Vrae oor Christelike wetenskap

Die besinning oor Christelike wetenskap gaan nog steeds voort. Hierdie uitgawe is daar weer eens bewys van. Die feit van die voortdurende besinning sou op verskillende wyses geinterpreteer kon word: enersyds as ’n aanklag dat daar nog steeds nie helderheid bestaan oor hierdie belangrike saak nie; andersyds as ’n teken van ’n lewenskragtige besinning oor ’n tema wat in feite nooit uitgeput kan word nie

Numerical simulations of sunspots

The origin, structure and evolution of sunspots are investigated using a numerical model. The compressible MHD equations are solved with physical parameter values that approximate the top layer of the solar convection zone. A three dimensional (3D) numerical code is used to solve the set of equations in cylindrical geometry, with the numerical domain in the form of a wedge. The linear evolution of the 3D solution is studied by perturbing an axisymmetric solution in the azimuthal direction. Steady and oscillating linear modes are obtained

Towards reconciliation of structure with function in plasmodesmata—who is the gatekeeper?

Whilst the structure of higher plant plasmodesmata was first described by Robards (1963. Desmotubule—a plasmodesmatal substructure. Nature 218, 784), and despite many subsequent intensive investigations, there is still much that remains unclear relating to their ultrastructure and functioning in higher plants. We have examined chemically fixed plant material, and suggest that the conformational changes seen in plasmodesmatal substructure, particularly the deposition of electron-dense extra-plasmodesmal material, is linked to either manipulation of the hormonal balance (as in Avocado fruit), or of osmotic potential in leaf blade material. These changes result in the deposition of β 1,3-glucan (callose) at the neck region of these plasmodesmata. This electron-dense material is deposited at the neck region of plasmodesmata, and forms a collar-like structure. The formation of a collar is shown to be coupled with loss of lucence within the cytoplasmic sleeve. The formation of a collar at the plasmodesmatal orifice thus results in encapsulation and closure of the plasmodesmatal orifice. Closure of the orifice coincides with a loss of electron-lucence and a lack of resolution of the desmotubule. These ultrastructural changes are potentially significant and could contribute to, result in, or assist in the down-regulation of cell to cell trafficking via plasmodesmata

Plasmodesmatal frequency in relation to short-distance transport and phloem loading in leaves of barley (Hordeum vulgare). Phloem is not loaded directly from the symplast

We investigated the phloem loading pathway in barley, by determining plasmodesmatal frequencies at the electron microscope level for both intermediate and small blade bundles of mature barley leaves. Lucifer yellow was injected intercellularly into bundle sheath, vascular parenchyma, and thin-walled sieve tubes. Passage of this symplastically transported dye was monitored with an epifluorescence microscope under blue light. Low plasmodesmatal frequencies endarch to the bundle sheath cells are relatively low for most interfaces terminating at the thin- and thick-walled sieve tubes within this C3 species. Lack of connections between vascular parenchyma and sieve tubes, and low frequencies (0.5% plasmodesmata per μm cell wall interface) of connections between vascular parenchyma and companion cells, as well as the very low frequency of pore-plasmodesmatal connections between companion cells and sieve tubes in small bundles (0.2% plasmodesmata per μm cell wall interface), suggest that the companion cell-sieve tube complex is symplastically isolated from other vascular parenchyma cells in small bundles. The degree of cellular connectivity and the potential isolation of the companion cell-sieve tube complex was determined electrophysiologically, using an electrometer coupled to microcapillary electrodes. The less negative cell potential {average -52 mV) from mesophyll to the vascular parenchyma cells contrasted sharply with the more negative potential (-122.5 mV) recorded for the companion cell-thin-walled sieve tube complex. Although intercellular injection of lucifer yellow clearly demonstrated rapid (0.75 μm s-1) longitudinal and radial transport in the bundle sheath-vascular parenchyma complex, as well as from the bundle sheath through transverse veins to adjacent longitudinal veins, we were neither able to detect nor present unequivocal evidence in support of the symplastic connectivity of the sieve tubes to the vascular parenchyma. Injection of the companion cell-sieve tube complex, did not demonstrate backward connectivity to the bundle sheath. We conclude that the low plasmodesmatal frequencies, coupled with a two-domain electropotential zonation configuration, and the negative transport experiments using lucifer yellow, precludes symplastic phloem loading in barley leaves

OBSERVATIONS ON THE ECOLOGY OF PLEUROBRACHIA PILEUS (CTENOPHORA) IN THE SOUTHERN BENGUELA ECOSYSTEM

The vertical distribution, abundance and size composition of a population of Pleurobrachia pileus was studied during a six-day drogue study conducted off the west coast of South Africa in February 1995. The population was centred in deep water, possibly because of the presence of a potential predator, Beroe sp., at shallower depths. The population of P. pileus failed to display clear patterns of migration, although nocturnal recruitment from deeper water resulted in a deepening of the depth of occupation then. The population was dominated by individual

The microstructure of plasmodesmata in internodal stem tissue of the Saccharum hybrid var. NCo376 : evidence for an apoplasmic loading pathway

The distribution, structure and functional state of plasmodesmata were investigated to gain a clearer understanding of the sucrose transport pathway to the storage parenchyma cells in stem tissue in Saccharum officinarum var. NCo376. Evidence from structural studies on sugarcane stems by electron microscopy indicated that there are numerous plasmodesmata from the vascular bundles through to the storage parenchyma cells in mature stem tissue. Our studies, supported by fluorescence microscopy and iontophoresis, indicate that there are functional plasmodesmata in the phloemunloading pathway from transport phloem tissue to the bundle sheath in Saccharum, which could support symplasmic transport; plasmodesmata outside of the sheath cells in the storage parenchyma appear to be constricted by sphincter-like structures within their neck regions. Staining with Aniline Blue revealed evidence of large callose deposits, which co-localized with plasmodesmatal aggregates in the walls of the storage parenchyma cells. This suggests that the sucrose transport into, and accumulation by, storage parenchyma of mature stem tissue is under apoplasmic control

Russian wheat aphid causes greater reduction in phloem transport capacity of barley leaves than bird cherry-oat aphid

The effects of feeding by the Russian wheat aphid (RWA), Diuraphis noxia Mordvilko and the Bird cherry-oat aphid (BCA), Rhopalosiphum padi L on the transport capacity of barley Hordeum vulgare L leaves were investigated and compared with a view to relating these effects to the visible symptoms shown by the respective infested plants. RWAcauses extensive chlorosis and necrosis on an infested plant whereas BCA causes no observable symptoms. Our results using the xenobiotic, phloem mobile fluorophore, 5, 6 carboxyfluorescein diacetate (5, 6-CFDA) revealed striking differences in damage to the transport of assimilates through the phloem by these two aphids. The result clearly suggests that short-term feeding by RWA causes a reduction in transport of assimilates and a more severe reduction or perhaps even permanent cessation of transport during long-term feeding. In contrast, feeding by BCA does not lead to a marked decrease in transport during short-term feeding period, however, a reduction in the transport was recorded during long-term feeding activities. These results perhaps suggest that damage to transport capacities of the barley leaves appears to be partly responsible for the observed symptoms in RWA-infested plants and the lack of them during BCAinfestations, symptoms such as reduction or cessation in transport of assimilates to growing tissues may lead to such observable symptoms