A prospective clinical cohort study analyzing single-unit implant crowns after three years of loading: introduction of a novel Functional Implant Prosthodontic Score (FIPS)

Objectives The aim of this prospective clinical cohort study was to validate implant crowns with a novel Functional Implant Prosthodontic Score (FIPS). Material and methods Twenty patients were restored with cement-retained crowns on soft tissue level implants (Institut Straumann AG, Basel, Switzerland) in posterior sites and annually followed-up for 3 years. FIPS was applied for the objective outcome assessment including clinical and radiographic examinations. Five variables were defined for evaluation, resulting in a maximum score of 10 per implant restoration. The patients' level of satisfaction was recorded and correlated with FIPS. Results All implants and connected crowns revealed survival rates of 100% without any biological or technical complications after three years of loading. The mean total FIPS score was 7.8 ± 1.5, ranging from 6 to 10. The variable “bone” revealed the highest scores (2 ± 0; range: 2–2), followed by “occlusion” (1.9 ± 0.1; range: 1–2). Mean scores for “design” (1.2 ± 0.6; range: 0–2), “mucosa” (1.3 ± 0.7; range: 0–2), and “interproximal” (1.4 ± 0.4; range: 1–2) were more challenging to satisfy. The patients expressed a high level of functional satisfaction (84.1 ± 9.5; range: 68–100). A significant correlation was found between FIPS and the subjective patients' perception with a coefficient of 0.88 (P < 0.0001). Conclusions The findings of the clinical trial indicated the potential of FIPS as an objective and reliable instrument in assessing implant success. FIPS can be considered as a supportive tool to validate a satisfactory outcome as perceived by patients, to identify possible failure risks, and to compare follow-up observations

Anaerobiosis revisited: growth of Saccharomyces cerevisiae under extremely low oxygen availability

The budding yeast Saccharomyces cerevisiae plays an important role in biotechnological applications, ranging from fuel ethanol to recombinant protein production. It is also a model organism for studies on cell physiology and genetic regulation. Its ability to grow under anaerobic conditions is of interest in many industrial applications. Unlike industrial bioreactors with their low surface area relative to volume, ensuring a complete anaerobic atmosphere during microbial cultivations in the laboratory is rather difficult. Tiny amounts of O2 that enter the system can vastly influence product yields and microbial physiology. A common procedure in the laboratory is to sparge the culture vessel with ultrapure N2 gas; together with the use of butyl rubber stoppers and norprene tubing, O2 diffusion into the system can be strongly minimized. With insights from some studies conducted in our laboratory, we explore the question ‘how anaerobic is anaerobiosis?’. We briefly discuss the role of O2 in non-respiratory pathways in S. cerevisiae and provide a systematic survey of the attempts made thus far to cultivate yeast under anaerobic conditions. We conclude that very few data exist on the physiology of S. cerevisiae under anaerobiosis in the absence of the anaerobic growth factors ergosterol and unsaturated fatty acids. Anaerobicity should be treated as a relative condition since complete anaerobiosis is hardly achievable in the laboratory. Ideally, researchers should provide all the details of their anaerobic set-up, to ensure reproducibility of results among different laboratories. A correction to this article is available online at http://eprints.whiterose.ac.uk/131930/ https://doi.org/10.1007/s00253-018-9036-

Microbiota associated with residual clefts and neighboring teeth in patients with cleft lip, alveolus, and palate

Twenty patients with residual clefts or pronounced soft tissue grooves, treated for uni- or bilateral cleft lip, alveolus, and palate were included in this study. Ten patients were recalled for dental prophylaxis at regular intervals, 10 patients were not. One microbiologic sample was obtained from the cleft area and two samples from a tooth adjacent to the cleft (sites adjacent and distant to the cleft). Between the recall and the nonrecall group there were notable differences in the presence of anaerobic Gram- negative organisms. Fusobacterium spp., Prevotella melaninogenica, and P. intermedia were more often found in nonrecall patients. While rarely seen in recall patients, spirochetes and motile rods were a common feature of nonrecall patients. The putative periodontal pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were not detected in either group. The differences between the recall and the nonrecall groups were more pronounced when the respective samples from teeth were related to each other than when the samples obtained from the clefts were compared. The cleft flora was less complex irrespective of how good maintenance was and resembled the flora of teeth of well-maintained patients. Samples from clefts were never Wolinella positive, and harbored significantly less Capnocytophaga and Actinomyces viscosus than samples from dental sites.link_to_subscribed_fulltex

Patterns of periodontal destruction associated with incomplete root fractures

Objectives: To clarify the differential radiographic diagnostics of teeth with incomplete root fracture. Methods: Information on 88 teeth with either a vertical root fracture without displacement of the fractured fragments (n = 22), a vertical periodontal defect (n = 22), a periodontal-endodontic lesion (n = 22) or an endodontic-periapical lesion (n = 22) was collected retrospectively from the patients' records. Results: Significant differences in the mean probing pocket depths were found between each of the four groups of lesions. Teeth with root fractures demonstrated smaller mean probing pocket depths than those with either periodontal or periodontal-endodontic lesions. Radiographic bone loss was significantly greater in teeth with periodontal and periodontal-endodontic lesions when compared with those with fractures. In teeth with vertical root fractures, there was no correlation between the probing depth and the radiographic bone loss (r = 0.01). The correlation coefficient between the greatest probing depth and alveolar bone loss was r= -0.16 in the fractured teeth, with higher values for the other three groups of lesions. 86.4% of the teeth with fractures had a complete root canal filling. Posts were present in 22.7% of the fractured teeth and in 13% of those with an endodontic periapical lesion. 91 % of the teeth with vertical fractures were crowned or fixed-bridge abutments. Only 5% of the fractured teeth had no evidence of periapical bone changes. Conclusions: Incomplete root fractures mainly involve teeth which have undergone successful endodontic treatment. An isolated pocket on one aspect of a suspected tooth is reliably diagnostic for a root fracture. © 1997 Stockton Press All rights reserved.link_to_subscribed_fulltex

Patterns of periodontal destruction associated with incomplete root fractures

Objectives: To clarify the differential radiographic diagnostics of teeth with incomplete root fracture. Methods: Information on 88 teeth with either a vertical root fracture without displacement of the fractured fragments (n = 22), a vertical periodontal defect (n = 22), a periodontal-endodontic lesion (n = 22) or an endodontic-periapical lesion (n = 22) was collected retrospectively from the patients&apos; records. Results: Significant differences in the mean probing pocket depths were found between each of the four groups of lesions. Teeth with root fractures demonstrated smaller mean probing pocket depths than those with either periodontal or periodontal-endodontic lesions. Radiographic bone loss was significantly greater in teeth with periodontal and periodontal-endodontic lesions when compared with those with fractures. In teeth with vertical root fractures, there was no correlation between the probing depth and the radiographic bone loss (r = 0.01). The correlation coefficient between the greatest probing depth and alveolar bone loss was r= -0.16 in the fractured teeth, with higher values for the other three groups of lesions. 86.4% of the teeth with fractures had a complete root canal filling. Posts were present in 22.7% of the fractured teeth and in 13% of those with an endodontic periapical lesion. 91 % of the teeth with vertical fractures were crowned or fixed-bridge abutments. Only 5% of the fractured teeth had no evidence of periapical bone changes. Conclusions: Incomplete root fractures mainly involve teeth which have undergone successful endodontic treatment. An isolated pocket on one aspect of a suspected tooth is reliably diagnostic for a root fracture. © 1997 Stockton Press All rights reserved

Guided bone regeneration around an immediate transmucosal implant for single tooth replacement: a case report.

This case report describes the procedure of guided bone regeneration around an endosseous dental implant, placed into an extraction alveolus in the absence of total soft tissue coverage, ie, in a transmucosal condition. The technique presented permits an immediate placement of the implant after the extraction of the maxillary right lateral incisor. A nonresorbable barrier membrane was used, and strict chemical plaque control was maintained during osseointegration. After an uneventful healing period of 6 months, the membrane was removed in a second-stage surgery. Two months later, the fixed single tooth prosthesis was placed. The learning objective of this article is to explain the immediate transmucosal implant surgery technique, using a case report to illustrate the procedure.link_to_subscribed_fulltex

Bone regeneration adjacent to titanium dental implants using guided tissue regeneration: a report of two cases.

The biologic principle of guided tissue regeneration was applied to regenerate alveolar bone in conjunction with the placement of titanium dental implants. In one case, complete osseointegration of an implant was achieved by the placement of a Teflon membrane over an implant that had been inserted into an alveolus immediately following tooth extraction. In a second case, the same biologic principle was used to increase the volume (height and width) of a resorbed, edentulous alveolar ridge to provide adequate bone dimensions for implant installation. In both cases, the membranes appear to have prevented the repopulation of the wound area by cells other than those derived from surrounding bone tissue. These two different applications of the principle of guided tissue regeneration open new avenues for reconstructive osseous surgery.link_to_subscribed_fulltex