Lying or Belying: Dreams in The Tempest

This article investigates the relationship between dreams and lies in William Shakespeare\u27s The Tempest. Building on the contradictory definitions of the word dream as it was understood in Shakespeare\u27s time, the article highlights several instances in the play where Shakespeare draws attention to the artifice of his theatrical dreams, making the argument that these meta-theater tricks ultimately serve to celebrate audiences\u27 imaginative processes. Consequently, this argument hinges on the historical reality of Jacobean theatrical productions, such as Gwilym Jones\u27s description of the Blackfriars Theater\u27s special effects in Shakespeare\u27s Storms and Plato\u27s critique of false poets in Book X of The Republic. Throughout, the article asserts that—due to the combination of the play\u27s inherent artificiality and its celebratory nature—Shakespeare champions and applauds his own audience\u27s imagination

Crisis economics: perilous liquidity

Thesis (M.M. (Finance & Investment))--University of the Witwatersrand, Faculty of Commerce, Law and Management, Graduate School of Business Administration, 2013.No abstract provide

GROUND FORCE COMMANDER VIRTUAL REALITY TRAINER

As Navy Special Warfare pivots away from counter-terrorism operations of the past two decades toward peer competition in contested environments, they need Ground Force Commanders (GFC) to be better prepared to mitigate cognitive overload, employ integrated effects, and balance strategic mission risks. If GFC training can be baselined to a qualified doctrinal standard, then residual risk to mission and risk to force can be reduced through a systematized training pipeline, which can be augmented, enabled, and enhanced through the integration of currently available virtual reality technologies. The GFC position has traditionally been a role of critical thinking, decision-making and contingency management. As the battlefield develops, GFCs will have more assets to control and more contingencies to plan for than in the past. This study evaluates current GFC training and the virtual reality ecosystem. My recommendation is that a virtual reality trainer for Ground Force Commanders should be adopted in the Navy Special Warfare community because it will enable GFCs to conduct iterative training in a zero-threat environment.Lieutenant, United States NavyApproved for public release. Distribution is unlimited

Fabrication of Miniaturized Paper-Based Microfluidic Devices (MicroPADs)

Microfluidic paper-based analytical devices (microPADs) are emerging as cost-effective and portable platforms for point-of-care assays. A fundamental limitation of microPAD fabrication is the imprecise nature of most methods for patterning paper. The present work demonstrates that paper patterned via wax printing can be miniaturized by treating it with periodate to produce higher-resolution, high-fidelity microPADs. The optimal miniaturization parameters were determined by immersing microPADs in various concentrations of aqueous sodium periodate (NaIO4) for varying lengths of time. This treatment miniaturized microPADs by up to 80% in surface area, depending on the concentration of periodate and length of the reaction time. By immersing microPADs in 0.5-M NaIO4 for 48 hours, devices were miniaturized by 78% in surface area, and this treatment allowed for the fabrication of functional channels with widths as small as 301 µm and hydrophobic barriers with widths as small as 387 µm. The miniaturized devices were shown to be compatible with redox-based colorimetric assays and enzymatic reactions. This miniaturization technique provides a new option for fabricating sub-millimeter-sized features in paper-based fluidic devices without requiring specialized equipment and could enable new capabilities and applications for microPADs

The Grizzly, October 3, 2013

Strategic Plan Update • Ursinus President Fong Discusses Liberal Arts Education in China • Ursinus Students Visit New York City • Upsilon Phi Delta Pledging Plans • Astronomer Speaks • U-Innovate Winners Announced • Psych Professor Joins Campus • Ursinus Mass Email Policy Explained • Opinion: Don\u27t Get Sold on Multi-Level Marketing; Yes, Going to College is Definitely Worth it • UC Athletes Handle a Heavy Workload • Dalrymple Completes Impressive UC Career • Football and Field Hockey Keep Rollinghttps://digitalcommons.ursinus.edu/grizzlynews/1888/thumbnail.jp

DNA Extraction Method Development for Ocular Tissues

Purpose: DNA extraction kits are traditionally developed to work with liquid tissues such as blood, saliva, and swabs, but some have been proposed to work with solid tissues. Somatic variation in cancers can be important for tumor subtyping and treatment guidance, including ocular tumors. Additionally, epigenetic marks such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are tissue-specific and change in disease states, particularly evident in diabetic retinopathy and age-related macular degeneration. Commercial DNA extraction kits are available from several vendors, but the various kits have different strengths and weaknesses, and the removal of PCR inhibitors will vary with each kit. This project investigates the yield and purity of DNA from ocular tissues using commercial DNA extraction kits. Methods: Cornea, neural retina, RPE/choroid layer, optic nerve, and capsular bag were collected and aliquoted into 15 mg aliquots. Extractions were performed using the following kits: DNEasy Blood and Tissue Kit (Qiagen;), GeneJET Genomic DNA Purification Kit (ThermoFisher Scientific), Monarch HMW DNA Extraction Kit for Tissue (New England Biosciences), and genomicPrep Mini Spin Kit (Cytiva). DNA was quantified using the Qubit Fluorometer and molecular weight was checked by agarose gel. Several more kits are currently being tested. Results: All four kits yielded high molecular weight DNA (above 20 kbp). The Monarch HMW kit yielded DNA with significantly higher molecular weights. The DNA yields per milligram of tissue were highest using the DNEasy Blood and Tissue Kit for optic nerve, neural retina, and RPE/choroid. The yield was highest for the cornea using the genomicPrep Mini Spin Kit. Only the genomicPrep Mini Spin Kit yielded sufficient DNA for quantification from the capsular bag, and total yields were minimal (600 ng or less). Additional kits are currently being tested, but initial results indicate that several commercial kits will be sufficient for DNA extraction of ocular tissues. Further work is needed to purify epithelial cells and stem cells from the intraocular lens. Conclusions: Of the kits tested, all are sufficient to obtain significant amounts of DNA from all ocular tissues aside from the capsular bag. The Monarch HMW yielded the highest molecular weight, but significantly lower quantities of DNA than the other kits, indicating that it may not be ideal for most purposes. Protocol development for the capsular bag is still underway