1,153 research outputs found

    In Vitro Expression of Full-Length and Truncated Bovine Respiratory Syncytial Virus G Proteins and Their Antibody Responses in BALB/c Mice

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    Bovine respiratory syncytial virus (BRSV) is a primary cause of lower respiratory tract disease in calves. Protection is incomplete following vaccination or natural infection, as re-infections are common. The objectives of this study were to create plasmid DNA constructs encoding the full-length, secreted, or conserved region of the BRSV G glycoprotein, and to compare and evaluate their expression in cell culture and potential to induce antibody responses in BALB/c mice. Transfection of COS-7 cells with plasmid DNA resulted in expression of the BRSV G region from each of the plasmid DNA constructs. Following inoculation of BALB/c mice with plasmid DNA, a significant and equivalent anti-BRSV G IgG response was elicited to the full-length and truncated BRSV G proteins. These constructs may be used to study host pathological and immunological responses

    ExoMol line lists -- LVI: The SO line list, MARVEL analysis of experimental transition data and refinement of the spectroscopic model

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    A semi-empirical IR/Vis line list, SOLIS, for the sulphur monoxide molecule 32^{32}S16^{16}O is presented. SOLIS includes accurate empirical rovibrational energy levels, uncertainties, lifetimes, quantum number assignments, and transition probabilities in the form of Einstein AA coefficients covering the X 3Σ−X\,{}^{3}\Sigma^{-}, a 1Δa\,{}^{1}\Delta^{ }, b 1Σ+b\,{}^{1}\Sigma^{+}, A 3ΠA\,{}^{3}\Pi, B 3Σ−B\,{}^{3}\Sigma^{-}, X ′′3Σ+X\,{}^{\prime\prime3}\Sigma^{+}, A ′3ΔA\,{}^{\prime 3}\Delta and e 1Πe\,{}^{1}\Pi systems and wavenumber range up to 43303.5 cm−1^{-1} (≥230.93\geq 230.93 nm) with J≤69J\le 69. SOLIS has been computed by solving the rovibronic Schr\"{o}dinger equation for diatomics using the general purpose variational code Duo and starting from a published ab initio spectroscopic model of SO (including potential energy curves, coupling curves, (transition) dipole moment curves) which is refined to experimental data. To this end, a database of 50106 experimental transitions, 48972 being non-redundant, has been compiled through the analysis of 29 experimental sources, and a self-consistent network of 8558 rovibronic energy levels for the XX, aa, bb, AA, BB, and CC electronic states has been generated with the MARVEL algorithm covering rotational and vibrational quantum numbers J≤69J \leq 69 and v≤30v \leq 30 and energies up to 52350.40 cm−1^{-1}. No observed transitions connect to the B 3Σ−(v=0) B\,{}^{3}\Sigma^{-} (v = 0) state which is required to model perturbations correctly, so we leave fitting the B 3Σ−B\,{}^3\Sigma^- and C 3ΠC\,{}^3\Pi state UV model to a future project. The SO line list is available at ExoMol from www.exomol.com.Comment: 16 pages, 12 figure

    ExoMol line lists – LIII: empirical rovibronic spectra of yttrium oxide

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    Empirical line lists BRYTS for the open shell molecule 89Y16O (yttrium oxide) and its isotopologues are presented. The line lists cover the six lowest electronic states: X 2+, A 2, A 2 ∆, B 2+, C 2, and D 2+ up to 60 000 cm−1 (<0.167 μm) for rotational excitation up to J = 400.5. An ab initio spectroscopic model consisting of potential energy curves (PECs), spin–orbit, and electronic angular momentum couplings is refined by fitting to experimentally determined energies of YO, derived from published YO experimental transition frequency data. The model is complemented by empirical spin-rotation and Λ-doubling curves and ab initio dipole moment and transition dipole moment curves computed using multireference configuration interaction (MRCI). The ab initio PECs computed using the complete basis set limit extrapolation and the coupled-cluster CCSD(T) method with its higher quality provide an excellent initial approximation for the refinement. Non-adiabatic coupling curves for two pairs of states of the same symmetry A/C and B/D are computed using a state-averaged complete active space self-consistant field theory (CASSCF) and used to build diabatic representations for the A 2, C 2, B 2+, and D 2+ curves. The experimentally derived energies of 89Y16O utilized in the fit are used to replace the corresponding calculated energy values in the BRYTS line list. Simulated spectra of YO show excellent agreement with the experiment, where it is available. Calculated lifetimes of YO are tuned to agree well with the experiment, where available. The BRYTS YO line lists are available from the ExoMol database (www.exomol.com)

    ExoMol line lists -- LIII: Empirical Rovibronic spectra of Yttrium Oxide (YO)

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    Empirical line lists for the open shell molecule 89^{89}Y16^{16}O (yttrium oxide) and its isotopologues are presented. The line lists cover the 6 lowest electronic states: X2Σ+X {}^{2}\Sigma^{+}, A2ΠA {}^{2}\Pi, A′2ΔA' {}^{2}\Delta, B2Σ+B {}^{2}\Sigma^{+}, C2ΠC {}^{2}\Pi and D2Σ+D {}^{2}\Sigma^{+} up to 60000 cm−1^{-1} (<0.167<0.167 μ\mum) for rotational excitation up to J=400.5J = 400.5. An \textit{ab initio} spectroscopic model consisting of potential energy curves (PECs), spin-orbit and electronic angular momentum couplings is refined by fitting to experimentally determined energies of YO, derived from published YO experimental transition frequency data. The model is complemented by empirical spin-rotation and Λ\Lambda-doubling curves and \textit{ab initio} dipole moment and transition dipole moment curves computed using MRCI. The \textit{ab initio} PECs computed using the complete basis set limit extrapolation and the CCSD(T) method with its higher quality provide an excellent initial approximation for the refinement. Non-adiabatic coupling curves for two pairs of states of the same symmetry AA/CC and BB/DD are computed using a state-averaged CASSCF and used to built diabatic representations for the A2ΠA {}^{2}\Pi, C2ΠC {}^{2}\Pi, B2Σ+B {}^{2}\Sigma^{+} and D2Σ+D {}^{2}\Sigma^{+} curves. Calculated lifetimes of YO are tuned to agree well with the experiment, where available. The BRYTS YO line lists for are included into the ExoMol data base (www.exomol.com)

    MUTATIONS AND ANTIBIOTIC RESISTANCE IN MYCOBACTERIUM TUBERCULOSIS

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    Mycobacterium tuberculosis (MTB) is a bacterium known to target, infect, and destroy lung cells as well as connective tissues within the body. This bacterium is prevalent worldwide and has infected over a quarter of the current world population, becoming one of the most successful pathogens in history. Due to its extreme transmission rates as an airborne pathogen, MTB strains have been treated with antibiotics such as rifampicin and isoniazid, which inhibit bacterial infection in the human body. These first-round drugs remained as successful mechanisms for slowing and killing the pathogen, notably through rifampicin\u27s inhibition of RNA-polymerase and isoniazid’s ability to halt the formation of the bacterial cell wall. However, TB has proven a threat due to the recent discovery of multi-drug-resistant tuberculosis strains, rendering these first-round drugs ineffective. The major objectives of the present study were to 1) review the most recent published literature on TB, 2) examine the role of mutations on antibiotic resistance in TB strains and 3) share our synthesis on the successes and challenges of TB treatment worldwide. Our research was guided through data available in the NCBI GenBank, and a review of literature. To accomplish these objectives, we reviewed relevant literature on Mycobacterium tuberculosis to collect pathophysiological data, trends in TB mutations, and present-day applications of how this disease is continually prevalent worldwide. We collected antibiotic responsive rpoB gene sequences from the National Library of Medicine’s GenBank to assess mutations specific strains of TB from four countries. We found that random mutations caused the evolution of TB strains with effective antibiotic resistance and the selective nature of the medications encourages these antibiotic-resistant genes. New medications, like bedaquiline, take considerable research but effectively find new druggable targets against these resistant mycobacteria. However, MDR TB still remains a considerable threat despite some newly developed drugs

    Numerical Approaches to Spacetime Singularities

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    This Living Review updates a previous version which its itself an update of a review article. Numerical exploration of the properties of singularities could, in principle, yield detailed understanding of their nature in physically realistic cases. Examples of numerical investigations into the formation of naked singularities, critical behavior in collapse, passage through the Cauchy horizon, chaos of the Mixmaster singularity, and singularities in spatially inhomogeneous cosmologies are discussed.Comment: 51 pages, 6 figures may be found in online version: Living Rev. Relativity 2002-1 at www.livingreviews.or
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