Embryonic POU5F1 is Required for Expanded Bovine Blastocyst Formation.

POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage. POU5F1 knockout morulas developed at a similar rate as control embryos and presented a similar number of blastomeres by day 5 of development. Initiation of SOX2 expression by day 5 of development was not affected by lack of POU5F1. On the other hand, CDX2 expression was aberrant in embryos lacking POU5F1. Notably, the phenotype observed in bovine POU5F1 knockout embryos reveals conserved functions associated with loss of human embryonic POU5F1 that differ from Pou5f1- null mice. The similarity observed in transcriptional regulation of early embryo development between cattle and humans combined with highly efficient gene editing techniques make the bovine a valuable model for human embryo biology with expanded applications in agriculture and assisted reproductive technologies

Interview of Michael R. Smith

Michael R. Smith is an alumnus of La Salle University, Class of 1982, with a degree in accounting. He was born in 1960 at Nazareth Hospital in the Greater Northeast section of Philadelphia, Pennsylvania. He grew up along the Roosevelt Boulevard of Northeast Philadelphia in the Holmes Circle neighborhood. He attended and graduated from Father Judge Catholic High School in Philadelphia in 1978. He decided upon La Salle University and attended La Salle as an accounting major from 1978 to 1982, the latter being his graduation year. Legacy wise, Michael is one of many La Salle graduates in his family, including his father, sister, brother, two uncles, and two cousins. His sister also taught at La Salle University, as well, and she encouraged Michael to attend here especially. Michael commuted to the La Salle campus and was part of the commuter culture at the school, especially with other students from Northeast Philadelphia. Michael participated in intramural swimming and water polo on campus, and he played a lot of pinochle with his campus friends. Academically, his favorite professor was Pete Sweeney in accounting, who taught Michael principled conservative accounting, as well as professional ethics. Michael also did a quasi-internship while at La Salle at Kay Automotive in Philadelphia, where he worked as an accountant. This was his first full-time career job upon graduation. Michael has since gone on to careers in financial and business management, and he applauds the strong La Salle accounting network for alumni throughout the country. Today, he is the father of a 19-year-old daughter at Bloomsburg University, and he lives vicariously through her, encouraging her to take advantage of aspects of college life that he neglected while an undergraduate. Michael currently resides in Morrisville, Pennsylvania

Police visibility, trust in police fairness, and collective efficacy: A multilevel Structural Equation Model

Areas high in collective efficacy—where residents know and trust one another, and are willing to intervene to solve neighbourhood problems—tend to experience less crime. Policing is thought to be one antecedent to collective efficacy, but little empirical research has explored this question. Using three waves of survey data collected from London residents over three consecutive years, and multilevel Structural Equation Modelling, this study tested the impact of police visibility and police-community engagement on collective efficacy. We explored direct effects as well as indirect effects through trust in police. The findings showed levels of police visibility predicted trust in police. Trust in police fairness, in turn, predicted collective efficacy. There was a small indirect relationship between police visibility and collective efficacy, through trust in police fairness. In other words, police presence in neighbourhoods was associated with more positive views about officer behaviour, which in turn was associated with collective efficacy. The findings have important implications for policies designed to build stronger, more resilient communities

Acute alcohol administration dampens central extended amygdala reactivity.

Alcohol use is common, imposes a staggering burden on public health, and often resists treatment. The central extended amygdala (EAc)-including the bed nucleus of the stria terminalis (BST) and the central nucleus of the amygdala (Ce)-plays a key role in prominent neuroscientific models of alcohol drinking, but the relevance of these regions to acute alcohol consumption in humans remains poorly understood. Using a single-blind, randomized-groups design, multiband fMRI data were acquired from 49 social drinkers while they performed a well-established emotional faces paradigm after consuming either alcohol or placebo. Relative to placebo, alcohol significantly dampened reactivity to emotional faces in the BST. To rigorously assess potential regional differences in activation, data were extracted from unbiased, anatomically predefined regions of interest. Analyses revealed similar levels of dampening in the BST and Ce. In short, alcohol transiently reduces reactivity to emotional faces and it does so similarly across the two major divisions of the human EAc. These observations reinforce the translational relevance of addiction models derived from preclinical work in rodents and provide new insights into the neural systems most relevant to the consumption of alcohol and to the initial development of alcohol abuse in humans

Impact of evaporative pads and cross ventilation on core body temperature and resting time of lactating cows

A trial was conducted to determine the impact of evaporative cooling pads on core body temperature (CBT), time spent lying, and number of lying bouts of Holstein cows housed in cross-ventilated freestall facilities. Despite cool ambient conditions during the trial, cows without evaporative pads tended to have elevated CBT above 102°F for 2.3 more hours per day and elevated CBT above 102.5°F for 0.95 more hours per day than cows with evaporative pads. These trends were evident even though the stocking density of the freestalls was greater in the facility with evaporative pads than in the facility without pads (123 vs. 113%). Lying times and lying bouts did not differ between treatments. Results of this study indicate that CBT tended to be reduced when evaporative pads were used, even under relatively mild ambient conditions.; Dairy Day, 2009, Kansas State University, Manhattan, KS, 2009; Dairy Research, 2009 is known as Dairy Day, 200

Opportunities with low profile cross ventilated freestall facilities

Low profile cross ventilated freestall buildings are one option for dairy cattle housing. These facilities allow producers to control the cows\u27 environment during all seasons of the year. As a result, an environment similar to the thermoneutral zone of a dairy cow is maintained during both summer and winter, resulting in more stable core body temperatures. Low profile cross ventilated facilities allow buildings to be placed closer to the parlor, thus reducing the time cows are away from feed and water. Other advantages include a smaller overall site footprint than naturally ventilated facilities and less critical orientation because naturally ventilated facilities should be orientated east to west to keep cows in the shade. Other benefits of controlling the cows\u27 environment include increased milk production and income over feed cost, improved feed efficiency and reproductive performance, reduced lameness and fly control costs, and the ability to control lighting.; Dairy Day, 2008, Kansas State University, Manhattan, KS, 2008; Dairy Research, 2008 is known as Dairy Day, 200

Estimating optimal operation time of korral kools on dairy cows in a desert environment

Developing management strategies for Korral Kools will help producers provide cooling in the housing area while minimizing the operational cost of the Korral Kools system. Two experiments were conducted at a dairy in Saudi Arabia to evaluate operational time of Korral Kools for multiparous and primiparous dairy cows. For multiparous cows, running time per day of Korral Kools should be continuous, but for primiparous cows, no difference in performance was detected between 21 and 24 hours. However, producers need to be careful when reducing daily operation time of Korral Kools for primiparous cows because elevated core body temperatures were observed in both treatments.; Dairy Day, 2008, Kansas State University, Manhattan, KS, 2008; Dairy Research, 2008 is known as Dairy Day, 200

NICMOS Imaging of the HR 4796A Circumstellar Disk

We report the first near infrared (NIR) imaging of a circumstellar annular disk around the young (~8 Myr), Vega-like star, HR 4796A. NICMOS coronagraph observations at 1.1 and 1.6 microns reveal a ring-like symmetrical structure peaking in reflected intensity 1.05 arcsec +/- 0.02 arcsec (~ 70 AU) from the central A0V star. The ring geometry, with an inclination of 73.1 deg +/- 1.2 deg and a major axis PA of 26.8 deg +/- 0.6 deg, is in good agreement with recent 12.5 and 20.8 micron observations of a truncated disk (Koerner, et al. 1998). The ring is resolved with a characteristic width of less than 0.26 arcsec (17 AU) and appears abruptly truncated at both the inner and outer edges. The region of the disk-plane inward of ~60 AU appears to be relatively free of scattering material. The integrated flux density of the part of the disk that is visible (greater than 0.65 arcsec from the star) is found to be 7.5 +/- 0.5 mJy and 7.4 +/- 1.2 mJy at 1.1 and 1.6 microns, respectively. Correcting for the unseen area of the ring yields total flux densities of 12.8 +/- 1.0 mJy and 12.5 +/- 2.0 mJy, respectively (Vega magnitudes = 12.92 /+- 0.08 and 12.35 +/-0.18). The NIR luminosity ratio is evaluated from these results and ground-based photometry of the star. At these wavelengths Ldisk(lambda)/L*(lambda) = 1.4 +/- 0.2E-3 and 2.4 +/- 0.5E-3, giving reasonable agreement between the stellar flux scattered in the NIR and that which is absorbed in the visible and re-radiated in the thermal infrared. The somewhat red reflectance of the disk at these wavelengths implies mean particle sizes in excess of several microns, larger than typical interstellar grains. The confinement of material to a relatively narrow annular zone implies dynamical constraints on the disk particles by one or more as yet unseen bodies.Comment: 14 pages, 1 figure for associated gif file see: http://nicmosis.as.arizona.edu:8000/AAS99/FIGURE1_HR4796A_ApJL.gif . Accepted 13 January 1999, Astrophyical Journal Letter