BRS - Urubuquara: cultivar de feijão-caupi para a região Bragantina, PA.

Há uma grande demanda por novas cultivares de feijão-caupi na região Bragantina do Estado do Pará. Para atender essa demanda, foi realizada uma seleção na cultivar BR3 Tracuateua, que vem sendo cultivada na região há mais de 20 anos. Foi usado o método da seleção de planta individual com teste de progênie. Os critérios para seleção foram boa qualidade de grão, boa sanidade, e arquitetura e bom carrego. Em setembro de 2000, foram selecionadas 274 plantas individuais. Nos anos de 2000 e 2001, as progênies foram avaliadas, respectivamente em dois ensaios, ambos no delineamento de blocos aumentados. Das 274 progênies, foram selecionadas 24. Dessas, 16 foram avaliadas pelos produtores em unidades de observação e nove foram selecionadas. Com essas progênies, foi organizado o ensaio de Valor de Cultivo e Uso - VCU em blocos casualizados com quatro repetições. Foram realizados sete ensaios de VCU no período de 2002 a 2004. Destacaram-se as progênies Urubuquara-113 e Urubuquara-70, lançadas para região Bragantina do Estado do Pará, respectivamente, com os nomes de BRS Milênio e BRS Urubuquara. A cultivar BRS Milênio teve média de produtividade de 1.399,4 kg.ha-1 e a BRS Urubuquara de 1.276,8 kg.ha-1. Ambas são adequadas tanto à agricultura familiar quanto à empresarial e têm grãos que se enquadram na classe branco, subclasse brancão.bitstream/item/27813/1/com.tec.135.pdfDisponível também on-line

A pipeline to study structural interactions among Spodoptera frugiperda serine proteinases and plant proteinase inhibitors.

We propose here a computational biology pipeline to identify and analyze possible structural determinants that could explain some level of insensitivity by S. frugiperda serine proteinases (SPs) against plant PIs observed in a real time PCR experiment.GA 330

Toxocaríase humana: inquérito soroepidemiológico no município de Campinas (SP), Brasil

The occurrence of human Toxocara infection was evaluated in three neighborhoods of the periphery of the Campinas municipality (Jardim Santa Mônica, Jardim São Marcos and Jardim Campineiro) in 1999. Forty residences and 138 residents were randomly selected by drawing lots and were submitted to a seroepidemiological survey, which included blood collection for the immunoenzymatic detection (ELISA) of anti-Toxocara antibodies and a blood count, and the application of a semi-structured questionnaire for the evaluation of epidemiological data. Significant levels of anti-Toxocara antibodies were detected in 23.9% of the 1999 samples. No significant difference in the frequency of infection according to age was observed. Environmental contamination with Toxocara eggs was observed in 12.3 and 14.0% of 57 soil samples collected in the same region in December 1998 and July 1999, respectively. Univariate analysis and multiple logistic regression of the data obtained from the questionnaires and of the results of the serological tests, suggest a significant influence of socioeconomic variables on the frequency of human infection with Toxocara under the conditions prevalent in the study area.Estudou-se a ocorrência de infecção humana por Toxocara em três bairros da periferia do município de Campinas (Jardim Santa Mônica, Jardim São Marcos e Jardim Campineiro). Em 1999 sortearam-se aleatoriamente 40 domicílios e 138 moradores foram submetidos a inquérito soroepidemiológico, que incluiu coleta de sangue para teste imunoenzimático (E.L.I.S.A.) visando pesquisa de anticorpos anti-Toxocara e hemograma, além de aplicação de questionário semi-estruturado para avaliação de dados de interesse epidemiológico. Observou-se presença de anticorpos anti-Toxocara em 23,9% das amostras, sem ocorrência de diferença significativa quando se considerou a idade ou sexo dos indivíduos examinados. Em 57 amostras de solo da mesma região, coletadas em dezembro de 1998 e novamente em julho de 1999, estudou-se a contaminação ambiental por ovos de Toxocara, verificando-se sua presença em, respectivamente, 12,3% e 14,0% das amostras. Análises univariada e multivariada por regressão logística dos dados obtidos a partir dos questionários e dos resultados da sorologia anti-Toxocara mostraram influência significativa de variáveis de ordem sócio-econômica na prevalência de infecção humana por Toxocara, nas condições presentes na área estudada

Dietary energy intake and sexual behavior of Toggenburg goats.

In order to evaluate the sexual behavior of Toggenburg goats fed with different dietary energy, 32 kids in reproductive age and free of any reproductive pathology were divided in 3 groups according to the dietary treatment. Group 1, maintenance (M) (n=11); Group 2, supplied energy 1.5 times energy maintenance (1.5M) (n=10); and Group 3, supplied energy 2.0 times energy maintenance (2.0M) (n=11). The estrus cycles of all kids were induced and synchronized by insertion of an intravaginal sponge impregnated with 60mg Medroxyprogesterone Acetate. An ultrasound scanner fitted with an 8.0-MHz linear transducer, adapted for small ruminants was used to determine ovulation time and females were tested for estrus twice daily after sponge removal by the use of a male goat. Time between sponge removal and beginning of estrus and from beginning of estrus until ovulation, as well as estrus length were measured. Results were analyzed by the Duncan test employing a significancy level of P 0.05). Time between sponge removal and beginning of estrus also did not differ (Group 1: 28.00 ± 12.00 hours; Group 2: 32.80 ± 11.59 hours; Group 3: 30.40 ± 14.08 hours ? P>0.05) but time from beginning of estrus until ovulation was substantially shorter in group 3 (15.13 ± 8.63 hours ? P <0.05) than other groups (Group 1: 28.28 ± 12.57 hours; Group 2: 27.00 ± 14.40 hours). We conclude that animals fed with high energy diet ovulates faster than others after the onset of estrus

Comportamento sexual de ovelhas superovuladas submetidas a dois métodos de acasalamento.

[Sexual behavior of superovulated sheep subjected to two methods of breeding]

Effects of deliberate reflection on diagnostic accuracy, confidence and diagnostic calibration in dermatology

Background: Deliberate reflection on initial diagnoses improved diagnostic accuracy in internal medicine and general practice, but it is unknown if the same occurs in specialties that rely mostly on visual perception, such as dermatology. Moreover, whether reflection influences diagnostic calibration has not been studied yet. Diagnostic calibration, the relationship between diagnostic accuracy and confidence in that accuracy, affects diagnostic performance because overconfidence tends to induce premature closure. This study evaluated the effects of deliberate reflection on diagnostic accuracy and diagnostic calibration in dermatology. Methods: Sixty-one sixth-year students from a Brazilian medical school were allocated to either a reflection group (RG) or a control group (CG). In both groups, students worked with the same 12 dermatological images, presented sequentially, providing an initial diagnosis and confidence in that diagnosis. Subsequently, RG students reflected on the case using a structured procedure, while CG students performed a time-filler activity. All students then provided a final diagnosis and confidence in that diagnosis. Outcome measurements were diagnostic accuracy, confidence, and calibration. Results: Reflection increased diagnostic accuracy relative to control (49.7 ± 12.1 vs 38.4 ± 14.6; p = 0.002) but did not affect confidence (64.3 ± 13.2 vs 58.9 ± 20.1; p = 0.228) nor calibration (0.15 ± 0.16 vs 0.20 ± 0.19, p = 0.197). Overall, case difficulty influenced calibration, with students showing more overconfidence on more difficult cases (p <0.001). Conclusions: Deliberate ref

The new nitric oxide donor cyclohexane nitrate induces vasorelaxation, hypotension, and antihypertensive effects via NO/cGMP/PKG pathway

We investigated the cardiovascular effects induced by the nitric oxide donor Cyclohexane Nitrate (HEX). Vasodilatation, NO release and the effects of acute or sub-chronic treatment with HEX on cardiovascular parameters were evaluated. HEX induced endothelium-independent vasodilatation (Maximum effect- [efficacy, ME] = 100.4±4.1%; potency [pD2] = 5.1±0.1). Relaxation was attenuated by scavenging nitric oxide (ME = 44.9±9.4% vs. 100.4±4.1%) or by inhibiting the soluble guanylyl cyclase (ME = 38.5±9.7% vs. 100.4±4.1%). In addition, pD2 was decreased after non-selective blockade of K+ channels (pD2 = 3.6±0.1 vs. 5.1±0.1) or by inhibiting KATP channels (pD2 = 4.3±0.1 vs. 5.1±0.1). HEX increased NO levels in mesenteric arteries (33.2±2.3 au vs. 10.7±0.2 au, p<0.0001). Intravenous acute administration of HEX (1-20 mg/kg) induced hypotension and bradycardia in normotensive and hypertensive rats. Furthermore, starting at six weeks after the induction of 2K1C hypertension, oral treatment with the HEX (10 mg/Kg/day) for seven days reduced blood pressure in hypertensive animals (134±6 vs 170±4 mmHg, respectively). Our data demonstrate that HEX is a NO donor able to produce vasodilatation via NO/cGMP/PKG pathway and activation of the ATP-sensitive K+ channels. Furthermore, HEX acutely reduces blood pressure and heart rate as well as produces antihypertensive effect in renovascular hypertensive rats