Investigation Between the S377G3 GATA-4 Polymorphism and Migraine

Migraine is a common and painful neurological disorder, with genetic and environmental components. Several conditions have been shown to be comorbid with migraine, notably a cardiac malformation affecting the interatrial septum and leading to patent foramen ovale (PFO). Mutations in the development regulatory gene GATA-4, located on human chromosome 8p23.1-p22, have been found to be responsible for some cases of congenital heart defects including PFO. To determine whether the GATA-4 gene is involved in migraine, the present study performed an association analysis of a common GATA-4 variant that results in a change of amino acid (S377G), in a large case/control population (275 unrelated Caucasian migraineurs versus 275 control individuals). The results showed that there was no significant association for this polymorphism between migraine and controls (χ² = 0.84, P = 0.66). Thus it appears that the GATA-4 (S377G) mutation does not play a significant role in common migraine susceptibility

Suppression subtractive hybridization method for the identification of a new strain of murine hepatitis virus from xenografted SCID mice

During attempts to clone retroviral determinants associated with a mouse model of Langerhans cell histiocytosis (LCH), suppression subtractive hybridization (SSH) was used to identify unique viruses in the liver of severe combined immunodeficiency (SCID) mice transplanted with LCH tissues. A partial genomic sequence of a murine coronavirus was identified, and the whole genome (31428 bp) of the coronavirus was subsequently sequenced using PCR cloning techniques. Nucleotide sequence comparisons revealed that the genome sequence of the new virus was 91-93 % identical to those of known murine hepatitis viruses (MHVs). The predicted open reading frame from the nucleotide sequence encoded all known proteins of MHVs. Analysis at the protein level showed that the virus was closely related to the highly virulent MHV-JHM strain. The virus strain was named MHV-MI. No type D retroviruses were found. Degenerate PCR targeting of type D retrovirus and 5'-RACE targeting of other types of retroviruses confirmed the absence of any retroviral association with the LCH xenografted SCID mice. © 2015, The Author(s)

Establishing an effective, pedagogical method for Bb clarinet students to transfer to the bass clarinet

This study aims to establish an effective, pedagogical method for Bb clarinet students to successfully transfer to the bass clarinet. The differences between these two instruments are investigated by conducting a thorough review of the existing literature on the subject, combined with the opinion of professional bass clarinettists within Australia and overseas. By combining these two qualitative data sets, correlations and similarities in approach will be identified to suggest a method to transfer successfully

Establishing an effective, pedagogical method for Bb clarinet students to transfer to the bass clarinet

This study aims to establish an effective, pedagogical method for Bb clarinet students to successfully transfer to the bass clarinet. The differences between these two instruments are investigated by conducting a thorough review of the existing literature on the subject, combined with the opinion of professional bass clarinettists within Australia and overseas. By combining these two qualitative data sets, correlations and similarities in approach will be identified to suggest a method to transfer successfully

T.: The Concentration Profile of Nitric Acid and Other Species over Saskatchewan in August 1998: Retrieval from Data Recorded by Thermal-Emission Radiometry, Atmos

ABSTRACT We present vertical mixing ratio profiles for nitric acid (HNO