An in silico assessment of alternatively spliced isoforms in the mouse brain using RNA-Seq

With the advent of next-generation sequencing techniques like RNA-Seq, there is the potential for unbiased transcriptome-wide analysis of gene expression and alternative splicing irrespective of the abundance class of the transcript. A potential use for this technology is to help us understand the role that alternative splicing plays in brain region-specific differences. In this study, we focused on two RNA-Seq datasets derived from the mouse brain, one from the striatum and the other from the whole brain

(The) forces behind the Kansas-Nebraska bill

Thesis (M.A.)--Boston University, 1916. This item was digitized by the Internet Archive

The Fair Value Test in Montana Public Utility Rate Regulation

The Fair Value Test in Montana Public Utility Rate Regulatio

Ten Years of the Collaborative Cross

The February 2012 issues of GENETICS and G3: Genes, Genomes, Genetics present a collection of articles reporting recent advances from the international Collaborative Cross (CC) project. The goal of the CC project is to develop a new resource that will enhance quantitative trait locus (QTL) and systems genetic analyses in mice. The CC consists of hundreds of independently bred, octo-parental recombinant inbred lines (Figure 1). The work reported in these issues represents progress toward completion of the CC, proof-of-principle experiments using incipient inbred CC mice, and new research areas and complementary resources facilitated by the CC project

oligoMask: A Framework for Assessing and Removing the Effect of Genetic Variants on Microarray Probes

As expression microarrays are typically designed relative to a reference genome, any individual genetic variant that overlaps a probe’s genomic position can possibly cause a reduction in hybridization due to the probe no longer being a perfect match to a given sample’s mRNA at that locus. If the samples or groups used in a microarray study differ in terms of genetic variants, the results of the microarray experiment can be negatively impacted. The oligoMask package is an R/SQLite framework which can utilize publicly available genetic variants and works in conjunction with the oligo package to read in the expression data and remove microarray probes which are likely to impact a given microarray experiment prior to analysis. Tools are provided for creating an SQLite database containing the probe and variant annotations and for performing the commonly used RMA preprocessing procedure for Affymetrix microarrays. The oligoMask package is freely available at https://github.com/dbottomly/oligoMas

The role of the principal as an instructional leader : a case study of three schools in the Motupa Circuit, Limpopo

The study focused on the role of the principal as an instructional leader in the Motupa Circuit primary schools of Limpopo Province. The nature and effectiveness of the education system depend on the role of school principals as instructional leaders in the effective and efficient monitoring and support of educators' curriculum implementation in the classrooms, evidenced by learners' performance and the effective functioning of educational institutions. The literature review undertaken benchmarked on the instructional leadership roles of school principals. Qualitative research approach in the form of observations, interviews and the analysis of written documents was employed to elicit the principals' perceptions on their instructional leadership roles and how they enhance effective teaching and learning towards improving learners' performances. The study anticipated that principals as instructional leaders will be increasingly involved in ensuring that curriculum implementation in the classrooms is monitored and supported for effective teaching and learning to be realised. The study's findings revealed that this is not happening as it should. A synopsis of the research findings revealed that principals are beset with a number of contextual factors such as, an inadequate provision of teaching and learning resources and infrastructure needs, lack of monitoring and support of curriculum implementation, inadequate support from the Department of Basic Education, and lack of parental involvement and concern on the education of their children. It is anticipated that the literature review and the findings from the empirical study will contribute to, and strengthen principals' instructional leadership roles in creating conditions for effective teaching and learning, by monitoring and supporting educators in curriculum delivery and implementation in the classrooms. The research recommended that principals should conduct regular class visits and also ensure that strategies are developed to involve parents of learners in the education of their children, and that teaching and learning resources should be provided. The Provincial Department of Basic Education must provide the much needed basic school infrastructure and basic school resources such as LTSM, the national norms and standard for school funding (NNSSF) earlier during the first term, in support of principals' instructional leadership roles. It is recommended that attention to these issues by school principals and the Department of Basic Education will contribute towards effective teaching and learning and improve learners' performances in Limpopo Province and the Motupa Circuit in particular.M. Ed. (Education Management)Educational Leadership and Managemen