Computational aerodynamic analysis of a Micro-CT based bio-realistic fruit fly wing

The aerodynamic features of a bio-realistic 3D fruit fly wing in steady state (snapshot) flight conditions were analyzed numerically. The wing geometry was created from high resolution micro-computed tomography (micro-CT) of the fruit fly Drosophila virilis. Computational fluid dynamics (CFD) analyses of the wing were conducted at ultra-low Reynolds numbers ranging from 71 to 200, and at angles of attack ranging from -10° to +30°. It was found that in the 3D bio-realistc model, the corrugations of the wing created localized circulation regions in the flow field, most notably at higher angles of attack near the wing tip. Analyses of a simplified flat wing geometry showed higher lift to drag performance values for any given angle of attack at these Reynolds numbers, though very similar performance is noted at -10°. Results have indicated that the simplified flat wing can successfully be used to approximate high-level properties such as aerodynamic coefficients and overall performance trends as well as large flow-field structures. However, local pressure peaks and near-wing flow features induced by the corrugations are unable to be replicated by the simple wing. We therefore recommend that accurate 3D bio-realistic geometries be used when modelling insect wings where such information is useful

Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry

tRNA-bound amino acids often need to be identified, for instance, in cases where different amino acids compete for binding to the same tRNA. Here, we present a mass-spectrometry-based protocol to determine the amino acids bound to tRNA by aminoacylation. We detail how to perform the aminoacylation reaction, the preparation of the aminoacyl-tRNA for measurement, and the mass spectrometric analysis. We use arginine acylation as an example; however, this protocol can be applied to any other amino acid

Electric Field Modulation of Galvanomagnetic Properties of Mesoscopic Graphite

Electric field effect devices based on mesoscopic graphite are fabricated for galvanomagnetic measurements. Strong modulation of magneto-resistance and Hall resistance as a function of gate voltage is observed as sample thickness approaches the screening length. Electric field dependent Landau level formation is detected from Shubnikov de Haas oscillations in magneto-resistance. The effective mass of electron and hole carriers has been measured from the temperature dependant behavior of these oscillations.Comment: 4 pages, 4 figures included, submitted to Phys. Rev. Let

The Enmity Relationship as Justified Negative Partiality

Existing discussions of partiality have primarily examined special personal relationships between family, friends, or co-nationals. The negative analogue of such relationships – for example, the relationship of enmity – has, by contrast, been largely neglected. This chapter explores this adverse relation in more detail and considers the special reasons generated by it. We suggest that enmity can involve justified negative partiality, allowing members to give less consideration to each other’s interests. We then consider whether the negative partiality of enmity can be justified through projects or the value inherent in the relationship, following two influential views about the justification of positive partiality. We argue that both accounts of partiality can be conceptually extended to the negative analogue, but doing so brings into focus the problems with such accounts of the grounds of partiality

Partiality, Asymmetries, and Morality’s Harmonious Propensity

We argue for asymmetries between positive and negative partiality. Specifically, we defend four claims: i) there are forms of negative partiality that do not have positive counterparts; ii) the directionality of personal relationships has distinct effects on positive and negative partiality; iii) the extent of the interactions within a relationship affects positive and negative partiality differently; and iv) positive and negative partiality have different scope restrictions. We argue that these asymmetries point to a more fundamental moral principle, which we call Morality’s Harmonious Propensity. According to this principle, morality has a propensity toward preserving positive relationships and dissolving negative ones

Diversity Exiting the Academy: Influential Factors for the Career Choice of Well-Represented and Underrepresented Minority Scientists

A national sample of PhD-trained scientists completed training, accepted subsequent employment in academic and nonacademic positions, and were queried about their previous graduate training and current employment. Respondents indicated factors contributing to their employment decision (e.g., working conditions, salary, job security). The data indicate the relative importance of deciding factors influencing career choice, controlling for gender, initial interest in faculty careers, and number of postgraduate publications. Among both well-represented (WR; n = 3444) and underrepresented minority (URM; n = 225) respondents, faculty career choice was positively associated with desire for autonomy and partner opportunity and negatively associated with desire for leadership opportunity. Differences between groups in reasons endorsed included: variety, prestige, salary, family influence, and faculty advisor influence. Furthermore, endorsement of faculty advisor or other mentor influence and family or peer influence were surprisingly rare across groups, suggesting that formal and informal support networks could provide a missed opportunity to provide support for trainees who want to stay in faculty career paths. Reasons requiring alteration of misperceptions (e.g., limited leadership opportunity for faculty) must be distinguished from reasons requiring removal of actual barriers. Further investigation into factors that affect PhDs’ career decisions can help elucidate why URM candidates are disproportionately exiting the academy

Ripple-locked coactivity of stimulus-specific neurons and human associative memory

Associative memory enables the encoding and retrieval of relations between different stimuli. To better understand its neural basis, we investigated whether associative memory involves temporally correlated spiking of medial temporal lobe (MTL) neurons that exhibit stimulus-specific tuning. Using single-neuron recordings from patients with epilepsy performing an associative object–location memory task, we identified the object-specific and place-specific neurons that represented the separate elements of each memory. When patients encoded and retrieved particular memories, the relevant object-specific and place-specific neurons activated together during hippocampal ripples. This ripple-locked coactivity of stimulus-specific neurons emerged over time as the patients’ associative learning progressed. Between encoding and retrieval, the ripple-locked timing of coactivity shifted, suggesting flexibility in the interaction between MTL neurons and hippocampal ripples according to behavioral demands. Our results are consistent with a cellular account of associative memory, in which hippocampal ripples coordinate the activity of specialized cellular populations to facilitate links between stimuli

A Comparison of Groundwater Storage Using GRACE Data, Groundwater Levels, and a Hydrological Model in Californias Central Valley

The Gravity Recovery and Climate Experiment (GRACE) measures changes in total water storage (TWS) remotely, and may provide additional insight to the use of well-based data in California's agriculturally productive Central Valley region. Under current California law, well owners are not required to report groundwater extraction rates, making estimation of total groundwater extraction difficult. As a result, other groundwater change detection techniques may prove useful. From October 2002 to September 2009, GRACE was used to map changes in TWS for the three hydrological regions (the Sacramento River Basin, the San Joaquin River Basin, and the Tulare Lake Basin) encompassing the Central Valley aquifer. Net groundwater storage changes were calculated from the changes in TWS for each of the three hydrological regions and by incorporating estimates for additional components of the hydrological budget including precipitation, evapotranspiration, soil moisture, snow pack, and surface water storage. The calculated changes in groundwater storage were then compared to simulated values from the California Department of Water Resource's Central Valley Groundwater- Surface Water Simulation Model (C2VSIM) and their Water Data Library (WDL) Geographic Information System (GIS) change in storage tool. The results from the three methods were compared. Downscaling GRACE data into the 21 smaller Central Valley sub-regions included in C2VSIM was also evaluated. This work has the potential to improve California's groundwater resource management and use of existing hydrological models for the Central Valley

Distinct Pools of β-Amyloid in Alzheimer Disease-Affected Brain: A Clinicopathologic Study

Objective: To determine whether β-amyloid (Aβ) peptides segregated into distinct biochemical compartments would differentially correlate with clinical severity of Alzheimer disease (AD). Design: Clinicopathologic correlation study. Participants: Twenty-seven patients from a longitudinal study of AD and 13 age- and sex-matched controls without a known history of cognitive impairment or dementia were included in this study. Interventions: Temporal and cingulate neocortex were processed using a 4-step extraction, yielding biochemical fractions that are hypothesized to be enriched with proteins from distinct anatomical compartments: TRIS (extracellular soluble), Triton (intracellular soluble), sodium dodecyl sulfate (SDS) (membrane associated), and formic acid (extracellular insoluble). Levels of Aβ₄₀ and Aβ₄₂ were quantified in each biochemical compartment by enzyme-linked immunosorbent assay. Results: The Aβ₄₂ level in all biochemical compartments was significantly elevated in patients with AD vs controls (P < .01). The Aβ₄₀ levels in the TRIS and formic acid fractions were elevated in patients with AD (temporal, P < .01; cingulate, P = .03); however, Triton and SDS Aβ₄₀ levels were similar in patients with AD and in controls. Functional impairment proximal to death correlated with Triton Aβ₄₂ (r = 0.48, P = .02) and SDS Aβ₄₂ (r = 0.41, P = .04) in the temporal cortex. Faster cognitive decline was associated with elevated temporal SDS Aβ₄₂ levels (P < .001), whereas slower decline was associated with elevated cingulate formic acid Aβ₄₂ and SDS Aβ₄₂ levels (P = .02 and P = .01, respectively). Conclusion: Intracellular and membrane-associated Aβ, especially Aβ₄₂ in the temporal neocortex, may be more closely related to AD symptoms than other measured Aβ species

An Investigation into the Character of Pre-Explosion Core-Collapse Supernova Shock Motion

We investigate the structure of the stalled supernova shock in both 2D and 3D and explore the differences in the effects of neutrino heating and the standing accretion shock instability (SASI). We find that early on the amplitude of the dipolar mode of the shock is factors of 2 to 3 smaller in 3D than in 2D. However, later in both 3D and 2D the monopole and dipole modes start to grow until explosion. Whereas in 2D the (l,m) = (1,0) mode changes sign quasi-periodically, producing the "up-and-down" motion always seen in modern 2D simulations, in 3D this almost never happens. Rather, in 3D when the dipolar mode starts to grow, it grows in magnitude and wanders stochastically in direction until settling before explosion to a particular patch of solid angle. In 2D we find that the amplitude of the dipolar shock deformation separates into two classes. For the first, identified with the SASI and for a wide range of "low" neutrino luminosities, this amplitude remains small and roughly constant. For the other, identified with higher luminosities and neutrino-driven convection, the dipolar amplitude grows sharply. Importantly, it is only for this higher luminosity class that we see neutrino-driven explosions within ~1 second of bounce. Moreover, for the "low" luminosity runs, the power spectra of these dipolar oscillations peak in the 30-50 Hz range associated with advection timescales, while for the high-luminosity runs the power spectra at lower frequencies are significantly more prominent. We associate this enhanced power at lower frequencies with slower convective effects and the secular growth of the dipolar shock amplitude. On the basis of our study, we hypothesize that neutrino-driven buoyant convection should almost always dominate the SASI when the supernova explosion is neutrino-driven.Comment: Accepted to the Astrophysical Journal; updated with additional figures and analysi