Remodelling of Membrane Rafts Expression in Lung Cells as an Early Sign of Mechanotransduction-Signalling in Pulmonary Edema

Membrane rafts (MRs) are clusters of lipids, organized in a “quasicrystalline” liquid-order phase, organized on the cell surface and whose pattern of molecules and physicochemical properties are distinct from those of the surrounding plasma membrane. MRs may be considered an efficient and fairly rapid cell-activated mechanism to express or mask surface receptors aimed at triggering specific response pathways. This paper reports observations concerning the role of MRs in the control of lung extravascular water that ought to be kept at minimum to assure gas diffusion, supporting the hypothesis that MRs expression is a potential mechanism of sensing minor changes in the volume of extravascular water. We present the evidence that MRs expression specifically relates to signal-transduction processes evoked by mechanical stimuli arising in the interstitial lung compartment when a small increase in extravascular volume occurs. We further hypothesize that a differential expression of MRs might also reflect the damage to precise components of the extracellular matrix caused by the perturbation in water balance and thus can trigger a molecule-oriented specific matrix remodelling

Modeling of Flexible Bodies for the Study of Control in the Simulink Environment

When studying complex mechatronic systems, it is useful to build models able to simulate both the dynamics of the phenomenon and the control system applied. Typically, the bodies involved are modeled as rigid bodies. In this work, a technique for modeling flexible bodies in Simulink environment is presented. Simulink is a powerful instrument where it is quite easy to integrate control algorithms with complex systems. The solution developed is presented and applied to a machining center. Modern machining centers ensure a level of accuracy that traditional manual machines cannot reach. Simulations of the working process considering vibrations are needed to obtain high precision machining. These simulations aim to determine the error in the position of the tool and to help designers in finding the optimal solution in terms of machining velocity and precision. This work is focused on the carriage of a machine tool moving along horizontal guides, typically named Z-axis. The axis is actuated and borne by a linear motor; therefore, movable constraints must be modeled. A finite-element (FE) model of the carriage was reduced with a Craig-Bampton reduction to provide the mass and stiffness matrices for an in-house Matlab simulation code. The rigid constraints of the carriage were implemented in the model as moving stiffnesses, and their value was set to obtain continuity of the constraints in the discrete model. In the end, a map of different vibrational configurations is proposed to visualize the possible errors that a machining process can generate

Biochemical and morphological changes in endothelial cells in response to hypoxic interstitial edema

BACKGROUND: A correlation between interstial pulmonary matrix disorganization and lung cellular response was recently documented in cardiogenic interstitial edema as changes in the signal-cellular transduction platforms (lipid microdomains: caveoale and lipid rafts). These findings led to hypothesize a specific "sensing" function by lung cells resulting from a perturbation in cell-matrix interaction. We reason that the cell-matrix interaction may differ between the cardiogenic and the hypoxic type of lung edema due to the observed difference in the sequential degradation of matrix proteoglycans (PGs) family. In cardiogenic edema a major fragmentation of high molecular weight PGs of the interfibrillar matrix was found, while in hypoxia the fragmentation process mostly involved the PGs of the basement membrane controlling microvascular permeability. Based on these considerations, we aim to describe potential differences in the lung cellular response to the two types of edema. METHODS: We analysed the composition of plasma membrane and of lipid microdomains in lung tissue samples from anesthetized rabbits exposed to mild hypoxia (12 % O(2 )for 3–5 h) causing interstitial lung edema. Lipid analysis was performed by chromatographic techniques, while protein analysis by electrophoresis and Western blotting. Lipid peroxidation was assessed on total plasma membranes by a colorimetric assay (Bioxytech LPO-586, OxisResearch). Plasma membrane fluidity was also assessed by fluorescence. Lipid microdomains were isolated by discontinuous sucrose gradient. We also performed a morphometric analysis on lung cell shape on TEM images from lung tissue specimen. RESULTS: After hypoxia, phospholipids content in plasma membranes remained unchanged while the cholesterol/phospholipids ratio increased significantly by about 9% causing a decrease in membrane fluidity. No significant increase in lipid peroxidation was detected. Analysis of lipid microdomains showed a decrease of caveolin-1 and AQP1 (markers of caveolae), and an increase in CD55 (marker of lipid rafts). Morphometry showed a significant decrease in endothelial cell volume, a marked increase in the cell surface/volume ratio and a decrease in caveolar density; epithelial cells did not show morphological changes. CONCLUSION: The biochemical, signaling and morphological changes observed in lung endothelial cell exposed to hypoxia are opposite to those previously described in cardiogenic edema, suggesting a differential cellular response to either type of edema

Iron-Modification of Pyroclastic Material from PCCVC Eruption (Chile): Characterization and Application to Remove Arsenic from Groundwater

Pyroclastic material from the PCCVC eruption (Chile) was modified with iron (III) solutions leading to the formation of ferrihydrite surface deposits. The aim of the chemical treatment was to prepare an adsorbent to remove arsenic from water by using low-cost mineral wastes. Physicochemical characterization of original and modified materials was carried out by XRD, BET-N2 adsorption, SEM-EDS microscopy and ICP-AES chemical analysis. The modified ash revealed that the increase of bulk iron content was close to 5% (expressed as Fe2O3) whereas surface values were 20.6% Fe2O3. Surface properties showed an increase of BET specific surface with prevalence of mesopores and an increase of total pore volume attributed to presence of nanoscopic iron phase. Kinetic and equilibrium studies were directed to optimize the operative conditions related to the material adsorptive capacity for removing arsenate species. Hence, the adsorbent dose, contact time, pH, stirring and sedimentation were evaluated in batch process. The optimal adsorption dose was 40 g・L−1 and the solid-liquid contact time was stirring (1 h) and sedimentation (23 h), enough to ensure an adequate turbidity value valid for a pH range between 3.77 and 8.95. The analysis of the isotherm equilibrium by using the Langmuir linear method showed a R2 = 0.995 value. The performance of the treatment to remove arsenic by using a cost-effective adsorbent prepared from volcanic material is a promising technology to apply in the environmental field.Centro de Química InorgánicaInstituto de Recursos MineralesCentro de Investigación y Desarrollo en Ciencias Aplicada

Análisis de intervenciones sanitarias y modalidades de cuidado en salud mental en la niñez: estudio multicéntrico en municipios de Argentina

El proceso de transformación paradigmática que atraviesa el campo de la salud mental involucra cambios y readecuaciones en las instituciones de salud y en las respuestas sanitarias locales. Un proceso creciente y de envergadura actual indica que niños, niñas y jóvenes están siendo afectados por problemas vinculados a su salud mental, al tiempo que son depositarios de acciones sanitarias que ponen el foco en la patología. Para entender esta problemática se expone resultados de una investigación que propuso caracterizar y evaluar las modalidades de cuidado en salud mental en materia de niñez de los distintos niveles de atención en jurisdicciones seleccionadas del país: Santa Fé, La Rioja y Ciudad Autónoma de Buenos Aires. Se relevó información a través de la Clasificación Internacional de Cuidados en Salud Mental (ICMHC/OMS) y de entrevistas semiestructuradas a profesionales responsables de los servicios de salud mental. Se contemplaron diferentes niveles de atención del sistema público de salud. En todas las jurisdicciones estudiadas se ha constatado un proceso de reforma incipiente y con diversas variantes para adecuar las respuestas institucionales al nuevo paradigma en salud mental comunitaria. Dicha adecuación se ve facilitada cuando las prácticas de salud mental se integran con las de salud, lo que impacta notablemente en la accesibilidad y calidad de los cuidados brindados. Se destaca la mayor complejidad de los abordajes implementados en los CAPS y en los centros de atención especializados en salud mental. En todos los efectores se plantean dificultades en la articulación entre los servicios de salud mental y los organismos de protección de los derechos de la niñez. Persisten obstáculos en la implementación de las normativas vigentes vinculados a procesos de capacitación de recursos humanos, abordajes interdisciplinarios y funcionamiento integral en la articulación intra e intersectorial en estrategias destinadas a la población infanto-juvenil.GT61: Políticas públicas y prácticas comunitarias en salud mental en Argentina. Debates y aportes al diálogo de saberes.Universidad Nacional de La Plat

Políticas de saúde mental e crianças na Argentina: nova normativa e lacunas na execução

Objetivos: caracterizar y evaluar la adecuación de las prácticas de cuidado que brindan los efectores del sistema público de salud mental infantil al nuevo marco normativo de Argentina. Materiales y Métodos: se trata de un diseño exploratorio descriptivo de casos múltiples, en los que se analizan 12 efectores de salud, a través de metodología cualitativa basada en el análisis documental y sistematización de entrevistas semiestructuradas aplicadas durante el año 2018, en las provincias de Santa Fe, La Rioja y Ciudad Autónoma de Buenos Aires. Resultados: en las jurisdicciones estudiadas se constató un proceso de reforma incipiente, aunque aún se detectan importantes obstáculos en la implementación en términos de capacitación de recursos humanos, financieros, bordajes interdisciplinarios y funcionamiento integral en redes intra e intersectoriales. En este proceso se destacan las diferencias entre los niveles de atención, aumentando la complejidad de los abordajes en Centros de Atención Primaria (CAPs) y Centros Especializados en Salud Mental (CESM). Conclusiones: la adecuación de las prácticas se vio facilitada en aquellas jurisdicciones donde los cuidados de salud mental se integran con la salud general, y donde existe una planificación central que asegura la accesibilidad y calidad de los cuidados brindados a partir de los recursos disponibles. Además, persisten dificultades en la articulación con organismos de protección de los derechos de la niñez.To characterize and evaluate the adequacy of the care practices provided by the public child mental health system to the new regulatory framework in Argentina. Materials and Methods: It is a descriptive exploratory design of multiple cases in which 12 health providers are analyzed through qualitative methodology based on documentary analysis and systematization of semi-structured interviews applied in 2018 in the provinces of Santa Fe, La Rioja and Ciudad Autónoma de Buenos Aires. Results: In the studied jurisdictions, an incipient reform process was observed, although significant obstacles in the implementation in terms of training of human and financial resources, interdisciplinary approaches and comprehensive operation in intra- and inter-sectoral networks are still being detected. Differences between the levels of care stand out in this process, increasing the complexity of the approaches in Primary Care Centers (PCCs) and Specialized Centers in Mental Health (SCMH). Conclusions: In the jurisdictions where mental health care is integrated with general health and where there is central planning that ensures the accessibility and quality of care provided based on available resources, the adaptation of practices was facilitated. In addition, difficulties persist in articulation with organizations for the protection of the rights of children.Objetivos: caracterizar e avaliar a adequação das práticas de cuidado que oferecem os efetores do sistema público de saúde mental infantil ao novo marco normativo da Argentina. Materiais e Métodos: trata-se de um desenho exploratório descritivo de casos múltiplos, nos que se analisam 12 efetores de saúde, a través de metodologia qualitativa baseada na análise documental e sistematização de entrevistas semiestruturadas feitas durante o ano 2018, nas províncias de Santa Fe, La Rioja e Cidade Autônoma de Buenos Aires. Resultados: nas jurisdições estudadas se verificou um processo de reforma incipiente, porém ainda se detectam importantes obstáculos na execução em termos de capacitação de recursos humanos, financeiros, abordagens interdisciplinares e funcionamento integral nas redes intra e intersetoriais. Neste processo se destacam diferenças entre os níveis de atenção, aumentando a complexidade das abordagens em Centros de Atenção Primaria (CAPs) e Centros Especializados em Saúde Mental (CESM). Conclusões: a adequação das práticas deu certo naquelas jurisdições onde os cidadãos de saúde mental se integram com a saúde geral, e onde existe uma planificação central que assegura a acessibilidade e qualidade dos cidadãos fornecidos a partir dos recursos disponíveis. Ainda, têm dificuldades na articulação com organismos de proteção dos direitos das crianças.Fil: Barcala, Alejandra Bibiana. Universidad Nacional de Lanús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Botto, Mercedes Isabel. Facultad Latinoamericana de Ciencias Sociales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Poverene, Laura Daniela. Universidad Nacional de Lanús; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Augsburger, Cecilia. Instituto de la Salud "juan Lazarte"; ArgentinaFil: Gerlero, Sandra Silvana. Instituto de la Salud "juan Lazarte"; ArgentinaFil: Saadi, Viviana. Universidad Nacional de La Rioja; ArgentinaFil: Nuñez, Alberto Gustavo. Universidad Nacional de Lanús; ArgentinaFil: Michalewicz, Alejandro. Universidad Nacional de Lanús; Argentin

Type-1 Cannabinoid Receptors Reduce Membrane Fluidity of Capacitated Boar Sperm by Impairing Their Activation by Bicarbonate

Background Mammalian spermatozoa acquire their full fertilizing ability (so called capacitation) within the female genital tract, where they are progressively exposed to inverse gradients of inhibiting and stimulating molecules. Methodology/Principal Findings In the present research, the effect on this process of anandamide, an endocannabinoid that can either activate or inhibit cannabinoid receptors depending on its concentration, and bicarbonate, an oviductal activatory molecule, was assessed, in order to study the role exerted by the type 1 cannabinoid receptor (CB1R) in the process of lipid membrane remodeling crucial to complete capacitation. To this aim, boar sperm were incubated in vitro under capacitating conditions (stimulated by bicarbonate) in the presence or in the absence of methanandamide (Met-AEA), a non-hydrolysable analogue of anandamide. The CB1R involvement was studied by using the specific inhibitor (SR141716) or mimicking its activation by adding a permeable cAMP analogue (8Br-cAMP). By an immunocytochemistry approach it was shown that the Met-AEA inhibits the bicarbonate-dependent translocation of CB1R from the post-equatorial to equatorial region of sperm head. In addition it was found that Met-AEA is able to prevent the bicarbonate-induced increase in membrane disorder and the cholesterol extraction, both preliminary to capacitation, acting through a CB1R-cAMP mediated pathway, as indicated by MC540 and filipin staining, EPR spectroscopy and biochemical analysis on whole membranes (CB1R activity) and on membrane enriched fraction (C/P content and anisotropy). Conclusions/Significance Altogether, these data demonstrate that the endocannabinoid system strongly inhibits the process of sperm capacitation, acting as membrane stabilizing agent, thus increasing the basic knowledge on capacitation-related signaling and potentially opening new perspectives in diagnostics and therapeutics of male infertility

Placental pathology in perinatal asphyxia: a case–control study

IntroductionPlacentas of term infants with birth asphyxia are reported to have more lesion such as maternal vascular malperfusion (MVM), fetal vascular malperfusion (FVM) and chorioamnionitis with fetal response (FIR) than those of term infants without birth asphyxia. We compared the placental pathology of asphyxiated newborns, including those who developed hypoxic-ischemic encephalopathy (HIE), with non-asphyxiated controls.MethodsWe conducted a retrospective case–control study of placentas from neonates with a gestational age ≥ 35 weeks, a birthweight ≥ 1,800 g, and no malformations. Cases were asphyxiated newborns (defined as those with an umbilical artery pH ≤ 7.0 or base excess ≤ −12 mMol, 10-minute Apgar score ≤ 5, or the need for resuscitation lasting >10 min) from a previous cohort, with (n=32) and without (n=173) diagnosis of HIE. Controls were non-asphyxiated newborns from low-risk l (n= 50) or high-risk (n= 68) pregnancies. Placentas were analyzed according to the Amsterdam Placental Workshop Group Consensus Statement 2014.ResultsCases had a higher prevalence of nulliparity, BMI>25, thick meconium, abnormal fetal heart monitoring, and acute intrapartum events than controls (p<0.001). MVM and FVM were more frequent among non-asphyxiated than asphyxiated newborns (p<0.001). There was no significant difference in inflammatory lesions or abnormal umbilical insertion site. Histologic meconium-associated changes (MAC) were observed in asphyxiated newborns only (p= 0.039).DiscussionOur results confirm the role of antepartum and intrapartum risk factors in neonatal asphyxia and HIE. No association between neonatal asphyxia and placental lesions was found, except for in the case of MAC. The association between clinical and placental data is crucial to understanding and possibly preventing perinatal asphyxia in subsequent pregnancies

Preceding anti-spike IgG levels predicted risk and severity of COVID-19 during the Omicron-dominant wave in Santa Fe city, Argentina

The SARS-CoV-2 Omicron variant has increased infectivity and immune escape compared with previous variants, and caused the surge of massive COVID-19 waves globally. Despite a vast majority (~90%) of the population of Santa Fe city, Argentina had been vaccinated and/or had been infected by SARS-CoV-2 when Omicron emerged, the epidemic wave that followed its arrival was by far the largest one experienced in the city. A serosurvey conducted prior to the arrival of Omicron allowed to assess the acquired humoral defences preceding the wave and to conduct a longitudinal study to provide individual-level real-world data linking antibody levels and protection against COVID-19 during the wave. A very large proportion of 1455 sampled individuals had immunological memory against COVID-19 at the arrival of Omicron (almost 90%), and about half (48.9%) had high anti-spike immunoglobulin G levels (>200 UI/ml). However, the antibody titres varied greatly among the participants, and such variability depended mainly on the vaccine platform received, on having had COVID-19 previously and on the number of days elapsed since last antigen exposure (vaccine shot or natural infection). A follow-up of 514 participants provided real-world evidence of antibody-mediated protection against COVID-19 during a period of high risk of exposure to an immune-escaping highly transmissible variant. Pre-wave antibody titres were strongly negatively associated with COVID-19 incidence and severity of symptoms during the wave. Also, receiving a vaccine shot during the follow-up period reduced the COVID-19 risk drastically (15-fold). These results highlight the importance of maintaining high defences through vaccination at times of high risk of exposure to immune-escaping variants.Fil: Eberhardt, María Ayelen Teresita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Simoncini, Melina Soledad. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Piña, Carlos Ignacio. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Galoppo, Germán Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Parachu Marco, Maria Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Racca, Andrea Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Arce, Sofía Irene. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Viotto, Evangelina del Valle. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Facelli Fernández, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Valli, Florencia Elizabeth. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Botto, Cecilia Cristina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Scarpa, Leonardo Javier. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Junges, Celina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Palavecino, Cintia Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Beccaría, Camila. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Sklar, Diego Mauricio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; ArgentinaFil: Mingo, Graciela Laura. Universidad Nacional de Entre Rios. Instituto de Estudios Sociales. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Estudios Sociales.; ArgentinaFil: Genolet, Alicia Susana Guadalupe. Universidad Nacional de Entre Rios. Instituto de Estudios Sociales. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Santa Fe. Instituto de Estudios Sociales.; ArgentinaFil: Muñoz de Toro, Monica Milagros. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Salud y Ambiente del Litoral. Universidad Nacional del Litoral. Instituto de Salud y Ambiente del Litoral; ArgentinaFil: Aimar, Hugo Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Matemática Aplicada del Litoral. Universidad Nacional del Litoral. Instituto de Matemática Aplicada del Litoral; ArgentinaFil: Martinez Marignac, Veronica Lucrecia. Provincia de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Universidad Autónoma de Entre Ríos. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Centro de Investigaciones Científicas y Transferencia de Tecnología a la Producción; ArgentinaFil: Bossio, Juan Carlos. Instituto Nacional de Enfermedades Respiratorias ‘Dr Emilio Coni’; ArgentinaFil: Armando, Gustavo. Instituto Nacional de Enfermedades Respiratorias ‘Dr Emilio Coni’; ArgentinaFil: Fernández, Hugo. Instituto Nacional de Enfermedades Respiratorias ‘Dr Emilio Coni’; ArgentinaFil: Beldomenico, Pablo Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentin

Survival of infants born with esophageal atresia among 24 international birth defects surveillance programs

Background: Esophageal atresia (EA) affects around 2.3–2.6 per 10,000 births world-wide. Infants born with this condition require surgical correction soon after birth. Most survival studies of infants with EA are locally or regionally based. We aimed to describe survival across multiple world regions. Methods: We included infants diagnosed with EA between 1980 and 2015 from 24 birth defects surveillance programs that are members of the International Clearinghouse for Birth Defects Surveillance and Research. We calculated survival as the proportion of liveborn infants alive at 1 month, 1- and 5-years, among all infants with EA, those with isolated EA, those with EA and additional anomalies or EA and a chromosomal anomaly or genetic syndrome. We also investigated trends in survival over the decades, 1980s–2010s. Results: We included 6,466 liveborn infants with EA. Survival was 89.4% (95% CI 88.1–90.5) at 1-month, 84.5% (95% CI 83.0–85.9) at 1-year and 82.7% (95% CI 81.2–84.2) at 5-years. One-month survival for infants with isolated EA (97.1%) was higher than for infants with additional anomalies (89.7%) or infants with chromosomal or genetic syndrome diagnoses (57.3%) with little change at 1- and 5-years. Survival at 1 month improved from the 1980s to the 2010s, by 6.5% for infants with isolated EA and by 21.5% for infants with EA and additional anomalies. Conclusions: Almost all infants with isolated EA survived to 5 years. Mortality was higher for infants with EA and an additional anomaly, including chromosomal or genetic syndromes. Survival improved from the 1980s, particularly for those with additional anomalies