5 research outputs found
Genome, proteome and structure of a T7-like bacteriophage of the kiwifruit canker phytopathogen pseudomonas syringae pv. actinidiae
La pseudomonas syringae pv. actinidiae es un patógeno responsable significativo de la afta bacteriana severa del kiwi (Actinidia sp.). Los bacteriófagos infectados de este fitopatógeno tienen potencial como agentes de control biológico como parte de un enfoque integrado de la gestión del cancro bacteriano, y para su uso como herramientas molecular para el estudio de esta bacteria. Una variedad de bacteriófagos fueron previamente aislados, antes de ser infectados con P. syringae pv. Actinidiae; y sus propiedades básicas fueron caracterizadas para proporcionar un marco para la formulación de estos fagos, como agentes de biocontrol. Aquí, hemos examinado con más detalle el φPsa17, un fago con la capacidad de infectar a una amplia gama de cepas P. syringae pv. Actinidiae, único miembro de la Podoviridae en esta colección. La morfología de partículas fue visualizada mediante criomicroscopía electrónica, el genoma fue secuenciado, y sus proteínas estructurales fueron analizados usando shotgun proteomics. Estos estudios demostraron que 40,525 φPsa17 tiene un genoma de BP, es un miembro de género T7likevirus y está estrechamente relacionada con la pseudomonada llamada fágicas φPSA2 y GH-1. Once proteínas estructurales (andamios) fueron detectados por la proteómica y φPsa17 tiene una cápside de aproximadamente 60 nm de diámetro. No fueron identificados genes indicativos de un ciclo de vida lisogénica, sugiriendo que el fago es necesariamente lítico. Estas características indican que φPsa17 pueden ser adecuadas para la formulación como un agente de biocontrol de P. syringae pv. actinidiaePseudomonas syringae pv. actinidiae is an economically significant pathogen responsible for severe bacterial canker of kiwifruit (Actinidia sp.). Bacteriophages infecting this phytopathogen have potential as biocontrol agents as part of an integrated approach to the management of bacterial canker, and for use as molecular tools to study this bacterium. A variety of bacteriophages were previously isolated that infect P. syringae pv. actinidiae, and their basic properties were characterized to provide a framework for formulation of these phages as biocontrol agents. Here, we have examined in more detail φPsa17, a phage with the capacity to infect a broad range of P. syringae pv. actinidiae strains and the only member of the Podoviridae in this collection. Particle morphology was visualized using cryo-electron microscopy, the genome was sequenced, and its structural proteins were analysed using shotgun proteomics. These studies demonstrated that φPsa17 has a 40,525 bp genome, is a member of the T7likevirus genus and is closely related to the pseudomonad phages φPSA2 and gh-1. Eleven structural proteins (one scaffolding) were detected by proteomics and φPsa17 has a capsid of approximately 60 nm in diameter. No genes indicative of a lysogenic lifecycle were identified, suggesting the phage is obligately lytic. These features indicate that φPsa17 may be suitable for formulation as a biocontrol agent of P. syringae pv. actinidiaeTrabajo patrocinado por.
Royal Society. Fellowship Rutherford, para Peter C. Fineran
Otago School of Medical Sciences Summer Research Scholarship, para Danni ChenpeerReviewe
Transfer cells and flange cells in sinkers of the mistletoePhoradendron macrophyllum (Viscaceae), and their novel combination
Cyto- and histochemical demonstration of lignins in plant cell walls: an evaluation of the chlorine water/ethanolamine-silver nitrate method of Coppick and Fowler
Recommended from our members
CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes
CRISPR-Cas enzymes enable RNA-guided bacterial immunity and are widely used for biotechnological applications including genome editing. In particular, the Class 2 CRISPR-associated enzymes (Cas9, Cas12 and Cas13 families), have been deployed for numerous research, clinical and agricultural applications. However, the immense genetic and biochemical diversity of these proteins in the public domain poses a barrier for researchers seeking to leverage their activities. We present CasPEDIA (http://caspedia.org), the Cas Protein Effector Database of Information and Assessment, a curated encyclopedia that integrates enzymatic classification for hundreds of different Cas enzymes across 27 phylogenetic groups spanning the Cas9, Cas12 and Cas13 families, as well as evolutionarily related IscB and TnpB proteins. All enzymes in CasPEDIA were annotated with a standard workflow based on their primary nuclease activity, target requirements and guide-RNA design constraints. Our functional classification scheme, CasID, is described alongside current phylogenetic classification, allowing users to search related orthologs by enzymatic function and sequence similarity. CasPEDIA is a comprehensive data portal that summarizes and contextualizes enzymatic properties of widely used Cas enzymes, equipping users with valuable resources to foster biotechnological development. CasPEDIA complements phylogenetic Cas nomenclature and enables researchers to leverage the multi-faceted nucleic-acid targeting rules of diverse Class 2 Cas enzymes