remote sensing supports the definition of the water quality status of lake omodeo italy

AbstractLake Omodeo is the largest artificial reservoir of Sardinia and its waters are a valuable resource for irrigation, domestic and industrial purposes. Lake Omodeo has serious problems of eutrophication. Since 2007 the local water authority has been undertaken a monitoring program designed to test an integrated methodology based on field measurements and remote sensing. This study illustrates the production of multitemporal spatialised maps of chlorophyll-a concentrations from satellite data acquired from Medium Resolution Imaging Spectrometer (MERIS). The analysis confirmed the eutrophic status of Omodeo. especially between spring and summer (mainly due to cyanobacteria bloom) assessing their dependency on weather conditions and river inputs

Osservazione delle zone marine costiere da dati ottici multi-sensore: il caso studio della foce del Volturno

Questo lavoro s’inserisce nell’ambito del progetto bandiera RITMARE (Ricerca ITaliana per il MARE, 2012-2016, MIUR-CNR) che ha lo scopo di studiare, monitorare, gestire e valorizzare la risorsa marina in Italia. A tale fine il telerilevamento è parte integrante al progetto che, in questo studio, viene applicato alla fascia costiera della foce del Volturno. Lo studio si avvale dell’utilizzo di immagini satellitari (RapidEye (2013) e Landsat-8 OLI (2014)) e aviotrasportate (MIVIS (2011)). A supporto dell’analisi delle immagini sono state acquisite misure in-situ di riflettanza e di concentrazione dei seguenti parametri: clorofilla-a (Chl-a), sedimenti totali sospesi (TSM) e sostanza organica colorata disciolta (CDOM) durante una campagna svoltasi in agosto 2014. I dati telerilevati, geocodificati e co-registrati, sono stati pre-elaborati per correggere gli effetti radiometrici ed atmosferici. Le immagini sono state successivamente elaborate per classificare le acque marino-costiere secondo diversi gradi di complessità ottica e per produrre mappe di concentrazione di Chl-a, TSM e CDOM. In particolare, per la generazione delle mappe è stato applicato un approccio semi-empirico basato su analisi di correlazione dei dati in-situ; in particolare tra valori spettrali di riflettanza (es. rapporti di banda) e le corrispondenti misure di concentrazione di Chl-a, TSM e CDOM. I risultati mostrano un gradiente crescente di complessità ottica delle acque spostandosi dalle zone pelagiche verso le aree costiere. In queste aree l’analisi del dato OLI ha mostrato una variabilità spaziale di Chl-a, TSM e CDOM imputabile ai regimi idrologici del fiume Volturno

multitemporal analysis of algal blooms with meris images in a deep meromictic lake

MERIS images (2003-2011) were used to detect algal bloom events in Lake Idro (Northern Italy) applying a semi-empirical algorithm. From the study of an intense phenomenon occurred in late summer 2010, a retrospective analysis of similar events during late summer/ early autumn period was performed. High intra- and inter-annual variability was observed and three additional bloom events were identified on 2003, 2005 and 2008. Hydrological and weather parameters were examined at different temporal intervals (August-October, September-October and monthly from August to October) to investigate the regulating factors of bloom incidence. Rather low temperatures and the persistence of clouds seem t

Crucial role of zebrafish prox1 in hypothalamic catecholaminergic neurons development

<p>Abstract</p> <p>Background</p> <p><it>Prox1</it>, the vertebrate homolog of <it>prospero </it>in <it>Drosophila melanogaster</it>, is a divergent homeogene that regulates cell proliferation, fate determination and differentiation during vertebrate embryonic development.</p> <p>Results</p> <p>Here we report that, in zebrafish, <it>prox1 </it>is widely expressed in several districts of the Central Nervous System (CNS). Specifically, we evidenced <it>prox1 </it>expression in a group of neurons, already positive for <it>otp1</it>, located in the hypothalamus at the level of the posterior tuberculum (PT). Prox1 knock-down determines the severe loss of hypothalamic catecholaminergic (CA) neurons, identified by tyrosine hydroxylase (TH) expression, and the synergistic <it>prox1/otp1 </it>overexpression induces the appearance of hypothalamic supernumerary TH-positive neurons and ectopic TH-positive cells on the yolk epitelium.</p> <p>Conclusion</p> <p>Our findings indicate that <it>prox1 </it>activity is crucial for the proper development of the <it>otp1</it>-positive hypothalamic neuronal precursors to their terminal CA phenotype.</p

Measuring freshwater aquatic ecosystems: The need for a hyperspectral global mapping satellite mission

AbstractFreshwater ecosystems underpin global water and food security, yet are some of the most endangered ecosystems in the world because they are particularly vulnerable to land management change and climate variability. The US National Research Council's guidance to NASA regarding missions for the coming decade includes a polar orbiting, global mapping hyperspectral satellite remote sensing mission, the Hyperspectral Infrared Imager (HyspIRI), to make quantitative measurements of ecosystem change. Traditionally, freshwater ecosystems have been challenging to measure with satellite remote sensing because they are small and spatially complex, require high fidelity spectroradiometry, and are best described with biophysical variables derived from high spectral resolution data. In this study, we evaluate the contribution of a hyperspectral global mapping satellite mission to measuring freshwater ecosystems. We demonstrate the need for such a mission, and evaluate the suitability and gaps, through an examination of the measurement resolution issues impacting freshwater ecosystem measurements (spatial, temporal, spectral and radiometric). These are exemplified through three case studies that use remote sensing to characterize a component of freshwater ecosystems that drive primary productivity. The high radiometric quality proposed for the HyspIRI mission makes it uniquely well designed for measuring freshwater ecosystems accurately at moderate to high spatial resolutions. The spatial and spectral resolutions of the HyspIRI mission are well suited for the retrieval of multiple biophysical variables, such as phycocyanin and chlorophyll-a. The effective temporal resolution is suitable for characterizing growing season wetland phenology in temperate regions, but may not be appropriate for tracking algal bloom dynamics, or ecosystem responses to extreme events in monsoonal regions. Global mapping missions provide the systematic, repeated measurements necessary to measure the drivers of freshwater biodiversity change. Archival global mapping missions with open access and free data policies increase end user uptake globally. Overall, an archival, hyperspectral global mapping mission uniquely meets the measurement requirements of multiple end users for freshwater ecosystem science and management

Zebrafish Numb and Numblike Are Involved in Primitive Erythrocyte Differentiation

BACKGROUND:Notch signaling is an evolutionarily conserved regulatory circuitry implicated in cell fate determination in various developmental processes including hematopoietic stem cell self-renewal and differentiation of blood lineages. Known endogenous inhibitors of Notch activity are Numb-Nb and Numblike-Nbl, which play partially redundant functions in specifying and maintaining neuronal differentiation. Nb and Nbl are expressed in most tissues including embryonic and adult hematopoietic tissues in mice and humans, suggesting possible roles for these proteins in hematopoiesis. METHODOLOGY AND PRINCIPAL FINDINGS:We employed zebrafish to investigate the possible functional role of Numb and Numblike during hematopoiesis, as this system allows a detailed analysis even in embryos with severe defects that would be lethal in other organisms. Here we describe that nb/nbl knockdown results in severe reduction or absence of embryonic erythrocytes in zebrafish. Interestingly, nb/nbl knocked-down embryos present severe downregulation of the erythroid transcription factor gata1. This results in erythroblasts which fail to mature and undergo apoptosis. Our results indicate that Notch activity is increased in embryos injected with nb/nbl morpholino, and we show that inhibition of Notch activation can partially rescue the hematopoietic phenotype. CONCLUSIONS AND SIGNIFICANCE:Our results provide the first in vivo evidence of an involvement of Numb and Numblike in zebrafish erythroid differentiation during primitive hematopoiesis. Furthermore, we found that, at least in part, the nb/nbl morphant phenotype is due to enhanced Notch activation within hematopoietic districts, which in turn results in primitive erythroid differentiation defects

An efficient dissociation protocol for generation of single cell suspension from zebrafish embryos and larvae

Zebrafish (Danio rerio) has emerged as a powerful animal model to study developmental processes and human diseases. The introduction of CRISPR/Cas9 as a genome editing tool allowed the generation of genetic mutants with high-throughput (Varshney et al., 2015) and has opened the possibility to understand gene function not only during embryonic stages but also in larval stages. Therefore, there is an increasing need to optimize methods for embryo and larvae dissociation that allow the generation of single cell suspension for fluorescence-activated cell sorting (FACS), RNA extraction and single cell RNA-sequencing. Method name: Zebrafish embryo, Larvae dissociation to single cell suspension, Keywords: Zebrafish, Dissociation, Single cell suspensio

Use of Satellite and In Situ Reflectance Data for Lake Water Color Characterization in the Everest Himalayan Region

This study applied remote sensing techniques to the study of water color in Himalayan glacial lakes as a proxy of suspended solid load. In situ measurements gathered in 5 lakes in October 2014 during satellite data acquisition enabled the characterization of water reflectance and clarity and supported image processing. Field data analysis led to a distinction between 3 water colors and a consequent lake water color classification on a regional scale from Landsat-8 data previously corrected for atmospheric and adjacency effects. Several morphometric parameters (lake size and shape, distance between lake and glacier) were also computed for the lakes thus classified. The results showed spatial and temporal variations in lake water color, suggestive of relationships between glacier shrinkage and the presence of brighter and more turbid water. A finer-scale analysis of the spatial variability of water reflectance on Chola Lake (based on GeoEye-1 data captured on 18 October 2014) showed the contribution of water component absorption from the inflow. Overall, the findings support further research to monitor Himalayan lakes using both Landsat-8 and Sentinel-2 (with its improved resolutions)

A Satellite-Based Tool for Mapping Evaporation in Inland Water Bodies: Formulation, Application, and Operational Aspects

With the increase of evaporation projected for water bodies worldwide, there is a growing need for flexible and low data-demanding tools enabling the monitoring and management of water resources. This study presents a simple satellite-based tool named LakeVap specifically designed for mapping evaporation from lakes and reservoirs. LakeVap requires a small amount of potentially available data with a global coverage. The tool follows a Dalton-type approach and produces instantaneous (i.e., hourly) and daily evaporation maps from satellite-derived Lake Surface Water Temperature (LSWT) maps and single-point/gridded meteorological data. The model is tested on Lake Garda, Italy, by using a long time series of LSWT (ESA CCI-Lakes) and different sources of meteorological forcing. The accuracy of LakeVap evaporation outputs is checked by comparison with those from a hydro-thermodynamic model (Delft3D) specifically set up and validated for the case study. Results are consistent and sensitive to the representativeness of the meteorological forcing. In the test site, wind speed is found to be the most spatially variable parameter, and it is significantly underestimated by the ERA5 meteorological dataset (up to 100%). The potential application of LakeVap to other case studies and in operational contexts is discussed