TR-644 a novel potent tubulin binding agent induces impairment of endothelial cells function and inhibits angiogenesis.

TR-644 is a novel combretastatin A-4 (CA-4) analogue endowed with potent microtubule depolymerizing activity superior to that of the lead compound and it also has high affinity to colchicines binding site of tubulin. We tested TR-644 anti-angiogenic effects in human umbilical endothelial cells (HUVEC). It showed no significant effects on the growth of HUVEC cells at concentrations below 1,000 nM, but at much lower concentrations (10-100 nM) it induced inhibition of capillary tube formation, inhibition of endothelial cell migration and affected endothelial cell morphology as demonstrated by the disruption of the microtubule network. TR-644 also increased permeability of HUVEC cells in a time dependent manner. The molecular mechanism for the anti-vascular activity of TR-644 was investigated in detail. TR-644 caused G2/M arrest in endothelial cells and this effect correlated with downregulation of the expression of Cdc25C and Cdc2Tyr15. Moreover TR-644 inhibited VEGF-induced phosphorylation of VE-cadherin but did not prevent the VEGF-induced phosphorylation of FAK. In chick chorioallantoic membrane in vivo assay, TR-644 (0.1-1.0 pmol/egg) efficiently counteracted the strong angiogenic response induced by FGF. Also CA-4, used as reference compound, caused an antagonistic effect, but in contrast, it induced per se, a remarkable angiogenic response probably due to an inflammatory reaction in the site of treatment. In a mice allogenic tumor model, immunohistochemical staining of tumors with anti-CD31 antibody showed that TR-644 significantly reduced the number of vessel, after 24 h from the administration of a single dose (30 mg/Kg)

Identification of the muscular proteins of pigs submitted to the eletroforesis in polyacrilamide gel with Sodium Dodecyl Sulfate (SDS)

Fragments of two pigs muscles of Landrace, Large White, Duroc purebred and crossbred, the eletroforesis was submitted in polyacrilamide gel with SDS, being objectified to verify there would be a specific pattern for pigs and to identify through the molecular weights, the main proteins. The results show that there would be a specific pattern for swine and they were identified miosina heavy chain, actina, troponina, tropomiosina and a light chain of miosina

Variabilidade genética de duas populações de Astyanax altiparanae da bacia do alto rio Paraná.

O presente trabalho tem como objetivo caracterizar a estrutura genética de duas populações de A. altiparanae da bacia do alto rio Paraná (Paranapanema e Tietê) através da análise de 11 marcadores moleculares do tipo microssatélite.Organizado por: Sílvio Ricardo Maurano; AQUACIÊNCIA 2012

Characterization of genetic-biochemical polymorphism of enzymes in brazilian Mangalarga mares

In this tudy the polymorphism of transferrins was determined using polyacrylamide gel electrophoresis, in a group of Brazilian Mangalarga mares. Blood plasma samples of 45 mares in reproductive age were used. The existence of patterns of 2, 3 and 4 bands with different mobilities, and of nine transferrin genotypes was demonstrated. Genotypes DD (22.2%) and DF (26.6%) were the most frequent, which is compatible with the situaiton found in the breeds of origin (Adalusian, Thoroughbred and Arabian) of the Mangalarga horse. The allele FfD was the most frequent, and could represent a possible environmental adaptation or a characteristic of sadle horses

Genetic-biochemical polymorphism of enzymes in Brazilian Mangalarga mares

A study was conducted with a group of mares of the Mangalarga breed in Brazil, with the aim of determining the electrophoretic pattern, in polyacrylmide gel, of the enzymes Acid Phosphatase, Alkaline Phosphatase and Peroxidase. Blood plasma samples of 45 mares in reproductive age were used. For Acid Phosphatase, we observed the existence of two alleles, A and B, with allelic frequencies of 0.28 and 0.72, respectively: corresponding to three genotypes AA, AB and BB. Allelic frequency demonstrated that the group was in Hardy-Weinberg-Castle balance. For Alkaline Phosphatase, three genotypes were verified: Type I (HA1), presenting only one band of fast migration; Type II (HA1-HA2 and HA1-HA3), presenting two bands, with HA2 of intermediary position and HA3 of slower motility; and Type III, presenting all three bands HA1, HA2 and HA3. Type I was most frequent, occurring in 21 animals. For Peroxidase, all animals presented a pattern of two bands, designated as genotype I, suggesting that this is a monomorphic locus in the population under study