Detecção do virus da anemia das galinhas em coinfecção com o vírus doença infecciosa bursal em frangos

Este estudo investigou a manifestação do vírus da Anemia Infecciosa das Aves (VAIA) em lotes de frangos que apresentavam retardo no crescimento e aumento da mortalidade observado a partir do quarto dia de idade. Clinicamente, as aves apresentavam depresão, palidez, despigmentação e retardo de crescimento. À necropsia, as aves apresentavam lesões compatíveis com a infecção pelo vírus da Anemia infecciosa das aves (VAIA). Amostras de fígado, baço e timo foram examinadas por PCR que amplifica um frangmento de 675 pb do gene VP-1 do VAIA. Todos os órgãos examinados foram positivos para o vírus da Anemia Infecciosa das Aves. Os demais patógenos, como adenovírus, reovírus, astrovírus, vírus da doença infecciosa bursal e coronavírus aviário não foram detectados pelas diferentes técnicas laboratoriais, como sorologia, PCR ou PAGE. Os resultados mostraram que o vírus da Anemia Infecciosa das Aves (VAIA) pode manifestar-se clinicamente nos primeiros dias de vida dos frangos - um fato ainda não reportado - associado ao vírus vacinal da doença infecciosa bursal (DIB) cepa forte pode induzir um persistente retardo de crescimento, por várias semanas, em frangos.This survey aimed to investigate chicken anemia virus (CAV) in broilers flocks experimenting retarded growth and increasing mortality since the fourth day of age. Clinically, chickens presented depression, paleness, depigmentation and retarded growth. At necropsy, chickens presented CAV-compatible lesions. Samples from liver, spleen and thymus were tested by PCR for a 675-bp fragment of the CAV VP-1 gene, and all tested samples were positive. Serological and molecular techniques did not detect other pathogens, such as adenovirus, reovirus, astrovirus, infectious bursal disease and avian infectious bronchitis virus. These results showed that chicken anemia virus (CAV) may occur since the first few days of life in broilers - a fact not as yet reported -, associated with high pathogenic Infectious Bursal Disease Virus (IBDV) vaccine strain may induce a persistent growth retarded for several weeks in broilers

Prealignment B(E2)-anomaly in 124Xe

Lifetimes of 17 levels of 124Xe were measured using the Recoil Distance Doppler Shift method. An anomalous E2-reduction was observed, starting at Iπ = 8+ and Iπ = 5+ in the groundstate and in the quasi-gamma band respectively. This anomaly is discussed in terms of the Interacting Boson Model

Proof of Concept for Levodopa Treatment in Rescuing Retinal Morphology and Visual Function in Albinism (Video)

There is a paucity of treatments for oculocutaneous albinism (OCA), a condition characterised by pigment deficiency, abnormal retinal development, and significant visual disability. L-DOPA, a signalling molecule which is essential for normal retinal development, is deficient in OCA. Residual plasticity of the developing retina in young children with albinism has been demonstrated, suggesting a post-natal window for therapeutic rescue. In this study, we investigate if post-natal retinal morphology and visual function in OCA can be improved through oral Levodopa supplementation, if administered during the critical period of neuroplasticity

Proof of Concept for Levodopa Treatment in Rescuing Retinal Morphology and Visual Function in Albinism (PDF)

There is a paucity of treatments for oculocutaneous albinism (OCA), a condition characterised by pigment deficiency, abnormal retinal development, and significant visual disability. L-DOPA, a signalling molecule which is essential for normal retinal development, is deficient in OCA. Residual plasticity of the developing retina in young children with albinism has been demonstrated, suggesting a post-natal window for therapeutic rescue. In this study, we investigate if post-natal retinal morphology and visual function in OCA can be improved through oral Levodopa supplementation, if administered during the critical period of neuroplasticity

Proof of Concept for Levodopa Treatment in Rescuing Retinal Morphology and Visual Function in Albinism (Slides)

There is a paucity of treatments for oculocutaneous albinism (OCA), a condition characterised by pigment deficiency, abnormal retinal development, and significant visual disability. L-DOPA, a signalling molecule which is essential for normal retinal development, is deficient in OCA. Residual plasticity of the developing retina in young children with albinism has been demonstrated, suggesting a post-natal window for therapeutic rescue. In this study, we investigate if post-natal retinal morphology and visual function in OCA can be improved through oral Levodopa supplementation, if administered during the critical period of neuroplasticity

Brain Mapping of Ghrelin O-Acyltransferase in Goldfish (Carassius Auratus): Novel Roles for the Ghrelinergic System in Fish?

Ghrelin O-acyltransferase (GOAT) is the enzyme responsible for acylation of ghrelin, a gut-brain hormone with important roles in many physiological functions in vertebrates. Many aspects of GOAT remain to be elucidated, especially in fish, and particularly its anatomical distribution within the different brain areas has never been reported to date. The present study aimed to characterize the brain mapping of GOAT using RT-qPCR and immunohistochemistry in a teleost, the goldfish (Carassius auratus). Results show that goat transcripts are expressed in different brain areas of the goldfish, with the highest levels in the vagal lobe. Using immunohistochemistry, we also report the presence of GOAT immunoreactive cells in different encephalic areas, including the telencephalon, some hypothalamic nuclei, pineal gland, optic tectum and cerebellum, although they are especially abundant in the hindbrain. Particularly, an important signal is observed in the vagal lobe and some fiber tracts of the brainstem, such as the medial longitudinal fasciculus, Mauthneri fasciculus, secondary gustatory tract and spinothalamic tract. Most of the forebrain areas where GOAT is detected, particularly the hypothalamic nuclei, also express the ghs-r1a ghrelin receptor and other appetite-regulating hormones (e.g., orexin and NPY), supporting the role of ghrelin as a modulator of food intake and energy balance in fish. Present results are the first report on the presence of GOAT in the brain using imaging techniques. The high presence of GOAT in the hindbrain is a novelty, and point to possible new functions for the ghrelinergic system in fish