The Revolution Will Be Televised But Not Supported: Student Protest at Marquette University

This project examines the factors that affect the presence of student activism and student protest at Marquette University. The current social and political environment of the United States has created an exigence to discuss how students at Marquette critique their surroundings. Data is collected from a variety of sources including the author’s auto-ethnography, a review of historical and scholarly data, institutional data, and student and faculty interviews. Overall, the data shows that student protest and student activism at Marquette University exists amid a series of conflicting influences. The prioritization of donor-based funding and positive publicity, the ambiguity in the meaning of Marquette values, and the prevalence of repressive tolerance create an environment in which meaningful student activism is stifled.https://epublications.marquette.edu/english_3210ur/1036/thumbnail.jp

The effect of the annealing temperature on the local distortion of La0.67_{0.67}Ca0.33_{0.33}MnO3_3 thin films

Mn $K$-edge fluorescence data are presented for thin film samples (3000~\AA) of Colossal Magnetoresistive (CMR) La$_{0.67}$Ca$_{0.33}$MnO$_3$: as-deposited, and post-annealed at 1000 K and 1200 K. The local distortion is analyzed in terms of three contributions: static, phonon, and an extra, temperature-dependent, polaron term. The polaron distortion is very small for the as-deposited sample and increases with the annealing temperature. In contrast, the static distortion in the samples decreases with the annealing temperature. Although the local structure of the as-deposited sample shows very little temperature dependence, the change in resistivity with temperature is the largest of these three thin film samples. The as-deposited sample also has the highest magnetoresistance (MR), which indicates some other mechanism may also contribute to the transport properties of CMR samples. We also discuss the relationship between local distortion and the magnetization of the sample.Comment: 11 pages of Preprint format, 8 figures in one tar fil

Should I Stay or Should I Go? Firm Heterogeneity in the Post-crisis Period

Existing microeconomic research on exporting firms is dominated by empirical findings across time and countries based on two theories of why firms choose to export. One requires firms to be better performers before entry, the other requires there to be improvements in performance as a result of entry. In this paper, we disentangle entry to, and exit from, the overseas market for UK manufacturing firms to better understand the motivations and characteristics underlying both decisions. We explore the extent to which changes in the macroeconomic environment may influence behaviour, following a time of global financial turbulence

Methods for high-dimensonal analysis of cells dissociated from cyropreserved synovial tissue

Background: Detailed molecular analyses of cells from rheumatoid arthritis (RA) synovium hold promise in identifying cellular phenotypes that drive tissue pathology and joint damage. The Accelerating Medicines Partnership RA/SLE Network aims to deconstruct autoimmune pathology by examining cells within target tissues through multiple high-dimensional assays. Robust standardized protocols need to be developed before cellular phenotypes at a single cell level can be effectively compared across patient samples. Methods: Multiple clinical sites collected cryopreserved synovial tissue fragments from arthroplasty and synovial biopsy in a 10% DMSO solution. Mechanical and enzymatic dissociation parameters were optimized for viable cell extraction and surface protein preservation for cell sorting and mass cytometry, as well as for reproducibility in RNA sequencing (RNA-seq). Cryopreserved synovial samples were collectively analyzed at a central processing site by a custom-designed and validated 35-marker mass cytometry panel. In parallel, each sample was flow sorted into fibroblast, T-cell, B-cell, and macrophage suspensions for bulk population RNA-seq and plate-based single-cell CEL-Seq2 RNA-seq. Results: Upon dissociation, cryopreserved synovial tissue fragments yielded a high frequency of viable cells, comparable to samples undergoing immediate processing. Optimization of synovial tissue dissociation across six clinical collection sites with ~ 30 arthroplasty and ~ 20 biopsy samples yielded a consensus digestion protocol using 100 μg/ml of Liberase™ TL enzyme preparation. This protocol yielded immune and stromal cell lineages with preserved surface markers and minimized variability across replicate RNA-seq transcriptomes. Mass cytometry analysis of cells from cryopreserved synovium distinguished diverse fibroblast phenotypes, distinct populations of memory B cells and antibody-secreting cells, and multiple CD4+ and CD8+ T-cell activation states. Bulk RNA-seq of sorted cell populations demonstrated robust separation of synovial lymphocytes, fibroblasts, and macrophages. Single-cell RNA-seq produced transcriptomes of over 1000 genes/cell, including transcripts encoding characteristic lineage markers identified. Conclusions: We have established a robust protocol to acquire viable cells from cryopreserved synovial tissue with intact transcriptomes and cell surface phenotypes. A centralized pipeline to generate multiple high-dimensional analyses of synovial tissue samples collected across a collaborative network was developed. Integrated analysis of such datasets from large patient cohorts may help define molecular heterogeneity within RA pathology and identify new therapeutic targets and biomarkers

Two multiple-imaged Z = 4.05 galaxies in the cluster-lens Abell 2390

We present the first results on the identification and study of very distant field galaxies in the core of cluster-lenses, using a selection criterium based on both lens modelling and photometric redshifts. We concentrate on two multiple-imaged sources at z=4.05 in the cluster A2390. The 2 objects presented in this paper, namely H3 and H5, were identified through lens modelling as multiple images of high-redshift sources at z>3.5. We confirm the excellent agreement between this identification and both their photometric redshifts and morphologies. Our CFHT/WHT program for a systematic redshift survey of arcs in clusters has allowed to obtain a set of spectra on 3 different images at z~4: the brightest image of H3, which redshift was already confirmed by Frye & Broadhurst (1998), and the two brightest images of H5. The later is then confirmed spectroscopically as a multiple image, giving a strong support to the lens model. The main feature in each of these spectra is a strong emission line, identified as Ly-alpha, leading to z=4.05 for both H3 and H5. The spectrophotometric properties of these galaxies are studied, in particular the degeneracy in the parameter-space defined by the SFR type, age, metallicity and reddening. H3 and H5 are intrinsically bright and clumpy sources located ~100 kpc part on the source plane, with mean metallicities compatible with a fraction of solar or even solar values. All these results seem to favour a hierarchical merging scenario, where we are actually seeing a relatively advanced step for these 2 z~4 objects, with stars forming locally and efficiently from a preenriched gas.Comment: 11 pages, A&A in press, final version sent to the Edito

Discovery and Development of a Potent and Highly Selective Small Molecule Muscarinic Acetylcholine Receptor Subtype I (mAChR 1 or M-1) Antagonist In Vitro and In Vivo Probe

This article describes the discovery and development of the first highly selective, small molecule antagonist of the muscarinic acetylcholine receptor subtype I (mAChR1 or M(1)). An M(1) functional, cell-based calcium-mobilization assay identified three distinct chemical series with initial selectivity for M(1) versus M(4). An iterative parallel synthesis approach was employed to optimize all three series in parallel, which led to the development of novel microwave-assisted chemistry and provided important take home lessons for probe development projects. Ultimately, this effort produced VU0255035, a potent (IC(50) = 130 nM) and selective (>75-fold vs. M(2)-M(5) and > 10 μM vs. a panel of 75 GPCRs, ion channels and transporters) small molecule M(1) antagonist. Further profiling demonstrated that VU0255035 was centrally penetrant (Brain(AUC)/Plasma(AUC) of 0.48) and active in vivo, rendering it acceptable as both an in vitro and in vivo MLSCN/ MLPCN probe molecule for studying and dissecting M(1) function