Prélèvement et échantillonnage dans les petits bassins-versants ruraux

Cet article traite de la représentativité des prélèvements d'eau effectués dans le cadre de suivis de bassins versants ou de rivières.Les réflexions proposées sont en premier lieu basées sur des expérimentations de laboratoire et de terrain destinées à comparer divers systèmes et modalités de prélèvements. Les résultats obtenus montrent en particulier que les prélèvements de matières en suspension de dimensions > 100 µm sont peu fiables alors que ce type de particules présente, dans certain cas, une contribution non négligeable aux flux de phosphore total.Les suivis réalisés en divers points représentatifs de petits bassins expérimentaux fournissent des éléments de discussion complémentaires sur le choix des sites des prélèvements et sur les pas de temps de ceux-ci. Nous montrons entre autre, toute l'importance de saisir le début des crues dans les bassins à forte pollution ponctuelle et de considérer des suivis des périodes suffisamment longues pour intégrer les divers fonctionnements hydrochimiques du bassin versant étudié.Water and suspended matter sampling in hydrographic networks is studied through various sampling systems and procedures, and through devices used in fields and in laboratories (fig. 1).Large size particles tend to be underestimated whatever the sampling procedure (manual or automatic) (fig. 2, 3). On the other hand, the sampling for soluble elements and small site particles (less man 50 µm) is very reliable. This is observed in suspended matter concentrations, eg < 2 g.l-1.The largest particles of suspended matter sometimes contribute to nutrient transfer, e.g. phosphorous. This however depends upon the presence of soil microaggregates and nutrient geochemistry. Moreover, natural hydrological variations give rise to another problem for sampling, especially for suspended matter (fig. 7, 9, 10, table 2).Comparatively the variation due to preservation of water is of secondary importance (fig. 11).However, thanks to results from monitoring in experimental watersheds, it is possible to find the best sampling station and the hast time to carry out sampling (fig. 12, 13; fable 3).Duration of survey and intervals between each sample depend, of course, upon the objectives of the study, but also upon watershed characteristics. Considering these characteristics, we propose an example of sampling stategy in the text (table 6, fig. 5)

Bilan du phosphore dans un bassin versant du lac Léman : Conséquences pour la détermination de l'origine des flux exportés

Cet article s'intéresse à la dynamique du transfert du phosphore dans un bassin rural de la région lémanique, le bassin versant du Foron (51,5 km2). Nous montrons qu'en établissant, pour les périodes de tarissement, la relation concentration moyenne en phosphore - débit moyen à l'exutoire, il est possible d'évaluer globalement:1) la rétention du P dans le réseau hydrographique lors de ces périodes; 2) la contribution moyenne des sources ponctuelles de P aux exportations du bassin.La rétention est un phénomène temporaire, mais c'est une étape clé de la dynamique du phosphore. Elle crée d'importants stocks de P dans le réseau hydrographique; ces stocks se situent pour l'essentiel au niveau des sédiments comme l'ont montré diverses études antérieures et sont exportés lors des crues. Les quantités stockées à l'issue de longs étiages dépassent souvent largement les exportations enregistrées pendant les plus fortes crues. La rétention s'accompagne d'une transformation partiellement irréversible de la spéciation du phosphore transféré.Ces phénomènes sont pris en compte pour établir des bilans annuels de pollution, et notamment la balance diffus - ponctuel.The effective management of phosphorus in watersheds requires knowledge of the origin and magnitude of phosphorus inputs. However, for large and complex watersheds, it is impossible to determine the phosphorus mass balances by measuring the flow from each source and the rate of transfer of phosphorus originating from upstream. Because of these difficulties, we have developed a strategy based on the measurement of phosphorus mass balances during base flow periods (with no storm-flow, which means with a constant or decreasing flow). This approach was applied to the Foron River watershed (drainage area 51.5 km2, annual average water discharge 0.5 m3 /s).The Foron River, a hillside stream with a torrential flow regime, is a tributary of Lac Léman (a large meso-eutrophic lake also known as Lake Geneva). Land use within the Foron watershed is diverse, with 50% of the watershed area being in a naturally forested area (on steep upland slopes), 36% in agricultural land lying on a gentle relief of moraine deposits (70% permanent pasture and hay meadows, 30% cereal grain crops) and 3% in marshes. The population is scattered amongst four small villages, with an overall density of about 100 inhabitants per km2. Stream flow in the river was continuously monitored and automatically sampled at the outlet from 1990 to 1993. Additional grab samples were taken at various locations, springs, agricultural sub-watersheds and brooks in the naturally forested area. Total suspended solids, soluble total phosphorus, orthophosphate and total phosphorus were determined according to standard methods. In addition, sampling was carried out to determine phosphorus stored in river sediments in places that are known to be temporary sinks for sediments.To validate the efficiency of the approach, we carried out a detailed study of the point sources and the sewage systems in the watershed. Only two-thirds of domestic wastewaters were processed in treatment plants. The minimum load of point source inputs was established to be about 60 kg total P per week and many point sources (that is to say discharged at discrete locations) remained unidentified (i.e., not all households were connected to sewers, farmsteads contributed for unknown quantities of total P).During base flow periods, phosphorus was predominantly soluble and orthophosphate was found throughout the hydrographic network. Particulate phosphorus and suspended matter concentrations were negligible (respectively 0.8 m3 /s, a constant P export regime was reached as all the inputs were transported to the outlet. Consequently, output at the outlet was equal to input into the river. This approach permits the calculation of the total point sources. Knowing the inputs from point sources and the total P export at the outlet, we can calculate the diffuse sources.Significant relationships between weekly mean total-P, soluble-P or orthophosphate concentrations at the outlet and weekly mean discharge were established to validate these observations. The best fit of these measured values didn't follow a standard law of dilution because the dilution effect was attenuated by the antagonist effect due to the increase in P transport inputs from sewage with increasing discharge. ([soluble P]=0.02+ 0.148 Q-1/2 ; n=82; r2 =0.8).During base flow when Q < 0.8 m3 /s, a portion of phosphorus inputs accumulated in the river biota and sediments through deposition, biological uptake and sorption. This accumulation may explain the higher levels of total-P and the easily exchangeable P in the sediments downstream of point source discharges. The efficiency of these storage processes depends not only on water discharge, but also (secondarily) on some characteristics of the antecedent hydrological conditions. We found that the amount stored increased significantly in the weeks following large storm-flows. We assumed that this indicated the effect of an intense exchange of the P loaded sediments with unsaturated sediments that were generated by erosion of natural upstream areas. As in-stream processes alter phosphorus speciation, a part of soluble-P becomes particulate. During storm-flows following base flow periods with a discharge lower than the limit discharge, the stored phosphorus was released from both particulate and soluble forms and exported. We calculated an annual mass balance of soluble phosphorus to evaluate the amount of particulate phosphorus exported at the outlet due to the transformation of phosphorus from soluble to particulate forms. The result obtained suggested that ¼ of particulate-P (1 t per year) resulted from this speciation change.To summarize, analyses of phosphorus mass balances during base flow could lead to a new procedure to evaluate the mean total mass of P originating from point sources, without referring to field surveys. In addition, this procedure allowed the quantification of both total-P storage as well as changes in speciation that occur during transfer of phosphorus in the hydrographic network

Sound-propagation gap in fluid mixtures

We discuss the behavior of the extended sound modes of a dense binary hard-sphere mixture. In a dense simple hard-sphere fluid the Enskog theory predicts a gap in the sound propagation at large wave vectors. In a binary mixture the gap is only present for low concentrations of one of the two species. At intermediate concentrations sound modes are always propagating. This behavior is not affected by the mass difference of the two species, but it only depends on the packing fractions. The gap is absent when the packing fractions are comparable and the mixture structurally resembles a metallic glass.Comment: Published; withdrawn since ordering in archive gives misleading impression of new publicatio

P53 IMMUNOHISTOCHEMISTRY AS A SURROGATE FOR TP53 MUTATIONAL ANALYSIS IN ENDOMETRIAL CANCER BIOPSIES

Meeting abstract from 17th Biennial Meeting of the International Gynecologic Cancer Society Kyoto, Japan September 14-16, 201

Conservation genomic analysis of domestic and wild pig populations from the Iberian Peninsula

Abstract Background Inbreeding is among the major concerns in management of local livestock populations. The effective population size of these populations tends to be small, which enhances the risk of fitness reduction and extinction. High-density SNP data make it possible to undertake novel approaches in conservation genetics of endangered breeds and wild populations.A total of 97 representative samples of domestic and wild pig populations from the Iberian Peninsula, subjected to different levels of threat with extinction, were genotyped with a 60 K SNP panel. Data analyses based on: (i) allele frequency differences; (ii) linkage disequilibrium and (iii) runs of homozygosity were integrated to study population relationships, inbreeding and demographic history. Results The domestic pigs analyzed belonged to local Spanish and Portuguese breeds: Iberian ─ including the variants Retinto Iberian, Negro Iberian and Manchado de Jabugo ─, Bisaro and Chato Murciano. The population structure and persistence of phase analysis suggested high genetic relations between Iberian variants, with recent crossbreeding of Manchado de Jabugo with other pig populations. Chato Murciano showed a high frequency of long runs of homozygosity indicating recent inbreeding and reflecting the recent bottleneck reported by historical records. The Chato Murciano and the Manchado de Jabugo breeds presented the lowest effective population sizes in accordance with their status of highly inbred breeds. The Iberian wild boar presented a high frequency of short runs of homozygosity indicating past small population size but no signs of recent inbreeding. The Iberian breed showed higher genetic similarities with Iberian wild boar than the other domestic breeds. Conclusions High-density SNP data provided a consistent overview of population structure, demographic history and inbreeding of minority breeds and wild pig populations from the Iberian Peninsula. Despite the very different background of the populations used, we found a good agreement between the different analyses. Our results are also in agreement with historical reports and provide insight in the events that shaped the current genetic variation of pig populations from the Iberian Peninsula. The results exposed will aid to design and implement strategies for the future management of endangered minority pig breeds and wild populations.This project was financially supported by European Research Council under the European Community's Seventh Framework Programme (FP7/2007-2013)/ERC grant #ERC-2009-AdG: 249894 (Sel Sweep project).Peer Reviewe

Teaching child and adolescent psychiatry to undergraduate medical students - A survey in German-speaking countries

Objective To conduct a survey about teaching child and adolescent psychiatry to undergraduate medical students in German-speaking countries. Methods A questionnaire was sent to the 33 academic departments of child and adolescent psychiatry in Germany, Austria, and the German-speaking part of Switzerland. Results All departments responded. For teaching knowledge, the methods most commonly reported were lectures and case presentations. The most important skills to be taught were thought to be how to assess psychopathology in children and how to assess families. For elective courses, the departments reported using a wide range of teaching methods, many with active involvement of the students. An average of 34 hours per semester is currently allocated by the departments for teaching child and adolescent psychiatry to medical students. Required courses are often taught in cooperation with adult psychiatry and pediatrics. Achievement of educational objectives is usually assessed with written exams or multiple-choice tests. Only a minority of the departments test the achievement of skills. Conclusions Two ways of improving education in child and adolescent psychiatry are the introduction of elective courses for students interested in the field and participation of child and adolescent psychiatrists in required courses and in longitudinal courses so as to reach all students. Cooperation within and across medical schools can enable departments of child and adolescent psychiatry, despite limited resources, to become more visible and this specialty to become more attractive to medical students. Compared to the findings in earlier surveys, this survey indicates a trend towards increased involvement of academic departments of child and adolescent psychiatry in training medical students

Threshold Photoelectron Spectrum of Cyclobutadiene: Comparison with Time-Dependent Wavepacket Simulations

The C4H4 isomer cyclobutadiene (CBD) is the prime model for antiaromaticity and thus a molecule of considerable interest in chemistry. Because it is highly reactive, it can only be studied under isolated conditions. Its electronic structure is characterized by a pseudo-Jahn–Teller effect in the neutral and a E ⊗ β Jahn–Teller effect in the cation. As a result, recording photoelectron spectra as well as describing them theoretically has been challenging. Here we present the photoion mass-selected threshold photoelectron spectrum of cyclobutadiene together with a simulation based on time-dependent wavepacket dynamics that includes vibronic coupling in the ion, taking into account eight vibrational modes in the cation. Excellent agreement between theory and experiment is found, and the ionization energy is revised to 8.06 ± 0.02 eV