Obtención de suero anti-Rice stripe necrosis virus por expresión heteróloga de la capside proteica viral

PosterEl entorchamiento del arroz”, causado por el Rice stripe necrosis virus ( es una gran amenaza para el cultivo del arroz en Argentina Hasta la fecha, se desconoce su distribución, cultivares promisoriamente resistentes y no se cuenta con un suero comercial para su detección El objetivo de este trabajo fue la obtención de suero anti RSNV para la detección del virus en un gran número de muestras en simultaneo y de bajo costo.Instituto de Patología VegetalFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bangratz, M. INRAE. CIRAD. Institut de Recherche pour le Développement (IRD); FranciaFil: Pinel Galzi, A. INRAE. CIRAD. Institut de Recherche pour le Développement (IRD); FranciaFil: Brizard, J.P. INRAE. CIRAD. Institut de Recherche pour le Développement (IRD); FranciaFil: Hébrard, E. INRAE. CIRAD. Institut de Recherche pour le Développement (IRD); FranciaFil: Brugidou, C. INRAE. CIRAD. Institut de Recherche pour le Développement (IRD); Franci

Deux stratégies d'échantillonnage pour une vue d'ensemble de la contamination des eaux douces par les pesticides au sein d'un bassin versant agricole

International audienceTwo headwaters located in southwest France were monitored for 3 and 2 years (Auvézère and Aixette watershed, respectively) with two sampling strategies: grab and passive sampling with polar organic chemical integrative sampler (POCIS). These watersheds are rural and characterized by agricultural areas with similar breeding practices, except that the Auvézère watershed contains apple production for agricultural diversification and the downstream portion of the Aixette watershed is in a peri-urban area. The agricultural activities of both are extensive, i.e., with limited supply of fertilizer and pesticides. The sampling strategies used here give specific information: grab samples for higher pesticide content and POCIS for contamination background noise and number of compounds found. Agricultural catchments in small headwater streams are characterized by a background noise of pesticide contamination in the range of 20-70 ng/L, but there may also be transient and high-peak pesticide contamination (2000-3000 ng/L) caused by rain events, poor use of pesticides, and/or the small size of the water body. This study demonstrates that between two specific runoff events, contamination was low; hence the importance of passive sampler use. While the peak pesticide concentrations seen here are a toxicity risk for aquatic life, the pesticide background noise of single compounds do not pose obvious acute nor chronic risks; however, this study did not consider the risk from synergistic "cocktail" effects. Proper tools and sampling strategies may link watershed activities (agricultural, non-agricultural) to pesticides detected in the water, and data from both grab and passive samples can contribute to discussions on environmental effects in headwaters, an area of great importance for biodiversity

Secretome of animal trypanosomes from a standard method toward new diagnostic and therapeutic targets

International audienceAnimal trypanosomosis is one of the most severe constraints to agricultural development in sub-Saharan Africa and is also an important disease of livestock in Latin America and Asia. The causative agents are various species of protozoan parasites belonging to the genus Trypanosoma, among which T. congolense and T. evansi are the major pathogenic species. The extracellular position of trypanosomes obliges us to consider both the parasite and its excreted/secreted factors in the course of the physiopathologic process. The advent of proteomics led us to propose a comparative approach of the proteome (i.e., the whole parasite content) and the secretome (i.e., naturally excreted/secreted molecules) of T. congolense and T. evansi with particular attention to common and specific molecules between strains of differing virulence and pathogenicity. The molecular identification of differentially expressed trypanosome molecules correlated with either the virulence process or the pathogenicity will provide new potential molecular targets for improved field diagnosis and chemotherapy of animal trypanosomosi