The Distribution, Natural History and the Conservation Status of Batrachostomus Moniliger (Aves: Podargidae) in Last Two Decades from Sri Lanka

The Sri Lanka frogmouth (Batrachostomus moniliger) is a small-sized nocturnal bird largely restricted to tropical lowland forests with thick undergrowth. The motionless roosting posture as well as their plumage coloration and color patterns (which resemble that of tree stems and branches) enable them to camouflage and thereby avoid predation. Through opportunistic field surveys over a 20-year period (January 1998-February 2018), we surveyed different bioclimatic regions of Sri Lanka covering 500 sites, and documented presence of Sri Lankan frogmouth in 83 of sites. In these 83 sites, we recorded 136 birds including seven nesting pairs and chicks. Our survey confirmed frogmouth presence in four floristic regions of Sri Lanka, particularly from lower elevations (11-767 m). Most sightings were made in lowland rainforests while savannah woodlands had the least number of records; no frogmouths were recorded inside anthropocentric land-cover types such as commercial-scale farmlands or plantations. According to the habitat suitability model we constructed (MaxEnt-based), much of the lowland wet zone, particularly the southwestern corner, was predicted as the most suitable areas for Sri Lankan Frogmouth while the northeastern coastal plains, and the mid-western and northwestern parts of Sri Lanka seemed least suitable. According to The Maxent model’s internal jackknife test of variable importance, temperature seasonality is the most important predictor of frogmouth’s distribution. Sri Lanka frogmouths have a high fidelity for their roosting sites as they remained in the same tree at least for a week; preferred roosting trees were medium-sized Dicot species with a dense canopy cover and variable canopy heights. These roosting sites are relatively cool, humid with little exposure to direct sunlight. Both roosting and nesting trees were relatively isolated from neighboring canopy trees, thus, the understory surrounding the roosting tree was dense. Six of the nesting sites observed were located in the lowland wet zone rainforests while the other was in savannah woodlands of the intermediate zone. These nests were positioned approximately 66% of maximum canopy height of the host tree. Nests were constructed on relatively thin branches that formed an acute angle against the main stem. These nests are shallow, circular-shaped pads. The nest interior was cushioned with cotton, parts of fishtail palm, and down feathers while the nest exterior contained pieces of lichens and tree bark. Sri Lanka Frogmouth’s home ranges appeared to be very small in nesting season, a maximum of 60 m radius area around the roosting site. Both male and female birds alternate nest-guarding duties through most of the night-time. The major threat for the frogmouth in Sri Lanka includes habitat loss due to expansion of commercial-scale agriculture and monoculture plantations, illicit forest encroachments, and clear-cutting.Keywords: Ecology, Frogmouth, Habitat modeling, Nesting, Threat

Two new species of the genus Sitana Cuvier, 1829 (Reptilia: Agamidae) from Sri Lanka, including a taxonomic revision of the Indian Sitana species

Thasun Amarasinghe, A. A., Ineich, Ivan, Suranjan Karunarathna, D. M. S., Botejue, W. Madhava S., Campbell, Patrick D. (2015): Two new species of the genus Sitana Cuvier, 1829 (Reptilia: Agamidae) from Sri Lanka, including a taxonomic revision of the Indian Sitana species. Zootaxa 3915 (1): 67-98, DOI: http://dx.doi.org/10.11646/zootaxa.3915.1.

Avifaunal diversity in the peripheral areas of the Maduruoya National Park in Sri Lanka: With conservation and management implications

A survey was randomly conducted in the marginal areas of Maduruoya National Park, Sri Lanka for a period of > 7 years. These study sites are located within the dry zone and the intermediate zone. The main vegetation type of the area is dry mixed evergreen forest. We recorded 196 bird species belonging to 66 families, and they included 161 breeding residents, 25 purely migrants, nine both resident and migrants, one vagrant, 14 nationally threatened, three globally threatened, and 10 endemic species. We also report the first-ever records of Chestnut-backed Owlet, Red-faced Malkoha, and Spot-winged Thrush from this dry area. However, these precious habitats and its species are threatened because of irresponsible human activities such as forest fires, land filings, hunting, road kills, encroachments, garbage dumping, agrochemicals, granite-rock blasting, logging, and road constructions. Therefore, we recommend that relevant authorities take immediate conservation action to increase the protection of these marginal areas or buffer zone in the near future

Description of a new species of the genus Cnemaspis Strauch, 1887 (Reptilia: Squamata: Gekkonidae) from the Nilgala Savannah forest, <br />Uva Province of Sri Lanka

Karunarathna, Suranjan, Bauer, Aaron M., Silva, Anslem De, Surasinghe, Thilina, Somaratna, Lankani, Madawala, Majintha, Gabadage, Dinesh, Botejue, Madhava, Henkanaththegedara, Sujan, Ukuwela, Kanishka D.B. (2019): Description of a new species of the genus Cnemaspis Strauch, 1887 (Reptilia: Squamata: Gekkonidae) from the Nilgala Savannah forest, Uva Province of Sri Lanka. Zootaxa 4545 (3): 389-407, DOI: https://doi.org/10.11646/zootaxa.4545.3.

Biocatalytic Conversion of Avermectin to 4″-Oxo-Avermectin: Improvement of Cytochrome P450 Monooxygenase Specificity by Directed Evolution▿ †

Discovery of the CYP107Z subfamily of cytochrome P450 oxidases (CYPs) led to an alternative biocatalytic synthesis of 4″-oxo-avermectin, a key intermediate for the commercial production of the semisynthetic insecticide emamectin. However, under industrial process conditions, these wild-type CYPs showed lower yields due to side product formation. Molecular evolution employing GeneReassembly was used to improve the regiospecificity of these enzymes by a combination of random mutagenesis, protein structure-guided site-directed mutagenesis, and recombination of multiple natural and synthetic CYP107Z gene fragments. To assess the specificity of CYP mutants, a miniaturized, whole-cell biocatalytic reaction system that allowed high-throughput screening of large numbers of variants was developed. In an iterative process consisting of four successive rounds of GeneReassembly evolution, enzyme variants with significantly improved specificity for the production of 4″-oxo-avermectin were identified; these variants could be employed for a more economical industrial biocatalytic process to manufacture emamectin