Response of marine and freshwater algae to nitric acid and elevated carbon dioxide levels simulating environmental effects of bolide impact

One of the intriguing facets of the Cretaceous-Tertiary extinction is the apparently selective pattern of mortality amongst taxa. Some groups of organisms were severely affected and some remained relatively unscathed as they went through the K/T boundary. While there is argument concerning the exact interpretation of the fossil record, one of the best documented extinctions at the Cretaceous-Tertiary boundary is that of the calcareous nannoplankton. These organisms include coccolithic algae and foraminiferans. Attempts to explain their decline at the K/T boundary center around chemistry which could affect their calcium carbonate shells while leaving their silica-shelled cousins less affected or unaffected. Two environmental consequences of an extraterrestrial body impact which were suggested are the production of large quantities of nitrogen oxides generated by the shock heating of the atmosphere and the possible rise in CO2 from the dissolution of CaCO3 shells. Both of these phenomena would acidify the upper layers of the oceans and bodies of freshwater not otherwise buffered. The effects of nitric acid, carbon dioxide, or both factors on the growth and reproduction of calcareous marine coccoliths and non-calcareous marine and freshwater species of algae were considered. These experiments demonstrate that nitric acid and carbon dioxide have significant effects on important aspects of the physiology and reproduction of modern algae representative of extinct taxa thought to have suffered significant declines at the Cretaceous-Tertiary boundary. Furthermore, calcareous species showed more marked effects than siliceous species and marine species tested were more sensitive than freshwater species

Subsurface microbial habitats on Mars

We developed scenarios for shallow and deep subsurface cryptic niches for microbial life on Mars. Such habitats could have considerably prolonged the persistence of life on Mars as surface conditions became increasingly inhospitable. The scenarios rely on geothermal hot spots existing below the near or deep subsurface of Mars. Recent advances in the comparatively new field of deep subsurface microbiology have revealed previously unsuspected rich aerobic and anaerobic microbal communities far below the surface of the Earth. Such habitats, protected from the grim surface conditions on Mars, could receive warmth from below and maintain water in its liquid state. In addition, geothermally or volcanically reduced gases percolating from below through a microbiologically active zone could provide the reducing power needed for a closed or semi-closed microbial ecosystem to thrive

Humphrey Center News: Fall 1989 v. 4, no. 2

Newsletter of the Hubert H. Humphrey Cancer Research Center at Boston University School of Medicine

Humphrey Center News: Spring 1990 v. 5, no. 1

Newsletter of the Hubert H. Humphrey Cancer Research Center at Boston University School of Medicine

Humphrey Center News: Spring 1991 v. 6, no. 1

Newsletter of the Hubert H. Humphrey Cancer Research Center at Boston University School of Medicine

Humphrey Center News: Winter 1990 v. 5, no. 2

Newsletter of the Hubert H. Humphrey Cancer Research Center at Boston University School of Medicine

Sulfur reduction in sediments of marine and evaporite environments

Transformations of sulfur in sediments of ponds ranging in salinities from that of normal seawater to those of brines saturated with sodium chloride were examined. The chemistry of the sediment and pore waters were focused on with emphasis on the fate of sulfate reduction. The effects of increasing salinity on both forms of sulfur and microbial activity were determined. A unique set of chemical profiles and sulfate-reducing activity was found for the sediments of each of the sites examined. The quantity of organic matter in the salt pond sediments was significantly greater than that occurring in the adjacent intertidal site. The total quantitative and qualitative distribution of volatile fatty acids was also greater in the salt ponds. Volatile fatty acids increased with salinity

A spatially-restricted Younger Dryas plateau icefield in the Gaick, Scotland: reconstruction and palaeoclimatic implications

Considerable research has been conducted in Scotland to reconstruct Younger Dryas glaciers and palaeoclimatic conditions, but our understanding remains incomplete. In this contribution, we examine the Gaick, a dissected plateau that extends over ∼520 km 2 in the Central Grampians, Scotland. The extent and style of Younger Dryas glaciation in the Gaick has been repeatedly contested, although a model of extensive plateau icefield glaciation has become generally accepted. This is despite well-documented issues with key elements of the plateau icefield reconstruction. We synthesise the results of recent geomorphological mapping in the Gaick and recognise a distinct morphostratigraphic signature in the upper parts of the western catchments. This differs markedly from sediment-landform associations in other parts of the area, and we argue this provides a strong indication of spatially-restricted Younger Dryas (∼12.9–11.7 ka) glaciation in the Gaick. Our interpretation is independently supported by glacierisation threshold analysis, which implies that the eastern Gaick was unable to nourish Younger Dryas ice. We therefore contest the accepted paradigm of extensive Younger Dryas glaciation in this area. Based on the geomorphological evidence and glacier surface profile modelling, we reconstruct a ∼42 km 2 plateau icefield that yields an equilibrium line altitude of 751 ± 46 m. Using this value, a sea-level precipitation value of 826 ± 331 mm a −1 is inferred for the Younger Dryas, which suggests considerably drier conditions than at present. Using recalculated glacier-derived precipitation estimates from Scotland, we present regional climate analysis that corroborates arguments for a strong west-east precipitation gradient across Scotland

Plasminogen activator in cultured Lewis lung carcinoma cells measured by chromogenic substrate assay.

A chromogenic substrate assay for the plasminogen activator (PA) activity of Lewis lung carcinoma cells has been developed. The cells were incubated with plasminogen, the activation of which to plasmin was measured by the amidolysis of the chromogenic substrate S-2251. This was routinely performed as a 4h serum-free assay, but a variation lasting 24 h, in medium supplemented with plasminogen-free inhibitor-reduced serum, produced similar results. The assay also detected PA released into the medium. PA activity was proportional to cell density, and the assay was non-toxic to the cells. Assays were performed on cultures derived from primary and metastatic tumours. Host cells were effectively eliminated from such cultures but, because of an initial phase of tumour-cell death, PA assays were not carried out until cultures became established. No consistent difference was detected between PA levels in primary and metastatic cultures. However, these cultures were shown to be atypical of the parent tumour; they grew slowly when reinjected at the primary site, and their metastatic potential was impaired