2,560 research outputs found
Vibrational and electronic entropy of Ī²-cerium and Ī³-cerium measured by inelastic neutron scattering
Time-of-flight (TOF) inelastic neutron-scattering spectra were measured on Ī²-cerium (double hcp) and Ī³-cerium (fcc) near the phase-transition temperature. Phonon densities of states (DOS) and crystal-field levels were extracted from the TOF spectra. A softening of the phonon DOS occurs in the transition from Ī²- to Ī³-cerium, accounting for an increase in vibrational entropy of ĪSvibĪ³-Ī²=(0.09Ā±0.05)kB/atom. The entropy calculated from the crystal-field levels and a fit to calorimetry data from the literature were significantly larger in Ī²-cerium than in Ī³-cerium below room temperature, but the difference was found to be negligible at the experimental phase-transition temperature. A contribution to the specific heat from Kondo spin fluctuations was consistent with the quasielastic magnetic scattering, but the difference between phases was negligible. To be consistent with the latent heat of the Ī²-Ī³ transition, the increase in vibrational entropy at the phase transition may be accompanied by a decrease in electronic entropy not associated with the crystal-field splitting or spin fluctuations. At least three sources of entropy need to be considered for the Ī²-Ī³ transition in cerium
Optimization of Gene Prediction via More Accurate Phylogenetic Substitution Models
Determining the beginning and end positions of each exon in each protein coding gene within a genome can be difficult because the DNA patterns that signal a geneās presence have multiple weakly related alternate forms and the DNA fragments that comprise a gene are generally small in comparison to the size of the genome. In response to this challenge, automated gene predictors were created to generate putative gene structures. N SCAN identifies gene structures in a target DNA sequence and can use conservation patterns learned from alignments between a target and one or more informant DNA sequences. N SCAN uses a Bayesian network, generated from a phylogenetic tree, to probabilistically relate the target sequence to the aligned sequence(s). Phylogenetic substitution models are used to estimate substitution likelihood along the branches of the tree. Although N SCANās predictive accuracy is already a benchmark for de novo HMM based gene predictors, optimizing its use of substitution models will allow for improved conservation pattern estimates leading to even better accuracy. Selecting optimal substitution models requires avoiding overfitting as more detailed models require more free parameters; unfortunately, the number of parameters is limited by the number of known genes available for parameter estimation (training). In order to optimize substitution model selection, we tested eight models on the entire genome including General, Reversible, HKY, Jukes-Cantor, and Kimura. In addition to testing models on the entire genome, genome feature based model selection strategies were investigated by assessing the ability of each model to accurately reflex the unique conservation patterns present in each genome region. Context dependency was examined using zeroth, first, and second order models. All models were tested on the human and D. melanogaster genomes. Analysis of the data suggests that the nucleotide equilibrium frequency assumption (denoted as Ļi) is the strongest predictor of a modelās accuracy, followed by reversibility and transition/transversion inequality. Furthermore, second order models are shown to give an average of 0.6% improvement over first order models, which give an 18% improvement over zeroth order models. Finally, by limiting parameter usage by the number of training examples available for each feature, genome feature based model selection better estimates substitution likelihood leading to a significant improvement in N SCANās gene annotation accuracy
Pairagon+N-SCAN_EST: a model-based gene annotation pipeline
BACKGROUND: This paper describes Pairagon+N-SCAN_EST, a gene annotation pipeline that uses only native alignments. For each expressed sequence it chooses the best genomic alignment. Systems like ENSEMBL and ExoGean rely on trans alignments, in which expressed sequences are aligned to the genomic loci of putative homologs. Trans alignments contain a high proportion of mismatches, gaps, and/or apparently unspliceable introns, compared to alignments of cDNA sequences to their native loci. The Pairagon+N-SCAN_EST pipeline's first stage is Pairagon, a cDNA-to-genome alignment program based on a PairHMM probability model. This model relies on prior knowledge, such as the fact that introns must begin with GT, GC, or AT and end with AG or AC. It produces very precise alignments of high quality cDNA sequences. In the genomic regions between Pairagon's cDNA alignments, the pipeline combines EST alignments with de novo gene prediction by using N-SCAN_EST. N-SCAN_EST is based on a generalized HMM probability model augmented with a phylogenetic conservation model and EST alignments. It can predict complete transcripts by extending or merging EST alignments, but it can also predict genes in regions without EST alignments. Because they are based on probability models, both Pairagon and N-SCAN_EST can be trained automatically for new genomes and data sets. RESULTS: On the ENCODE regions of the human genome, Pairagon+N-SCAN_EST was as accurate as any other system tested in the EGASP assessment, including ENSEMBL and ExoGean. CONCLUSION: With sufficient mRNA/EST evidence, genome annotation without trans alignments can compete successfully with systems like ENSEMBL and ExoGean, which use trans alignments
Mapping functional transcription factor networks from gene expression data
A critical step in understanding how a genome functions is determining which transcription factors (TFs) regulate each gene. Accordingly, extensive effort has been devoted to mapping TF networks. In Saccharomyces cerevisiae, proteināDNA interactions have been identified for most TFs by ChIP-chip, and expression profiling has been done on strains deleted for most TFs. These studies revealed that there is little overlap between the genes whose promoters are bound by a TF and those whose expression changes when the TF is deleted, leaving us without a definitive TF network for any eukaryote and without an efficient method for mapping functional TF networks. This paper describes NetProphet, a novel algorithm that improves the efficiency of network mapping from gene expression data. NetProphet exploits a fundamental observation about the nature of TF networks: The response to disrupting or overexpressing a TF is strongest on its direct targets and dissipates rapidly as it propagates through the network. Using S. cerevisiae data, we show that NetProphet can predict thousands of direct, functional regulatory interactions, using only gene expression data. The targets that NetProphet predicts for a TF are at least as likely to have sites matching the TF's binding specificity as the targets implicated by ChIP. Unlike most ChIP targets, the NetProphet targets also show evidence of functional regulation. This suggests a surprising conclusion: The best way to begin mapping direct, functional TF-promoter interactions may not be by measuring binding. We also show that NetProphet yields new insights into the functions of several yeast TFs, including a well-studied TF, Cbf1, and a completely unstudied TF, Eds1
Gene prediction and verification in a compact genome with numerous small introns
The genomes of clusters of related eukaryotes are now being sequenced at an increasing rate, creating a need for accurate, low-cost annotation of exonāintron structures. In this paper, we demonstrate that reverse transcription-polymerase chain reaction (RTāPCR) and direct sequencing based on predicted gene structures satisfy this need, at least for single-celled eukaryotes. The TWINSCAN gene prediction algorithm was adapted for the fungal pathogen Cryptococcus neoformans by using a precise model of intron lengths in combination with ungapped alignments between the genome sequences of the two closely related Cryptococcus varieties. This approach resulted in ā¼60% of known genes being predicted exactly right at every coding base and splice site. When previously unannotated TWINSCAN predictions were tested by RTāPCR and direct sequencing, 75% of targets spanning two predicted introns were amplified and produced high-quality sequence. When targets spanning the complete predicted open reading frame were tested, 72% of them amplified and produced high-quality sequence. We conclude that sequencing a small number of expressed sequence tags (ESTs) to provide training data, running TWINSCAN on an entire genome, and then performing RTāPCR and direct sequencing on all of its predictions would be a cost-effective method for obtaining an experimentally verified genome annotation
Space object identification using phase-diverse speckle
Space-object identification from ground-based telescopes is challenging because of the degradation in resolution arising from atmospheric turbulence. Phase-diverse speckle is a novel post-detection correction method that can be used to overcome turbulence-induced aberrations for telescopes with or without adaptive optics. We present a simulation study of phase-diverse speckle satellite reconstructions for the Air Force Maui Optical station 1.6-meter telescope. For a given turbulence strength, satellite reconstruction fidelity is evaluated as a function of quality and quantity of data. The credibility of this study is enhanced by reconstructions from actual compensated data collected with the 1.5-meter telescope at the Starfire Optical Range. Consistent details observed across a time series of reconstructions from a portion of a satellite pass enhance the authenticity of these features. We conclude that phase-diverse speckle can restore fine-resolution features not apparent in the raw aberrated images of space objects
The SolventāSolid Interface of Acid Catalysts Studied by High Resolution MAS NMR
High-resolution magic angle spinning (HRMAS) NMR spectroscopy was used to study the eļ¬ect of mixed solvent systems on the acidity at the solidāliquid interface of solid acid catalysts. A method was developed that can exploit beneļ¬ts of both solution and solid-state NMR (SSNMR) by wetting porous solids with small volumes of liquids (Ī¼L/mg) to create an interfacial liquid that exhibits unique motional dynamics intermediate to an isotropic liquid and a rigid solid. Results from these experiments provide information about the inļ¬uence of the solvent mixtures on the acidic properties at a solidāliquid interface. Importantly, use of MAS led to spectra with full resolution between water in an acidic environment and that of bulk water. Using mixed solvent systems, the chemical shift of water was used to compare the relative acidity as a function of the hydration level of the DMSO-d6 solvent. Nonlinear increasing acidity was observed as the DMSO-d6 became more anhydrous. 1H HR-MAS NMR experiments on a variety of supported sulfonic acid functionalized materials, suggest that the acid strength and number of acid sites correlates to the degree of broadening of the peaks in the 1H NMR spectra. When the amount of liquid added to the solid is increased (corresponding to a thicker liquid layer), fully resolved water phases were observed. This suggests that the acidic proton was localized predominantly within a 2 nm distance from the solid. EXSY 1Hā1H 2D experiments of the thin layers were used to determine the rate of proton exchange for diļ¬erent catalytic materials. These results demonstrated the utility of using (SSNMR) on solidāliquid mixtures to selectively probe catalyst surfaces under realistic reaction conditions for condensed phase systems
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