The effects of femtosecond laser-textured Ti-6Al-4V on wettability and cell response

International audienceTo study the biological activity effects of femtosecond laser-induced structures on cell behavior, TA6V sampleswere micro-texturedwith focused femtosecond laser pulses generating grooves of various dimensions on the micrometerscale (width: 25–75 μm; depth: 1–10 μm). LIPSS (Laser Induced Periodic Surface Structures) were alsogenerated during the laser irradiation, providing a supplementary structure (sinusoidal form) of hundreds ofnanometers at the bottom of the grooves oriented perpendicular (⊥ LIPPS) or parallel (// LIPPS) to the directionof these grooves. C3H10 T1/2 murine mesenchymal stem cells were cultivated on the textured biomaterials. Tohave a preliminary idea of the spreading of biological media on the substrate, prior to cell culture, contactangle measurement were performed. This showed that the post-irradiation hydrophilicity of the samples can decreasewith time according to its storage environment. The multiscale structuration either induced a collaborativeor a competitive influence of the LIPSS and grooves on the cells. It has been shown that cells individuallyand collectively were most sensitive to microscale grooves which were narrower than 25 μm and deeper than5 μm with ⊥ LIPPS. In some cases, cells were individually sensitive to the LIPSS but the cell layer organizationdid not exhibit significant differences in comparison to a non-textured surface. These results showed that cellsare more sensitive to the nanoscale structures (LIPSS), unless the microstructures's size is close to the cell sizeand deeper than 5 μm. There, the cells are sensitive to the microscale structures and go on spreading followingthese structures

Fibronectin substitute derived from plants and functionalisation of biomaterials

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