ESCOLA TÉCNICA DE SAÚDE: INICIAÇÃO CIENTÍFICA E ENSINO REMOTO EMERGENCIAL

This article seeks to present and discuss some aspects of the experience of carrying out Emergency Remote Teaching (ERE) in the pandemic context in a technical health school, focusing on the institutional responses to new demands and challenges, as well as the analysis of construction of course conclusion works linked to the scientific initiation of integrated high school courses. A qualitative approach was used as a methodology for the preparation of the article, accompanied by a literature review and monitoring documents of the ERE implementation process. The results show that, although it is possible to make positive evaluations with the resumption of activities, the ERE remote format showed limits, especially regarding the pedagogical proposal (schedule, schedules, contents, balance between classes and asynchronous work) and the homeization of teaching. in extreme life contexts and even more vulnerable by the pandemic.Este artigo busca apresentar e debater alguns aspectos da experiência de realização do Ensino Remoto Emergencial (ERE) no contexto pandêmico em uma escola técnica de saúde, tendo como foco de análise as respostas institucionais às novas demandas e desafios, assim como, a análise de construção de trabalhos de conclusão de curso vinculados à iniciação científica de cursos de ensino médio integrado. Foi utilizada como metodologia para a confecção do artigo uma abordagem qualitativa acompanhada de revisão bibliográfica e de documentos de monitoramento do processo de implementação do ERE. Os resultados apontam que, embora seja possível tecer avaliações positivas com a retomada de atividades, o formato remoto o ERE evidenciou limites, especialmente quanto a proposta pedagógica (calendário, grades, conteúdos, equilíbrio entre as aulas e trabalhos assíncronos) e a domicialirização do ensino em contextos de vida extremos e ainda mais vulnerabilizados pela pandemia

Effect of UV-C Radiation on Shelf life of Vacuum Package Colossoma Macropomum x Piaractus Mesopotamicus Fillets

AbstractColossoma macropomum x Piaractus mesopotamicus (CP) is a freshwater fish with greatest commercial importance in Brazil. Fillets of CP are highly perishable food and preservation technology with UV-C could improve food safety and extend shelf life. Fillet samples were submitted at UV-C (55.83mJ/cm2) and examined for mesophilic and psychrotrophic count and biogenic amines over 6 days. UV-C reduced the bacterial growth and number of colonies in the stationary phase; also increase the levels of cadaverine, putrescine and histamine. The results suggest that UV-C enhanced the shelf-life of CP fillets by at least 50%

Prevalence of hepatitis E virus RNA and antibodies in a cohort of kidney transplant recipients in Central Brazil

Submitted by Sandra Infurna ([email protected]) on 2018-06-12T13:14:29Z No. of bitstreams: 1 andresa_lemos_etal_IOC_2017.pdf: 287595 bytes, checksum: e3f18737eed7adedbd55482898af3e1e (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2018-06-12T13:21:05Z (GMT) No. of bitstreams: 1 andresa_lemos_etal_IOC_2017.pdf: 287595 bytes, checksum: e3f18737eed7adedbd55482898af3e1e (MD5)Made available in DSpace on 2018-06-12T13:21:05Z (GMT). No. of bitstreams: 1 andresa_lemos_etal_IOC_2017.pdf: 287595 bytes, checksum: e3f18737eed7adedbd55482898af3e1e (MD5) Previous issue date: 2018Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.Universidade de Goiás. Faculdade de Enfermagem. Goiânia, GO, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, GO, Brasil.To assess the prevalence of hepatitis E virus (HEV) RNA and antibodies among kidney transplant recipients (KTR) in Central Brazil. The presence of chronic HEV infection was also investigated

Influence of Insertion Techniques for Resin Cement and Mechanical Cycling on the Bond Strength Between Fiber Posts and Root Dentin

Purpose: To evaluate the effect of the insertion technique for resin cement and mechanical cycling on the bond strength between fiber posts and root dentin.Materials and Methods: Sixty-four single-rooted bovine teeth were endodontically prepared to receive glass-fiber posts. The insertion of cement into the root canal was performed using one of the following techniques: POS, insertion with the post; LEN, the use of a lentulo-type drill; EXP, insertion with a straight-tip explorer; or CEN, the use of a Centrix syringe. Half of the specimens were mechanically cycled. All specimens were sectioned into slices of 1.8 mm for the push-out test and 0.5 mm for analysis of the cement layer quality.Results: The insertion technique affected the interaction between factors (bond strength and mechanical cycling; p < 0.0001). Insertion of the Centrix syringe after mechanical cycling showed the highest bond values (13.6 +/- 3.2 MPa). Group-to-group comparisons for baseline and cycled conditions indicated that mechanical cycling significantly influenced the bond strength (p < 0.0001) of the POS and CEN groups. The quality of the cement layer did not differ between the techniques when evaluated in the middle (p = 0.0612) and cervical (p = 0.1119) regions, but did differ in the apical region (p = 0.0097), where the CEN group had better layer quality for the two conditions tested (baseline and cycled).Conclusion: The use of the Centrix syringe improved the homogeneity of the cement layer, reducing the defects in the layer and increasing adhesive strength values to dentin, even after mechanical cycling

Cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with hepatitis E virus: The bone marrow as a possible new viral target.

Hepatitis E virus (HEV) transmission through infected blood and blood products has already been described. However, little is known about the bone marrow (BM) as source of HEV infection. Our study aimed to investigate the presence of HEV antigen (Ag) and histological changes in BM of cynomolgus monkeys (Macaca fascicularis) experimentally and naturally infected with HEV. Four cynomolgus monkeys with acute, and two with chronic hepatitis E ─ after immunosuppressive therapy with tacrolimus ─ were compared with one colony-bred animal naturally infected. Both, natural and experimental infections were characterized by anti-HEV IgG seroconversion detected by ELISA, and viral RNA isolation confirmed by RT-qPCR and qualitative nested RT-PCR. BM biopsies were collected from all animals, submitted to histology and indirect immunofluorescence techniques and observed, respectively, by light and confocal microscopy. The HEV Ag-fluorescent-labeled cells were detected from BM biopsies obtained from three monkeys with acute and one with chronic hepatitis E, and also from the naturally infected monkey. In the experimentally infected animals with acute hepatitis, HEV Ag detection occurred at 160 days post-infection, even after viral clearance in serum, feces, and liver. Double-stranded RNA, a replicative marker, was detected in BM cells from both acute and chronically infected animals. Major histological findings included vacuolization in mononuclear and endosteal cells, an absence of organized inflammatory infiltrates, and also some fields suggesting displasic focal BM disease. These findings support the hypothesis of BM cells as secondary target sites of HEV persistence. Further experimental studies should be carried out to confirm the assumption of HEV transmission through BM transplantation

Evidences of HEV genotype 3 persistence and reactivity in liver parenchyma from experimentally infected cynomolgus monkeys (Macaca fascicularis).

Hepatitis E virus genotype 3 (HEV-3) is an emerging zoonotic pathogen, responsible for sporadic cases of acute hepatitis E worldwide. Primate models have proven to be an essential tool for the study of HEV pathogenesis. Here we describe the outcomes of HEV infection in Macaca fascicularis (cynomolgus) inoculated experimentally with genotype 3. Eight adult cynomolgus macaques were inoculated intravenously with HEV-3 viral particles isolated from swine and human samples. Liver, spleen, duodenum, gallbladder and bile were sequential assessed up to the end-point of this study, 67 days post-inoculation (dpi). Our previously published findings showed that biochemical parameters return gradually to baseline levels at 55 dpi, whereas anti-HEV IgM and HEV RNA become undetectable in the serum and feces of all animals, indicating a non-viremic phase of recovery. Nevertheless, at a later stage during convalescence (67 dpi), the presence of HEV-3 RNA and antigen persist in central organs, even after peripheral viral clearance. Our results show that two cynomolgus inoculated with swine HEV-3 (animals I3 and O1) presented persistence of HEV RNA low titers in liver, gallbladder and bile. At this same stage of infection, HEV antigen (HEV Ag) could be detected in all infected animals, predominantly in non-reactive Kupffer cells (CD68+iNOS-) and sinusoidal lining cells. Simultaneously, CD4+, CD3+CD4+, and CD3+CD8+ immune cells were identified in hepatic sinusoids and small inflammatory clusters of lobular mononuclear cells, at the end-point of this study. Inability of HEV clearance in humans can result in chronic hepatitis, liver cirrhosis, with subsequent liver failure requiring transplantation. The results of our study support the persistence of HEV-3 during convalescence at 67 dpi, with active immune response in NHP. We alert to the inherent risk of viral transmission through liver transplantation, even in the absence of clinical and biochemical signs of acute infection. Thus, besides checking conventional serological markers of HEV infection, we strongly recommend HEV-3 RNA and antigen detection in liver explants as public health measure to prevent donor-recipient transmission and spread of hepatitis E