Additional file 3: Figure S2. of Spatial pattern of genetic diversity and selection in the MHC class II DRB of three Neotropical bat species

Positions of PCR primers used to amplify the indicated fragments of the MHC class II loci in C. perspicillata, D. rotundus and M. molossus, based on cDNA. The structure of the cDNA of the MHC class II DRB gene is based on [50]. According to each species, boxes indicate the amplified region using each couple of primers. The shaded region represents the region of interest, namely exon 2. Sequences and references of all primers used are given in Table 1. (PDF 26 kb

Additional file 1: Table S1. of Spatial pattern of genetic diversity and selection in the MHC class II DRB of three Neotropical bat species

Supplementary methods, including the characteristics of all capture sites (Table S1)ďťż, the list of samples successfully amplified for the MHC class II DRB loci (Table S2), the distribution of DRB alleles per species (Table S3-5), the pairwise differentiation computations per species (Table S6-8), as well as the correlations of genetics and geographic distance (Table S9). (XLS 179 kb

Quemere_gh_sequence_data

This file contains all unique sequences produced by G/H primers and occurring more than once. The sequences have been produced by the Illumina technology (GA IIx platform)

Faeces and plant sampling schemes.

<p>Faeces and plant sampling schemes.</p

Plant richness rarefaction curve.

<p>The solid line represents the cumulated number of MOTUs (a) and food plant genera (b) as a function of the number of sampled individuals. The dashed lines are the 95% confidence intervals.</p

Taxonomic resolution of the golden-crowned sifaka diet.

<p>Panel (a) presents the results base on the EMBL reference database only. Panel (b) presents the results using both EMBL and customized local reference database. Panel (c) uses the final results integrating the final validation by taxonomic experts. The proportions correspond to the number of sequences assigned to each taxonomic rank compared to the total number of identified sequences from DNA Barcoding (N = 130).</p

Environmental determinants of diet composition.

<p>Biplots of the co-inertia analysis between the PCA of diet composition (presence/absence of the 130 taxa) and the PCA of landscape structure (% area of the six habitats within the circular buffers).</p

Relative proportion of the most important habitat types within <i>P. tattersalli</i> home ranges.

<p>Proportions were calculated within circular buffers of 500m of radius centered on the group sampling location. N_maj indicate the number of groups for which this habitat is the most represented.</p

Quemere_gh_final

This file contains the filtered sequences produced by G/H primers. The sequences have been produced by the Illumina technology (GA IIx platform)

Quemere_gh_final_data

This file contains the filtered sequences produced by G/H primers. The sequences have been produced by the Illumina technology (GA IIx platform)