2006_and_2007_YP_Chem_Gen_data

Microsatellite and otolith microchemical data for larval and juvenile yellow perch from 2006 and 200

2007collectionsites

Collection site information for larval yellow perch during 200

Number of genotypes (N), alleles (N_A), and observed (H_O) and expected (H_E) heterozygosity for the 12 microsatellite loci (Li et al. 2007) used to genotype larval yellow perch (YP) collected in north-shore (NS) and south-shore (SS) waters of Lake Erie’s western basin during 2006 (N_{NS larvae} = 151, N_{SS larvae} = 91) and 2007 (N_{NS larvae} = 283, N_{NS larvae} = 81).

<p>Groups are denoted by collection location (NS, SS) followed by the year of collection (06, 07). Note: Data in bold denotes deviations from HWE (following Bonferroni correction).</p><p>Number of genotypes (N), alleles (N_A), and observed (H_O) and expected (H_E) heterozygosity for the 12 microsatellite loci (Li et al. 2007) used to genotype larval yellow perch (YP) collected in north-shore (NS) and south-shore (SS) waters of Lake Erie’s western basin during 2006 (N_{NS larvae} = 151, N_{SS larvae} = 91) and 2007 (N_{NS larvae} = 283, N_{NS larvae} = 81).</p

F_ST values between larvae collected in north-shore (NS) and south-shore (SS) waters of western Lake Erie during 2006 and 2007 based on seven microsatellite loci, with all larvae included (top) and only larvae < 8 mm total length included (i.e., those most likely to be passively dispersed; bottom).

<p>Sample sizes (n) are included. Rows indicate confidence in hatch-location assignment: None: null assignments based on capture location; Best: assigned to single most likely hatching location (SS or NS) based on backtracking; 60%: assigned to hatching location with at least 60% confidence; 70%: assigned to hatching location with at least 70% confidence; 80%: assigned to hatching location with at least 80% confidence; 90%: assigned to hatching location with at least 90% confidence.</p><p>F_ST values between larvae collected in north-shore (NS) and south-shore (SS) waters of western Lake Erie during 2006 and 2007 based on seven microsatellite loci, with all larvae included (top) and only larvae < 8 mm total length included (i.e., those most likely to be passively dispersed; bottom).</p

Predicted origins of juvenile yellow perch collected in open waters of western Lake Erie using otolith microchemistry from larvae collected in north-shore (NS) and south-shore (SS) water during a) 2006 and b) 2007.

<p>A total of N = 98 juveniles were analyzed each year. Juveniles with < 70% likelihood of originating within a population were consider “failed” and juveniles with a probability > 70% were assigned to NS or SS. Certainty in hatching locations of <i>larvae</i> used to develop classification functions was as follows: None = null assignments based on capture location (i.e., no backtracking used); Best = larvae assigned to single most likely hatching location (SS or NS) based on backtracking; and 60, 70, 80, 90 = larvae assigned to hatching location after backtracking revision with 60, 70, 80, 90% levels of certainty in hatching origin, respectively.</p

Larval yellow perch self-assignment results using a Random Forest (RF), Linear Discriminant Function Analysis (LDA), Quadratic Discriminant Function Analysis (QDA), and Neural Network (NN) based on otolith microchemistry data for a) 2006 and b) 2007.

<p>A total of N = 47 and N = 71 larvae were analyzed in 2006 and 2007. Confidence in hatching locations is as follows: None = null assignments based on capture location (i.e., no backtracking used); Best = larvae assigned to single most likely hatching location (SS or NS) based on backtracking; and 60, 70, 80, 90 = larvae assigned to hatching location after backtracking revision with 60, 70, 80, 90% levels of certainty in hatching origin, respectively.</p

Predicted origins of juvenile yellow perch collected in open waters of western Lake Erie using microsatellites (seven loci) from larvae collected during 2006 (a: all larvae; b: only larvae < 8 mm total length, TL) and 2007 (c: all larvae; d: only larvae < 8 mm TL).

<p>A total of N = 119 and N = 167 juveniles were analyzed during 2006 and 2007. Juveniles with < 30% likelihood of originating within a population were “excluded” (i.e., they were not included in the analysis); juveniles with a 30 to 70% likelihood were considered “failed,” (i.e., we had little confidence in their hatching-location assignment); and juveniles with a probability > 70% were assigned to the NS or SS breeding population with high confidence. Certainty in hatching locations of <i>larvae</i> used to develop classification functions was as follows: None = null assignments based on capture location (i.e., no backtracking used); Best = larvae assigned to single most likely hatching location (SS or NS) based on backtracking; and 60, 70, 80, 90 = larvae assigned to hatching location after backtracking revision with 60, 70, 80, 90% levels of certainty in hatching origin, respectively.</p

Summary of the percentage of individuals correctly self-assigned (larvae) or successfully classified to a natal group (juveniles) without and with backtracking revision of initial larval assignments.

<p>The backtracking column includes only the data using the 90% confidence threshold for larval hatching location assignment. The data also include only larvae < 8 mm TL.</p><p>Summary of the percentage of individuals correctly self-assigned (larvae) or successfully classified to a natal group (juveniles) without and with backtracking revision of initial larval assignments.</p

Self-assignment results based on microsatellite DNA data (seven loci) for larval yellow perch collected in Lake Erie’s western basin during a) 2006 and b) 2007.

<p>For sample sizes, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120752#pone.0120752.t002" target="_blank">Table 2</a>. Confidence in hatching locations is as follows: None = null assignments based on capture location (i.e., no backtracking used); Best = larvae assigned to single most likely hatching location (SS or NS) based on backtracking; and 60, 70, 80, 90 = larvae assigned to hatching location after backtracking revision with 60, 70, 80, 90% levels of certainty in hatching origin, respectively.</p

Simultaneous Release of Fe and As during the Reductive Dissolution of Pb–As Jarosite by <i>Shewanella putrefaciens</i> CN32

Jarosites are produced during metallurgical processing, on oxidized sulfide deposits, and in acid mine drainage environments. Despite the environmental relevance of jarosites, few studies have examined their biogeochemical stability. This study demonstrates the simultaneous reduction of structural Fe­(III) and aqueous As­(V) during the dissolution of synthetic Pb–As jarosite (PbFe₃(SO₄,AsO₄)₂(OH)₆) by <i>Shewanella putrefaciens</i> using batch experiments under anaerobic circumneutral conditions. Fe­(III) reduction occurred immediately in inoculated samples while As­(V) reduction was observed after 72 h. XANES spectra showed As­(III) (14.7%) in the solid phase at 168 h coincident with decreased aqueous As­(V). At 336 h, XANES spectra and aqueous speciation analysis demonstrated 20.2% and 3.0% of total As was present as As­(III) in the solid and aqueous phase, respectively. In contrast, 12.4% of total Fe was present as aqueous Fe­(II) and was below the detection limits of XANES in the solid phase. TEM-EDS analysis at 336 h showed secondary precipitates enriched in Fe and O with minor amounts of As and Pb. Based on experimental data and thermodynamic modeling, we suggest that structural Fe­(III) reduction was thermodynamically driven while aqueous As­(V) reduction was triggered by detoxification induced to offset the high As­(V) (328 μM) concentrations released during dissolution