Nanogenomics and Nanoproteomics Enabling Personalized, Predictive and Preventive Medicine

Since the discovery of the nucleic acid, molecular biology has made tremendous progresses, achieving a lot of results. Despite this, there is still a gap between the classical and traditional medical approach and the molecular world. Inspired by the incredible wealth of data generated by the "omics"-driven techniques and the “high-trouhgput technologies” (HTTs), I have tried to develop a protocol that could reduce the actually extant barrier between the phenomenological medicine and the molecular medicine, facilitating a translational shift from the lab to the patient bedside. I also felt the urgent need to integrate the most important omics sciences, that is to say genomics and proteomics. Nucleic Acid Programmable Protein Arrays (NAPPA) can do this, by utilizing a complex mammalian cell free expression system to produce proteins in situ. In alternative to fluorescent-labeled approaches a new label free method, emerging from the combined utilization of three independent and complementary nanobiotechnological approaches, appears capable to analyze gene and protein function, gene-protein, gene-drug, protein-protein and protein-drug interactions in studies promising for personalized medicine. Quartz Micro Circuit nanogravimetry (QCM), based on frequency and dissipation factor, mass spectrometry (MS) and anodic porous alumina (APA) overcomes indeed the limits of correlated fluorescence detection plagued by the background still present after extensive washes. Work is in progress to further optimize this approach a homogeneous and well defined bacterial cell free expression system able to realize the ambitious objective to quantify the regulatory gene and protein networks in humans. Implications for personalized medicine of the above label free protein array using different test genes and proteins are reported in this PhD thesis

Economic microbiology: exploring microbes as agents in economic systems

Microbial communities exhibit striking parallels with economic markets, resembling intricate ecosystems where microorganisms engage in resource exchange akin to human market transactions. This dynamic network of resource swapping mirrors economic trade in human markets, with microbes specializing in metabolic functions much like businesses specializing in goods and services. Cooperation and competition are central dynamics in microbial communities, with alliances forming for mutual benefit and species vying for dominance, similar to businesses seeking market share. The human microbiome, comprising trillions of microorganisms within and on our bodies, is not only a marker of socioeconomic status but also a critical factor contributing to persistent health inequalities. Social and economic factors shape the composition of the gut microbiota, impacting healthcare access and quality of life. Moreover, these microbes exert indirect influence over human decisions by affecting neurotransmitter production, influencing mood, behavior, and choices related to diet and emotions. Human activities significantly impact microbial communities, from dietary choices and antibiotic use to environmental changes, disrupting these ecosystems. Beyond their natural roles, humans harness microbial communities for various applications, manipulating their interactions and resource exchanges to achieve specific goals in fields like medicine, agriculture, and environmental science. In conclusion, the concept of microbial communities as biological markets offers valuable insights into their intricate functioning and adaptability. It underscores the profound interplay between microbial ecosystems and human health and behavior, with far-reaching implications for multiple disciplines. To paraphrase Alfred Marshall, “the Mecca of the economist lies in economic microbiology.

I giochi dell'analisi transazionale. Come riconoscerli e liberarsene. [The games of transactional analysis. How to acknowledge them and how to get rid of them.]

Sabrina D&rsquo;Amanti is a trained transactional analyst, who initially worked in the field of clinical and school psychology. This book covers the history and the theoretical foundation of Transactional Analysis (TA), focusing above all on the theory of mind or psychological games, and offers practical examples that can greatly help practitioners and analysts - especially health psychologists - in their clinical work...</p

L'essenza del coaching. [The essence of coaching].

Both Alessandro Pannitti and Franco Rossi have a solid and reputed experience of several years in the field of Coaching, and in this book they have provided the readers with their expert, authoritative overview on the different coaching techniques...</p

Protein Crystallization by Anodic Porous Alumina (APA) Template: The Example of Hen Egg White Lysozyme (HEWL)

In this communication, we report anodic porous alumina (APA) template induced crystallization. The APA nanotemplate was prepared on the glass substrate for the hen egg white lysozyme (HEWL) crystal growth. The changes in the lysozyme crystals morphology, namely in the a/c axis ratio, were observed in the crystal grown by APA nanotemplate, but not in the crystal obtained with classical hanging drop vapor diffusion method, under the same experimental conditions. The comparison of the diffraction data of the two crystals as well as bioinformatics and data mining approaches and molecular dynamics simulations suggest a possible explanation of the nanotemplate crystallization phenomenon and shed light on the APA-induced nanocrystallography

The old and the new: vaccine hesitancy in the era of the Web 2.0. Challenges and opportunities

The phenomenon known as Vaccine hesitancy (a term that includes the concepts of indecision, uncertainty, delay, reluctance) is complex and closely linked to the different contexts, with different determinants: historical period, geographical areas, political situation, as complacency, convenience and confidence towards vaccines. The World Health Organization (WHO) recommends to constantly monitor vaccine hesitancy and any proxy of it. Given the growing importance and pervasiveness of the information and communication technologies (ICTs), the new media could be exploited for a real-time tracking of vaccination-related perception by the lay-people, enabling health-care workers to actively engage themselves and to plan ad hoc communication strategies. The analysis of so-called "sentiments" expressed through the new media (such as Twitter), the real-time tracking of web-related activities enabled by Google Trends, combined with online specific "surveys" on well-defined themes administered to target groups (like health-care workers) may constitute the "Fast data monitoring system", enabling to get a snapshot on the perception of vaccination in that place and at that time. This type of dashboard could be a strategic tool for public services, to organize targeted communication actions aimed at containing Vaccine hesitancy

Social determinants, ethical issues and future challenge of tuberculosis in a pluralistic society: the example of Israel

Tuberculosis is a very serious respiratory infectious disease, caused by the bacillus Mycobacterium tuberculosis, which generates a relevant societal and clinical burden. It has always represented a permanent concern and a public health challenge over the course of human history, because of its severe epidemiological, and economic-financial implications. The present review aims at over-viewing the impact on tuberculosis on the Israeli healthcare system, its temporal trend and evolution, stratified according to ethnicities and minorities, the need of establishing new facilities and implementing screening techniques, public health strategies and diagnostic tests, following massive immigration waves from countries characterized by a high incidence rate of tuberculosis during the fifties-sixties until the nineties, and the policies implemented by the Israeli government in the control, management and treatment of tuberculosis, as well as the role played by Israeli prominent scientists in discovering new druggable targets and finding bioactive compounds and bio-molecules in the fight against tuberculosis. Israel represents a unique, living laboratory in which features of developed and developing countries mix together. This country as a case-study of immigrant, pluralistic society underlines the importance of adopting a culturally-sensitive community intervention approach. The understanding of the subtle interplay between race/ethnic host and pathogen factors, including the role of gene variations and polymorphisms can pave the way for a personalized treatment and management of tuberculosis patients, contributing to the development of new tools for targeted tuberculosis therapeutics, immunodiagnostics and vaccination products

Microarray-based Functional Nanoproteomics for an Industrial Approach to Cancer. II Mass Spectrometry and Nanoconductimetry

Using the New England BioLabs (NEBL) SNAP-based Genes Expression in conjunction with our “sub-micron arrays” (Anodic Porous Allumina and/ or Kapton based Nanopores), we exploit our proprietary microarrays scanner (DNASER, DNA analyzer) and Label Free Nanotechnologies to carry out the following tasks:\ud \ud 1) Construction of SNAP-based Genes Nanoarrays, using gold surface coated for 10 minutes with 2% solution of 3-Aminopropyltriethoxysilane (APTES) in acetone, rinsed in acetone and dried with filtered air. Full length complementary DNAs (cDNAs) for onco-suppressor 53 (p53), Cyclin-dependent kinase 2 (CDK2), SH2 (Src Homology 2) domain of the proto-oncogene tyrosine-protein kinase (Src) and tyrosine-protein phosphatase non-receptor type 11 (PTPN11) were amplified and cloned. Printing mix was prepared with 0.66 μg/μl DNA capture reagent BG-PEG-NH2 for the one-step synthesis of SNAP-tag substrates from esters on labels or surfaces;\ud \ud 2) Determination of Protein-Protein Interaction for the chosen cancer following the identification of leader genes (or hub genes, investigated with theoretical ab initio bioinformatics analysis using in-house software and algorithms, and then experimentally confirmed via DNASER). These genes are expressed by PURE (Protein synthesis Using Recombinant Elements) Express in spots less than 1 micron size piezo-microdispensed and then characterized via Label Free proprietary Autoflex Mass Spectrometry (MS) integrated with ad hoc software, namely the Spectrum Analyzer and Data Set manager (SpADS) and a proprietary Quartz Crystal Micro-balance with Dissipation factor monitoring (QCM_D) Nanoconductimetry, enabling to describe properties such as changes in frequency and conductance, viscoelasticity and dissipation factor. Solutions without DNA were prepared (called Master Mix, MM), as negative controls, in printing mix. Negative controls were prepared with a varying concentration range of SNAP capture reagent. As a positive control (for fluorescence analysis) mouse IgG or rabbit IgG (Pierce, IL, USA) were added in a printing mix instead of DNA