class of problems. We first give a compact mathematical description of the operator-splitting method and introduce two typical splitting schemes that will be useful in numerical studies. We show that the two schemes are essentially equivalent through the map that turns out to be an automorphism. An associated equivalent class of operator-splitting integrations is also defined by generalizing the specified equivalence.Comment: 4 page

Trajectories of dementia-related cognitive decline in a large mental health records derived patient cohort

BACKGROUND: Modeling trajectories of decline can help describe the variability in progression of cognitive impairment in dementia. Better characterisation of these trajectories has significant implications for understanding disease progression, trial design and care planning. METHODS: Patients with at least three Mini-mental State Examination (MMSE) scores recorded in the South London and Maudsley NHS Foundation Trust Electronic Health Records, UK were selected (N = 3441) to form a retrospective cohort. Trajectories of cognitive decline were identified through latent class growth analysis of longitudinal MMSE scores. Demographics, Health of Nation Outcome Scales and medications were compared across trajectories identified. RESULTS: Four of the six trajectories showed increased rate of decline with lower baseline MMSE. Two trajectories had similar initial MMSE scores but different rates of decline. In the faster declining trajectory of the two, a higher incidence of both behavioral problems and sertraline prescription were present. CONCLUSIONS: We find suggestive evidence for association of behavioral problems and sertraline prescription with rate of decline. Further work is needed to determine whether trajectories replicate in other datasets

Generalized Flow and Determinism in Measurement-based Quantum Computation

We extend the notion of quantum information flow defined by Danos and Kashefi for the one-way model and present a necessary and sufficient condition for the deterministic computation in this model. The generalized flow also applied in the extended model with measurements in the X-Y, X-Z and Y-Z planes. We apply both measurement calculus and the stabiliser formalism to derive our main theorem which for the first time gives a full characterization of the deterministic computation in the one-way model. We present several examples to show how our result improves over the traditional notion of flow, such as geometries (entanglement graph with input and output) with no flow but having generalized flow and we discuss how they lead to an optimal implementation of the unitaries. More importantly one can also obtain a better quantum computation depth with the generalized flow rather than with flow. We believe our characterization result is particularly essential for the study of the algorithms and complexity in the one-way model.Comment: 16 pages, 10 figure