Etude de l'orientation fonctionnelle des lymphocytes B dans le contexte physiologique et dans le syndrome de Gougerot-Sjögren

International audienc

Etude de l'orientation fonctionnelle des lymphocytes B dans le contexte physiologique et dans le syndrome de Gougerot-Sjögren

International audienc

Study of functional orientation of B cells in physiology and autoimmunity

International audienc

Study of functional orientation of B cells in physiology and autoimmunity

International audienc

Caractérisation des lymphocytes B autoréactifs en physiologie et au cours des maladies auto-immunes

International audienc

Innate B Cells: the Archetype of Protective Immune Cells

International audienceThe innate B cell (IBC) population is heterogeneous and involved in the primary immune response. IBC functions include a high ability to produce natural antibodies with IgM isotype, the elimination of apoptotic cells, and a capacity to be cognate help to T cells. Among IBC subsets, B-1 cells and marginal zone B cells are the main producers of IgM, act as rapid immune responders that may relocate to follicular lymphoid and differentiate to cytokine and antibody-secreting cells shortly after infection. IBCs functions are highly dependent on their localization site and the nature of their B cell receptor repertoire, suggesting a high plasticity range of different immune responses. In this review, we will describe the nature and functions of the different innate-like B cell subsets, first in mice and then in humans. Besides this, we will emphasize the strong ability of these cells to undertake different protective functions from the first line of defense against pathogens to the regulatory role of the broader immune response

Study of functional orientation of B cells in physiology and Sjögren’s syndrome.

International audienc

Study of functional orientation of B cells in physiology and Sjögren’s syndrome.

International audienc

The diversity of the plasmablast signature across species and experimental conditions: A meta-analysis

International audienceAntibody-secreting cells (ASC) are divided into two principal subsets, including the long-lived plasma cell (PC) subset residing in the bone marrow and the short-lived subset, also called plasmablast (PB). PB are described as a proliferating subset circulating through the blood and ending its differentiation in tissues. Due to their inherent heterogeneity, the molecular signature of PB is not fully established. The purpose of this study was to decipher a specific PB signature in humans and mice through a comprehensive meta-analysis of different data sets exploring the PB differentiation in both species and across different experimental conditions. The present study used recent analyses using whole RNA sequencing in prdm1-GFP transgenic mice to define a reliable and accurate PB signature. Next, we performed similar analysis using current data sets obtained from human PB and PC. The PB-specific signature is composed of 155 and 113 genes in mouse and human being, respectively. Although only nine genes are shared between the human and mice PB signature, the loss of B-cell identity such as the down-regulation of PAX5, MS4A1, (CD20) CD22 and IL-4R is a conserved feature across species and across the different experimental conditions. Additionally, we observed that the IRF8 and IRF4 transcription factors have a specific dynamic range of expression in human PB. We thus demonstrated that IRF4/IRF8 intranuclear staining was useful to define PB in vivo and in vitro and able to discriminate between atypical PB populations and transient states

A cytokine network profile delineates a common Th1/Be1 pro‐inflammatory group of patients in four systemic autoimmune diseases

International audienc