Subinhibitory Arsenite Concentrations Lead to Population Dispersal in Thiomonas sp.

Biofilms represent the most common microbial lifestyle, allowing the survival of microbial populations exposed to harsh environmental conditions. Here, we show that the biofilm development of a bacterial species belonging to the Thiomonas genus, frequently found in arsenic polluted sites and playing a key role in arsenic natural remediation, is markedly modified when exposed to subinhibitory doses of this toxic element. Indeed, arsenite [As(III)] exposure led to a considerable impact on biofilm maturation by strongly increasing the extracellular matrix synthesis and by promoting significant cell death and lysis within microcolonies. These events were followed by the development of complex 3D-biofilm structures and subsequently by the dispersal of remobilized cells observed inside the previously formed hollow voids. Our results demonstrate that this biofilm community responds to arsenite stress in a multimodal way, enhancing both survival and dispersal. Addressing this complex bacterial response to As(III) stress, which might be used by other microorganisms under various adverse conditions, may be essential to understand how Thiomonas strains persist in extreme environments

Toxicité ovarienne du Busulfan chez l'enfant (existe-t-il un effet dose?)

DIJON-BU Médecine Pharmacie (212312103) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

La place des parents dans la démarche palliative en pédiatrie (étude qualitative autour de la situation d'un adolescent de 17 ans atteint de maladie chronique grave)

Lorsque l enfant est atteint d une maladie limitant ou menaçant son espérance de vie, une démarche de soins palliatifs est nécessaire. Il s agit d un accompagnement spécifique, complexe et qui peut parfois durer plusieurs années. Cet accompagnement mobilise toutes les compétences techniques, organisationnelles et relationnelles de nombreux intervenants qui doivent faire équipe autour de l enfant et de sa famille. L étude qualitative d une situation clinique confirme les défis que représente, dans la pratique, la mise en oeuvre d une démarche palliative dans le cadre de la maladie chronique grave, et ses enjeux en terme de qualité de vie pour l enfant et sa famille. En regroupant les points de vue des parents et de professionnels de santé, cette étude met en évidence les enjeux de l instauration et du maintien de l alliance thérapeutique entre parents et professionnels, et ce tout au long de la maladie. Etant donnée l asymétrie de toute relation soignant-soigné, l écoute accordée aux parents est à la base de cette alliance. L écoute est également la première étape du processus d information. Une information claire et continue de la part de médecins accessibles représente un soutien fondamental pour les parents. Identifier au plus tôt les situations nécessitant une démarche palliative, c est-à-dire une démarche de soin globale, interdisciplinaire, centrée sur les besoins du patient et de sa famille, est indispensable afin d établir ensemble un projet de soins cohérent et intégré aux projets de vie de l enfant et de ses parents.DIJON-BU Médecine Pharmacie (212312103) / SudocSudocFranceF

Identification des gènes impliqués dans la formation de biofilm chez une souche atypique de Streptococcus thermophilus

International audienc

Identification des gènes impliqués dans la formation de biofilm chez une souche atypique de Streptococcus thermophilus

International audienc

Biofilm formation of the food spoiler Brochothrix thermosphacta on different industrial surface materials using a biofilm reactor

International audienc

[i]Streptococcus thermopilus[/i] biofilm lifestyle : A remnant of commensal ancestral life?

Microorganisms have a long history of use in food production and preservation. Their adaptation to food environments has profoundly modified their features, mainly through genomic flux. Streptococcus thermophilus, one of the most frequent starter culture organisms consumed daily by humans emerged recently from a commensal ancestor. As such, it is a useful model for genomic studies of bacterial domestication processes. Many streptococcal species form biofilms, a key feature of the major lifestyle of these bacteria in nature. However, few descriptions of S. thermophilus biofilms have been reported. An analysis of the ability of a representative collection of natural isolates to form biofilms revealed that S. thermophilus was a poor biofilm producer and that this characteristic was associated with an inability to attach firmly to surfaces. The identification of three biofilm-associated genes in the strain producing the most biofilms shed light on the reasons for the rarity of this trait in this species. These genes encode proteins involved in crucial stages of biofilm formation and are heterogeneously distributed between strains. One of the biofilm genes appears to have been acquired by horizontal transfer. The other two are located in loci presenting features of reductive evolution, and are absent from most of the strains analyzed. Their orthologs in commensal bacteria are involved in adhesion to host cells, suggesting that they are remnants of ancestral functions. The biofilm phenotype appears to be a commensal trait that has been lost during the genetic domestication of S. thermophilus, consistent with its adaptation to the milk environment and the selection of starter strains for dairy fermentations

Usefulness of the EMA flow cytometric test in the diagnosis of hereditary spherocytosis post-transfusion

IF 5.67International audienc

Germline JAK2 L611S mutation in a child with thrombocytosis

IF 9.090 (2017)International audienc

<i>Streptococcus thermophilus</i> Biofilm Formation: A Remnant Trait of Ancestral Commensal Life?

<div><p>Microorganisms have a long history of use in food production and preservation. Their adaptation to food environments has profoundly modified their features, mainly through genomic flux. <i>Streptococcus thermophilus</i>, one of the most frequent starter culture organisms consumed daily by humans emerged recently from a commensal ancestor. As such, it is a useful model for genomic studies of bacterial domestication processes. Many streptococcal species form biofilms, a key feature of the major lifestyle of these bacteria in nature. However, few descriptions of <i>S</i>. <i>thermophilus</i> biofilms have been reported. An analysis of the ability of a representative collection of natural isolates to form biofilms revealed that <i>S</i>. <i>thermophilus</i> was a poor biofilm producer and that this characteristic was associated with an inability to attach firmly to surfaces. The identification of three biofilm-associated genes in the strain producing the most biofilms shed light on the reasons for the rarity of this trait in this species. These genes encode proteins involved in crucial stages of biofilm formation and are heterogeneously distributed between strains. One of the biofilm genes appears to have been acquired by horizontal transfer. The other two are located in loci presenting features of reductive evolution, and are absent from most of the strains analyzed. Their orthologs in commensal bacteria are involved in adhesion to host cells, suggesting that they are remnants of ancestral functions. The biofilm phenotype appears to be a commensal trait that has been lost during the genetic domestication of <i>S</i>. <i>thermophilus</i>, consistent with its adaptation to the milk environment and the selection of starter strains for dairy fermentations.</p></div