Synthèse de prodrogues d'oligonucléotides et étude de leur métabolisation en milieux biologiques suivie par spectrométrie de masse MALDI-TOF. Synthèse de pro-oligonucléotides N-acétylés

MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Multipurpose high-throughput filtering microarrays (HiFi) for DNA and protein assays

International audienceWe are reporting here a low cost colorimetric device for high-throughput multiplexed blood group genotyping and allergy diagnosis, displayed as an automated 96-well microtiter plate format. A porous polymeric membrane sealed at the bottom of each well accounts for the sensor support. For each sensing unit, a 6 x 6 matrix of specific probes is spotted on the external surface of the membrane resulting in 5 mm(2) microarrays. Thanks to the membrane porosity, reagents dispensed into the well can be eliminated through vacuum soaking. This unusual design drastically reduces the assay background signal. The system was first validated on robust models composed of either two complementary oligonucleotide sequences or one allergen/specific rabbit IgG pair. The quality of both oligonucleotide and protein immobilisation on the membrane substrate was then demonstrated together with the capacity to use the arrayed biomolecules as probes for the quantitative detection of specific targets (respectively complementary oligonucleotide and specific antibody). On the basis of these good results, two multiplex assays were developed for crude biological samples testing, focussing on two human in vitro diagnosis applications: a hybridisation assay for multiplex blood group genotyping and a multiparametric immunoassay for allergy diagnosis. In both cases, the transfer to crude biological samples testing was successful i.e. high signal to noise ratio of the stained membranes, reproducibility and good correlation with results obtained using routine testing procedures. (C) 2010 Elsevier B.V. All rights reserved

Multiplexed immunoassay for the rapid detection of anti-tumor-associated antigens antibodies

International audienc

A putative CA2+ and calmodulin dependent protein kinase required for bacterial and fungal symbioses

30 ref. www.sciencemag.org/cgi/content/full/1093038/DC1International audienc

An Innovative Multiplexed And Flexible Molecular Approach For The Differential Detection Of Arboviruses

International audienceNucleic acid testing during the preseroconversion viremic phase is required to differentially diagnose arboviral infections. The continuing emergence of arboviruses, such as Zika virus (ZIKV), dengue virus (DENV), and chikungunya virus (CHIKV), necessitates the development of a flexible diagnostic approach. Similar clinical signs and the priority to protect pregnant women from ZIKV infection indicate that the differential diagnosis of arboviruses is essential for effective patient management, clinical care, and epidemiologic surveillance. We describe an innovative diagnostic approach that combines generic RT-PCR amplification and identification by hybridization to specific probes. Original tetrathiolated probes were designed for the robust, sensitive, and specific detection of amplified arboviral genomes. The limit of detection using cultured and quantified stocks of whole viruses was 1 TCID50/mL for DENV-1, DENV-3, and CHIKV and 10 TCID50/mL for DENV-2, DENV-4, and ZIKV. The assay had 100% specificity with no false-positive results. The approach was evaluated using 179 human samples that previously tested as positive for the presence of ZIKV, DENV, or CHIKV genomes. Accordingly, the diagnostic sensitivity for ZIKV, DENV, and CHIKV was 87.88% (n = 58/66), 96.67% (n = 58/60), and 94.34% (n = 50/53), respectively. This method could be easily adapted to include additional molecular targets. Moreover, this approach may also be adapted to develop highly specific, sensitive, and easy to handle platforms dedicated to the multiplex screening and identification of emerging viruses

Additional file 1: Table S1. of Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis

Optimization of word count for MegaBLAST alignment to the NT reference database. (XLSX 12 kb

Additional file 2: Table S2. of Rapid metagenomic identification of viral pathogens in clinical samples by real-time nanopore sequencing analysis

MetaPORE performance according to system configuration and alignment mode. (XLSX 18 kb

CONCERTO: instrument and status

International audienceCONCERTO (CarbON CII line in post-rEionization and ReionizaTiOn) is a low-resolution Fourier transform spectrometer dedicated to the study of star-forming galaxies and clusters of galaxies in the transparent millimeter windows from the ground. It is characterized by a wide instantaneous 18.6 arcmin field of view, operates at 130-310 GHz, and was installed on the 12-meter Atacama Pathfinder Experiment (APEX) telescope at 5100 m above sea level. CONCERTO's double focal planes host two arrays of 2152 kinetic inductance detectors and represent a pioneering instrument to meet a state-of-the-art scientific challenge. This paper introduces the CONCERTO instrument and explains its status, shows the first CONCERTO spectral maps of Orion, and describes the perspectives of the project

CONCERTO: instrument and status

International audienceCONCERTO (CarbON CII line in post-rEionization and ReionizaTiOn) is a low-resolution Fourier transform spectrometer dedicated to the study of star-forming galaxies and clusters of galaxies in the transparent millimeter windows from the ground. It is characterized by a wide instantaneous 18.6 arcmin field of view, operates at 130-310 GHz, and was installed on the 12-meter Atacama Pathfinder Experiment (APEX) telescope at 5100 m above sea level. CONCERTO's double focal planes host two arrays of 2152 kinetic inductance detectors and represent a pioneering instrument to meet a state-of-the-art scientific challenge. This paper introduces the CONCERTO instrument and explains its status, shows the first CONCERTO spectral maps of Orion, and describes the perspectives of the project

CONCERTO: instrument and status

International audienceCONCERTO (CarbON CII line in post-rEionization and ReionizaTiOn) is a low-resolution Fourier transform spectrometer dedicated to the study of star-forming galaxies and clusters of galaxies in the transparent millimeter windows from the ground. It is characterized by a wide instantaneous 18.6 arcmin field of view, operates at 130-310 GHz, and was installed on the 12-meter Atacama Pathfinder Experiment (APEX) telescope at 5100 m above sea level. CONCERTO's double focal planes host two arrays of 2152 kinetic inductance detectors and represent a pioneering instrument to meet a state-of-the-art scientific challenge. This paper introduces the CONCERTO instrument and explains its status, shows the first CONCERTO spectral maps of Orion, and describes the perspectives of the project