Archaeology of settlement during Roman period - Sunken objects on settlement during Roman period in Bohemia and their interpretation

Práce shrnuje informace o zahloubených objektech na sídlištích doby římské v Čechách. Zároveň je sledováno zastoupení jednotlivých objektů i nadzemních staveb na sídlištích. Hlavní pozornost je věnována polozemnicím a jejich interpretaci.Katedra archeologieObhájenoThe work summarizes information about the sunken features at the settlements of Roman period in Bohemia. Alongside is observed a frequency of each object and aboveground structures at the settlements. The main attention is paid to sunken-floored huts and their interpretation

Gothic country churches between Plasy and Žihle

Práce je zaměřena na gotické venkovské kostely na bývalém panství cisterciáků v Plasích. Stavebně-historické analýze bylo podrobeno pět stojících kostelů: kostel sv. Jana Křtitele v Ostrovci, kostel sv. Jana Blažeje ve Vysoké Libyni, kostel Povýšení sv. Kříže v Tisu u Blatna, kostel sv. Martina ve Strážišti a kostel sv. Jakuba Většího v Žebnici. Všechny stavby mají v současnosti barokní podobu. Práce vyústila ke komparaci formálních znaků jednotlivých kostelů a jejich chronologické zařazení.ObhájenoWork is focused on rural Gothic churches in the former estate of the Cistercians in Plasy. Architectural and historical analysis has undergone five standing churches: St. Jan Křtitel in Ostrovec, St. Jan Blažej in Vysoká Libyně, Povýšení St. Kříže in Tis u Blatna, St. Martin in Strážiště and St. Jakub Větší in Žebnice. All buildings are having Baroque form currently. Work has resulted in the comparison of formal characteristics of individual churches and their chronological classification

Branchioma: immunohistochemical and molecular genetic study of 23 cases highlighting frequent loss of retinoblastoma 1 immunoexpression

Branchioma is an uncommon benign neoplasm with an adult male predominance, typically occurring in the lower neck region. Different names have been used for this entity in the past (ectopic hamartomatous thymoma, branchial anlage mixed tumor, thymic anlage tumor, biphenotypic branchioma), but currently, the term branchioma has been widely accepted. Branchioma is composed of endodermal and mesodermal lineage derivatives, in particular epithelial islands, spindle cells, and mature adipose tissue without preexistent thymic tissue or evidence of thymic differentiation. Twenty-three branchiomas were evaluated morphologically. Eighteen cases with sufficient tissue were assessed by immunohistochemistry, next-generation sequencing (NGS) using the Illumina Oncology TS500 panel, and fluorescence in situ hybridization (FISH) using an RB1 dual-color probe. All cases showed a biphasic morphology of epithelial and spindle cells with intermingled fatty tissue. Carcinoma arising in branchioma was detected in three cases. The neoplastic cells showed strong AE1/3 immunolabeling (100%), while the spindle cells expressed CD34, p63, and SMA (100%); AR was detected in 40-100% of nuclei (mean, 47%) in 14 cases. Rb1 showed nuclear loss in ≥ 95% of neoplastic cells in 16 cases (89%), while two cases revealed retained expression in 10-20% of tumor cell nuclei. NGS revealed a variable spectrum of likely pathogenic variants (n = 5) or variants of unknown clinical significance (n = 6). Loss of Rb1 was detected by FISH in two cases. Recent developments support branchioma as a true neoplasm, most likely derived from the rudimental embryological structures of endoderm and mesoderm. Frequent Rb1 loss by immunohistochemistry and heterozygous deletion by FISH is a real pitfall and potential confusion with other Rb1-deficient head and neck neoplasms (i.e., spindle cell lipoma), especially in small biopsy specimens