Guiding cell adhesion and motility by modulating cross-linking and topographic properties of microgel arrays

Biomaterial-driven modulation of cell adhesion and migration is a challenging aspect of tissue engineering. Here, we investigated the impact of surface-bound microgel arrays with variable geometry and adjustable cross-linking properties on cell adhesion and migration. We show that cell migration is inversely correlated with microgel array spacing, whereas directionality increases as array spacing increases. Focal adhesion dynamics is also modulated by microgel topography resulting in less dynamic focal adhesions on surface-bound microgels. Microgels also modulate the motility and adhesion of Sertoli cells used as a model for cell migration and adhesion. Both focal adhesion dynamics and speed are reduced on microgels. Interestingly, Gas2L1, a component of the cytoskeleton that mediates the interaction between microtubules and microfilaments, is dispensable for the regulation of cell adhesion and migration on microgels. Finally, increasing microgel cross-linking causes a clear reduction of focal adhesion turnover in Sertoli cells. These findings not only show that spacing and rigidity of surface-grafted microgels arrays can be effectively used to modulate cell adhesion and motility of diverse cellular systems, but they also form the basis for future developments in the fields of medicine and tissue engineering

Magnetically Actuable Complex-Shaped Microgels for Spatio-Temporal Flow Control

Complex-shaped microgels are promising building blocks for soft metamaterials. Their active and remote orientational control provides significant potential in architecting them in time and space. This work describes the use of magnetically actuable microgels of complex shape for spatio-temporal flow control and showcases the concept for microfluidic impellers. First, the fabrication of complex-shaped magnetically actuable poly(ethylene glycol) diacrylate based microgels via stop-flow lithography is presented. The microgels comprise a pre-programmed magnetic moment set by pre-aligned maghemite nanospindles during the fabrication step. This feature allows the microgels to be positioned in a static magnetic field and rotate under application of a rotating external field. The dependence of the magnetic field rotation rate and strength, maghemite content, and microgel shape on the magnetic response of the microgels is comprehensively quantified. Finally, the magnetic complex-shaped microgels are integrated as actuable impellers in a microfluidic chip. The microgels are positioned in space by polymerizing them around fixed poly(dimethylsiloxane) (PDMS) pillars. Free rotation around the PDMS pillar is achieved due to the oxygen inhibition layer at the chip and pillar surface. The versatility of the fabrication methodology is showcased by the investigation of in-chip mixing in a microfluidic device consisting of soft responsive impellers

Mindestanforderungskatalog Physik – ein Vorschlag

Die Studienanfänger in den technischen Studiengängen der Hochschulen für angewandte Wissenschaften haben nicht nur in Mathematik sondern auch in Physik sehr unterschiedliche Vorkenntnisse. Obwohl diese Fächer für das grundlegende Verständnis technischer Vorgänge von großer Bedeutung sind, kann die Ausbildung in diesen Bereichen angesichts der begrenzten dafür im Verlauf des Studiums zur Verfügung stehenden Zeitfenster nicht bei Null anfangen. Für Mathematik wurde daher von der Arbeitsgruppe cosh ein Mindestanforderungskatalog zusammengestellt und 2014 veröffentlicht. Er beschreibt Kenntnisse und Fertigkeiten, die Studienanfänger zur erfolgreichen Aufnahme eines WiMINT-Studiums (Wirtschaft, Mathematik, Informatik, Naturwissenschaft, Technik) an einer Hochschule benötigen. Inzwischen hat sich nun eine Arbeitsgruppe von Physikerinnen und Physikern an Hochschulen in Baden-Württemberg gebildet, deren Ziel es ist, einen analogen Mindestanforderungskatalog für den Bereich Physik zu erstellen. Hier wird der aktuell erreichte Stand der Arbeiten vorgestellt