2,708 research outputs found
Phase Gate Implementation of Project Definition Rating Index (PDRI 9f AF MILCON Project Development: A Comprehensive Analysis
The $1.3B Air Force Military Construction (MILCON) program has shown a trend of 6% cost growth and 12% schedule growth in the construction phases of its projects. Air Force leadership has attributed this to a shortfall in the standardized processes for requirements definition and scope development. The Project Definition Rating Index (PDRI) has been in use on Air Force MILCON projects for almost a decade, but the development and approval process for these projects does not lend itself to direct PDRI implementation. 96 PDRI MILCON projects executed from 2009 to 2016 were analyzed for trends in project development implementation strategies using Analysis of Variance and Regression. It is suggested that the Air Force provides clear, mandated guidance for earlier project development and improve data collection, storage, and analysis methods to aid additional research efforts
Activated Ion Electron Capture Dissociation (AI ECD) of proteins: synchronization of infrared and electron irradiation with ion magnetron motion.
Here, we show that to perform activated ion electron capture dissociation (AI-ECD) in a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer equipped with a CO(2) laser, it is necessary to synchronize both infrared irradiation and electron capture dissociation with ion magnetron motion. This requirement is essential for instruments in which the infrared laser is angled off-axis, such as the Thermo Finnigan LTQ FT. Generally, the electron irradiation time required for proteins is much shorter (ms) than that required for peptides (tens of ms), and the modulation of ECD, AI ECD, and infrared multiphoton dissociation (IRMPD) with ion magnetron motion is more pronounced. We have optimized AI ECD for ubiquitin, cytochrome c, and myoglobin; however the results can be extended to other proteins. We demonstrate that pre-ECD and post-ECD activation are physically different and display different kinetics. We also demonstrate how, by use of appropriate AI ECD time sequences and normalization, the kinetics of protein gas-phase refolding can be deconvoluted from the diffusion of the ion cloud and measured on the time scale longer than the period of ion magnetron motion
Topoisomer Differentiation of Molecular Knots by FTICR MS: Lessons from Class II Lasso Peptides
Lasso peptides constitute a class of bioactive peptides sharing a knotted
structure where the C-terminal tail of the peptide is threaded through and
trapped within an N-terminalmacrolactamring. The structural characterization of
lasso structures and differentiation from their unthreaded topoisomers is not
trivial and generally requires the use of complementary biochemical and
spectroscopic methods. Here we investigated two antimicrobial peptides
belonging to the class II lasso peptide family and their corresponding
unthreaded topoisomers: microcin J25 (MccJ25), which is known to yield
two-peptide product ions specific of the lasso structure under collisioninduced
dissociation (CID), and capistruin, for which CID does not permit to
unambiguously assign the lasso structure. The two pairs of topoisomers were
analyzed by electrospray ionization Fourier transform ion cyclotron resonance
mass spectrometry (ESI-FTICR MS) upon CID, infrared multiple photon
dissociation (IRMPD), and electron capture dissociation (ECD). CID and
ECDspectra clearly permitted to differentiate MccJ25 from its non-lasso
topoisomer MccJ25-Icm, while for capistruin, only ECD was informative and
showed different extent of hydrogen migration (formation of c\bullet/z from
c/z\bullet) for the threaded and unthreaded topoisomers. The ECD spectra of the
triply-charged MccJ25 and MccJ25-lcm showed a series of radical b-type product
ions {\eth}b0In{\TH}. We proposed that these ions are specific of
cyclic-branched peptides and result from a dual c/z\bullet and y/b
dissociation, in the ring and in the tail, respectively. This work shows the
potentiality of ECD for structural characterization of peptide topoisomers, as
well as the effect of conformation on hydrogen migration subsequent to electron
capture
Water Stress Affects Development Time but Not Takeoff Performance in the Butterfly Pararge aegeria
Most organisms are limited in the amount and type of resources they are able to extract from the environment. The juvenile environment is particularly important in this regard, as conditions over ontogeny can influence the adult phenotype. Whole-organism performance traits, such as locomotion, are susceptible to such environmental effects, yet the specific biotic and abiotic factors driving performance plasticity have received little attention. We tested whether speckled wood Pararge aegeria L. butterflies reared under conditions of water stress exhibited poorer flight morphology and performance than control individuals. Despite large differences in mortality between treatments, we found no effects of water stress treatment on takeoff performance and only minor treatment effects on flight morphology. However, butterflies reared on water-stressed diets exhibited both significantly greater mortality and longer development times than did control individuals. Pararge aegeria larvae may compensate for this stress by prolonging development, resulting in similar realized performance capacities at least in takeoff performance in surviving adult butterflies; other measures of flight performance remain to be considered. Alternatively, the adult phenotype may be insulated from environmental effects at the larval stage in these insects
The Role of the D13 (1520) Resonance in eta Electroproduction
We investigate the electroproduction of eta mesons below a center of momentum
energy of 1.6 GeV, with particular emphasis on the roles of the N*(1535) and
N*(1520) resonances. Using the effective Lagrangian approach, we show that the
transverse helicity amplitude of the N*(1535) can be extracted with good
accuracy from the new eta electroproduction data, under reasonable assumptions
for the strength of the longitudinal helicity amplitude. In addition, although
the differential cross section is found to to have a small sensitivity to the
N*(1520) resonance, it is shown that a recently completed double polarization
experiment is very sensitive to this resonance.Comment: 7 pages, Revtex, 3 figure
Ancient expansion of the Hox cluster in Lepidoptera generated four homeobox genes implicated in extra-embryonic tissue formation
Gene duplications within the conserved Hox cluster are rare in animal evolution, but in Lepidoptera an array of divergent Hox-related genes (Shx genes) has been reported between pb and zen. Here, we use genome sequencing of five lepidopteran species (Polygonia c-album, Pararge aegeria, Callimorpha dominula, Cameraria ohridella, Hepialus sylvina) plus a caddisfly outgroup (Glyphotaelius pellucidus) to trace the evolution of the lepidopteran Shx genes. We demonstrate that Shx genes originated by tandem duplication of zen early in the evolution of large clade Ditrysia; Shx are not found in a caddisfly and a member of the basally diverging Hepialidae (swift moths). Four distinct Shx genes were generated early in ditrysian evolution, and were stably retained in all descendent Lepidoptera except the silkmoth which has additional duplications. Despite extensive sequence divergence, molecular modelling indicates that all four Shx genes have the potential to encode stable homeodomains. The four Shx genes have distinct spatiotemporal expression patterns in early development of the Speckled Wood butterfly (Pararge aegeria), with ShxC demarcating the future sites of extraembryonic tissue formation via strikingly localised maternal RNA in the oocyte. All four genes are also expressed in presumptive serosal cells, prior to the onset of zen expression. Lepidopteran Shx genes represent an unusual example of Hox cluster expansion and integration of novel genes into ancient developmental regulatory networks
Threshold eta and eta' electroproduction off nucleons
The electroproduction of eta and eta' mesons on the proton and the neutron is
investigated at tree level within the framework of U(3) chiral perturbation
theory. In addition to the Born terms low-lying resonances such as the vector
mesons and J^P= 1/2^+, 1/2^- baryon resonances are included explicitly and
their contributions are calculated. Results for the separated differential
cross sections are presented.Comment: 24 pages, 7 figure
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