160 research outputs found
Recent Advances in Adhesive Bonding - The Role of Biomolecules, Nanocompounds, and Bonding Strategies in Enhancing Resin Bonding to Dental Substrates
Purpose of review:
To present an overview on the main agents (i.e., biomolecules and nanocompounds) and/or strategies currently available to amplify or stabilize resin-dentin bonding.
Recent findings:
According to studies retrieved for full text reading (2014-2017), there are currently six major strategies available to overcome resin-dentin bond degradation: (i) use of collagen crosslinking agents, which may form stable covalent bonds with collagen fibrils, thus strengthening the hybrid layer; (ii) use of antioxidants, which may allow further polymerization reactions over time; (iii) use of protease inhibitors, which may inhibit or inactivate metalloproteinases; (iv) modification of the bonding procedure, which may be performed by using the ethanol wet-bonding technique or by applying an additional adhesive (hydrophobic) coating, thereby strengthening the hybrid layer; (v) laser treatment of the substrate prior to bonding, which may cause specific topographic changes in the surface of dental substrates, increasing bonding efficacy; and (vi) reinforcement of the resin matrix with inorganic fillers and/or remineralizing agents, which may positively enhance physico-mechanical properties of the hybrid layer.
Summary:
With the present review, we contributed to the better understanding of adhesion concepts and mechanisms of resin-dentin bond degradation, showing the current prospects available to solve that problematic. Also, adhesively-bonded restorations may be benefited by the use of some biomolecules, nanocompounds or alternative bonding strategies in order to minimize bond strength degradation
Glass-ceramic Flexural Strength after Hydrofluoric Acid and Unfilled Resin Treatment
poster abstractThe use of hydrofluoric (HF) acid is considered one of the most effective methods for achieving durable resin bond to glass-ceramics. Nonetheless, HF acid etching effect on glass-based ceramics strength remains uncertain and only a few contradictory studies have reported the influence of an unfilled resin (UR) application on the ceramic strength.
Objectives: To investigate the effect of HF acid etching followed by silane and UR applications on the biaxial flexural strength of a nanofluorapatite glass-ceramic.
Methods: 144 disc-shaped (15±1mm in diameter and 0.8±0.1mm in thickness) nanofluorapatite ceramic specimens were allocated into 12 groups: G1-control (no etching), G2-30s, G3-60s, G4-90s, G5-120s, G6-60s+60s. Meanwhile, G7-G12 were treated in the same fashion as G1-G6, but followed by silane and UR applications. Surface morphology of G1-G12 was assessed by scanning electron microscopy/SEM. The flexural strength was determined by biaxial testing per ISO 6872. Statistical analyses were two-way ANOVA and the Sidak multiple comparisons procedure (α=0.05). Additionally, Weibull statistics and finite element analysis (FEA) were carried out.
Results: A significant effect of etching time (p=0.0290) on flexural strength was observed. G4 led to a significantly (p=0.0392) higher flexural strength than G1. Correspondingly, G10 revealed a considerably higher flexural strength than G7 (p=0.0392). Furthermore, flexural strength was significantly higher for G7-G12 than for G1-G6 (p<0.0001). For G1-G6, G4 showed the highest Weibull characteristic strength and G10 also presented the highest Weibull characteristic strength among G7-G12. FEA showed lower stress concentration in G7-G12 with the gradient stress supporting the fracture types of the biaxial test. Finally, the SEM data revealed that the HF acid etching affected the surface of ceramic specimens by generating pores and irregularities and more importantly that the UR was able to penetrate into the ceramic microstructure.
Conclusions: HF acid etching followed by silane and UR applications enhanced the ceramic biaxial flexural strength
Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studies
OBJECTIVE:
A combination of antibiotics, including but not limited to metronidazole (MET) and ciprofloxacin (CIP), has been indicated to eradicate bacteria in necrotic immature permanent teeth prior to regenerative procedures. It has been shown clinically that antibiotic pastes may lead to substantial stem cell death. The aim of this study was to synthesise scaffolds containing various concentrations of CIP to enhance cell viability while preserving antimicrobial properties.
DESIGN:
Polydioxanone (PDS)-based electrospun scaffolds were processed with decreasing CIP concentrations (25-1 wt.%) and morphologically evaluated using scanning electron microscopy (SEM). Cytotoxicity assays were performed to determine whether the amount of CIP released from the scaffolds would lead to human dental pulp stem cell (hDPSC) toxicity. Similarly, WST-1 assays were performed to evaluate the impact of CIP release on hDPSC proliferation. Pure PDS scaffolds and saturated double antibiotic solution MET/CIP (DAP) served as both positive and negative controls, respectively. Antibacterial efficacy against E. faecalis (Ef) was tested.
RESULTS:
A significant decrease in hDPSC' viability at concentrations 5-25 wt.% was observed. However, concentrations below 5wt.% did not impair cell viability. Data from the WST-1 assays indicated no detrimental impact on cell proliferation for scaffolds containing 2.5 wt.% CIP or less. Significant antimicrobial properties were seen for CIP-scaffolds at lower concentrations (i.e., 1 and 2.5 wt.%).
CONCLUSION:
The obtained data demonstrated that a reduced concentration of CIP incorporated into PDS-based scaffolds maintains its antimicrobial properties while enhancing viability and proliferation of dental pulp stem cells
Antimicrobial Efficacy of Triple Antibiotic-Eluting Polymer Nanofibers against Multispecies Biofilm
The elimination of microbial flora in cases of immature permanent teeth with necrotic pulp is both key and a challenging goal for the long-term success of regenerative therapy. Recent research has focused on the development of cell-friendly intracanal drug delivery systems. This in vitro study aimed to investigate the antimicrobial action of 3-dimensional (3D) tubular-shaped triple antibiotic-eluting nanofibrous constructs against a multispecies biofilm on human dentin. Polydioxanone polymer solutions, antibiotic-free or incorporated with metronidazole, ciprofloxacin, and minocycline, were electrospun into 3D tubular-shaped constructs. A multispecies biofilm consisting of Actinomyces naeslundii, Streptococcus sanguinis, and Enterococcus faecalis was forced inside the dentinal tubules via centrifugation in a dentin slice in vitro model. The infected specimens were exposed to 2 experimental groups (ie, 3D tubular-shaped triple antibiotic-eluting constructs and triple antibiotic paste [TAP]) and 2 control groups (7-day biofilm untreated and antibiotic-free 3D tubular-shaped constructs). Biofilm elimination was quantitatively analyzed with confocal laser scanning microscopy. Confocal laser scanning microscopic (CLSM) analysis showed a dense population of viable (green) bacteria adhered to dentin and penetrated into the dentinal tubules. Upon 3D tubular-shaped triple antibiotic-eluting nanofibrous construct exposure, nearly complete elimination of viable bacteria on the dentin surface and inside the dentinal tubules was shown in the CLSM images, which was similar (P < .05) to the bacterial death promoted by the TAP group but significantly greater when compared with both the antibiotic-free 3D tubular-shaped constructs and the control (saline). The proposed 3D tubular-shaped antibiotic-eluting construct showed pronounced antimicrobial effects against the multispecies biofilm tested and therefore holds significant clinical potential as a disinfection strategy before regenerative endodontics
Advanced Scaffolds for Dental Pulp and Periodontal Regeneration
No current therapy promotes root canal disinfection and regeneration of the pulp-dentin complex in cases of pulp necrosis. Antibiotic pastes used to eradicate canal infection negatively affect stem cell survival. Three-dimensional easy-to-fit antibiotic-eluting nanofibers, combined with injectable scaffolds, enriched or not with stem cells and/or growth factors, may increase the likelihood of achieving predictable dental pulp regeneration. Periodontitis is an aggressive disease that impairs the integrity of tooth-supporting structures and may lead to tooth loss. The latest advances in membrane biomodification to endow needed functionalities and technologies to engineer patient-specific membranes/constructs to amplify periodontal regeneration are presented
The Effect of Polymerization Methods and Fiber Types on the Mechanical Behavior of Fiber-Reinforced Resin-Based Composites
Purpose
Glass fibers were introduced to increase the fracture resistance of resin-based composites restorations; however, the poor polymerization between fibers and resin-based composite were sometimes noted and can cause debonding and failure. The purpose of this study was to investigate the effects of different polymerization methods as well as fiber types on the mechanical behavior of fiber-reinforced resin-based composites.
Materials and Methods
Seventy-five specimens were fabricated and divided into one control group and four experimental groups (n = 15), according to the type of glass fiber (strip or mesh) and polymerization methods (one- or two-step). A 0.2-mm-thick fiber layer was fabricated with different polymerization methods, on top of which a 1.8 mm resin-based composite layer was added to make a bar-shape specimen, followed by a final polymerization. Specimens were tested for flexural strength and flexural modulus. The failure modes of specimens were observed by scanning electron microscopy.
Results
The fiber types showed significant effect on the flexural strength of test specimens (F = 469.48, p < 0.05), but the polymerization methods had no significant effect (F = 0.05, p = 0.82). The interaction between these two variables was not significant (F = 1.73, p = 0.19). In addition, both fiber type (F = 9.71, p < 0.05) and polymerization method (F = 12.17, p < 0.05) affected the flexural modulus of test specimens; however, the interaction between these two variables was not significant (F = 0.40, p = 0.53).
Conclusions
The strip fibers showed better mechanical behavior than mesh fibers and were suggested for resin-based composites restorations reinforcement; however, different polymerization methods did not have a significant effect on the strength and failure mode of fiber-reinforced resin-based composites
Stem cell-derived tissue-engineered constructs for hemilaryngeal reconstruction
OBJECTIVES: As an initial step toward our goal of developing a completely tissue-engineered larynx, the aim of this study was to describe and compare three strategies of creating tissue-engineered muscle-polymer constructs for hemilaryngeal reconstruction.
METHODS: Cartilage-mimicking polymer was developed from electrospun poly(D,L-lactide-co-Δ-caprolactone) (PCL). Primary muscle progenitor cell cultures were derived from syngeneic F344 rat skeletal muscle biopsies. Twenty F344 rats underwent resection of the outer hemilaryngeal cartilage with the underlying laryngeal adductor muscle. The defects were repaired with muscle stem cell-derived muscle-PCL constructs (5 animals), myotube-derived muscle-PCL constructs (5 animals), motor end plate-expressing muscle-PCL constructs (5 animals), or PCL alone (controls; 5 animals). The outcome measures at 1 month included animal survival, muscle thickness, and innervation status as determined by electromyography and immunohistochemistry.
RESULTS: All of the animals survived the 1-month implant period and had appropriate weight gain. The group that received motor end plate-expressing muscle-PCL constructs demonstrated the greatest muscle thickness and the strongest innervation, according to electromyographic activity and the percentage of motor end plates that had nerve contact.
CONCLUSIONS: Although all of the tissue-engineered constructs provided effective reconstruction, those that expressed motor end plates before implantation yielded muscle that was more strongly innervated and viable. This finding suggests that this novel approach may be useful in the development of a tissue-engineered laryngeal replacement
Physicochemical and biological properties of novel chlorhexidineâloaded nanotubeâmodified dentin adhesive
A commercially available threeâstep (etchâandârinse) adhesive was modified by adding chlorhexidine (CHX)âloaded nanotubes (HalloysiteÂź, HNT) at two concentrations (CHX10% and CHX20%). The experimental groups were: SBMP (unmodified adhesive, control), HNT (SBMP modified with HNT), CHX10 (SBMP modified with HNT loaded with CHX10%), and CHX20 (SBMP modified with HNT loaded with CHX20%). Changes in the degree of conversion (DC%), Knoop hardness (KHN), water sorption (WS), solubility (SL), antimicrobial activity, cytotoxicity, and antiâmatrix metalloproteinase [MMPâ1] activity (collagenaseâI) were evaluated. In regards to DC%, twoâway ANOVA followed by Tukeyâs postâhoc test revealed that only the factor âadhesiveâ was statistically significant (p 0.05). For Knoop microhardness, oneâway ANOVA followed by the Tukeyâs test showed statistically significant differences when comparing HNT (20.82 ± 1.65) and CHX20% (21.71 ± 2.83) with the SBMP and CHX10% groups. All adhesives presented similar WS and cytocompatibility. The CHXâloaded nanotubeâmodified adhesive released enough CHX to inhibit the growth of S. mutans and L. casei. Adhesive eluates were not able to effectively inhibit MMPâ1 activity. The evaluation of higher CHX concentrations might be necessary to provide an effective and predictable MMP inhibition. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res B Part B, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 868â875, 2019.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/148225/1/jbmb34183_am.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/148225/2/jbmb34183.pd
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm
OBJECTIVES:
This study sought to investigate, in vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis-infected dentin biofilm.
MATERIALS AND METHODS:
Dentin specimens (4 Ă 4 Ă 1 mm(3)) were prepared from human canines. The specimens were sterilized, inoculated with P. gingivalis (ATCC 33277), and incubated for 1 week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS + 25 wt% TAP [25 mg of each antibiotic (metronidazole, ciprofloxacin, and minocycline) per mL of the PDS polymer solution], or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU) per milliliter (n = 10/group) measurement was performed. Furthermore, additional specimens (n = 2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed, and significance was set at the 5% level.
RESULTS:
Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p < 0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU per milliliter data between antibiotic-free scaffolds (2.7 log10 CFU/mL) and the negative control (5.9 log10 CFU/mL).
CONCLUSIONS:
The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established P. gingivalis-infected dentin biofilm.
CLINICAL RELEVANCE:
Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics
Comparison of Internal Adaptation of Bulk-fill and Increment-fill Resin Composite Materials
Objectives: To evaluate 1) the internal adaptation of a light-activated incremental-fill and bulk-fill resin-based composite (RBC) materials by measuring the gap between the restorative material and the tooth structure and 2) the aging effect on internal adaptation.
Methods and Materials: Seventy teeth with class I cavity preparations were randomly distributed into five groups; four groups were restored with bulk-fill RBCs: Tetric EvoCeram Bulk Fill (TEC), SonicFill (SF), QuiXX Posterior Restorative (QX), and X-tra fil (XF); the fifth group was restored with incremental-fill Filtek Supreme Ultra Universal Restorative (FSU). One-half of the specimens of each group were thermocycled. Each tooth was sectioned, digital images were recorded, and the dimensions of any existing gaps were measured. Data were analyzed using analysis of variance (α=0.05).
Results: FSU had the smallest gap measurement values compared with the bulk-fill materials except QX and TEC (pâ€0.008). FSU had the smallest sum of all gap category values compared with the bulk-fill materials, except QX (pâ€0.021). The highest gap incidence and size values were found at the composite/adhesive interface. All aged groups had greater gap values in regard to the gap measurement and the sum of all gap categories compared with non-aged groups.
Significance: The incrementally placed material FSU had the highest internal adaptation to the cavity surface, while the four bulk-fill materials showed varied results. Thermocycling influenced the existing gap area magnitudes. The findings suggest that the incremental-fill technique produces better internal adaptation than the bulk-fill technique
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