Comparison of biomechanical changes in respect of pain scores after lumbar fusion

Low back pain is one of the leading causes of disability, some patients don’t respond to conservative therapy, and must undergo surgery. The aim of our study was to investigate the results of lumbar fusion operations, especially considering the changes in the sagittal range of motion (ROM) in the adjacent segments, thoracic, lumbar region and pain. 13 patients were involved, who had lumbar LIV/V rigid fusion (TLIF) operation, and still had low back pain symptoms. For the biomechanical measurement we used the Spinal Mouse, a computer associated device, based on electromagnetic impulses. For estimating the pain we used the 3D pain questionnaire (West-Haven-Yale Multidimensonal Pain Inventory [WHYMPI]).In the outline analysis we found that the decrease in pain and improvement of symptoms after a lumbar fusion is defi nitely the result of increased thoracic segment hypermobility, decreased lumbar segment hypomobility, decreased proximal adjacent segment hypomobility and, increase of the distal adjacent segment hypomobility. The sagittal range of motion (ROM) of the whole spine, as the hypomobility is corrected towards the normal ROM resulted in decrease of pain. In conclusion we can notice when using semirigid systems for bridging adjacent segments, it is important to secure the hypermobility of the thoracic spine, and the mobility of the proximal segment. DOI: 10.17489/biohun/2010/1/0

PRELIMINARY STRUCTURAL OPTIMIZATION OF SOME FUMONISIN METABOLITES BY DENSITY FUNCTIONAL THEORY CALCULATION

Maize (Zea mays L.) is often contaminated with Fusarium verticillioides. This harmful fungus produces fumonisins as secondary metabolites. These fumonisins can appear both free and hidden form in planta. The hidden form is usually bound covalently to cereal starch. From the hidden fumonisins, during enzymatic degradation, glycosides are formed, and the fumonisin is further decomposed during a de-esterification step. In this short communication some preliminary DFT calculated structural results which could be useful in the future to help to understand the van der Waals force controlled molecular interactions between these kinds of mycotoxin molecules and enzymes are demonstrated

Correspondence of biomechanical changes in respect of pain score and psychological status after the standard and enhanced spine stabilisation trainings

Low back pain is one of the leading causes of disability. Exercise therapy is a management strategy that is widely used in low back pain. The aim of our study was to investigate the results of standard and enhanced exercise therapy. 15 patients who had low back pain were involved in the study, none of the patients had had spinal operations before. In the fi rst group the patients did the exercises every day at home. The patients of the second group did the exercises 3 times a week. For the biomechanical measurement we used the Spinal Mouse, a computer associated device, based on electromagnetic impulses. For estimating pain we used the 3D pain questionnaire (West-Haven-Yale Multidimensional Pain Inventory [WHYMPI]).In the outline analysis there was a signifi cant rise in the capacity (

ASSESSING THE POSSIBLE INTERACTION BETWEEN CARDUUS MARIANUS AND DIETARY DEOXYNIVALENOL ON CAECAL MICROBIOTA AND FERMENTATION OF GROWING RABBITS

Contamination of feed with mycotoxins is a common problem encountered in animal farming. Mycotoxin exposure can affect adversely the health of animals. In rabbits caecal fermentation is an essential digestive process being indication of physiological alterations. Deoxynivalenol (DON) is one of the most frequent contaminants of grains which affect the growth of monogastric animals. Data about dietary DON and its effect in rabbits are scarce. Medicinal plants are often used as feed additives to enhance the performance of the animals. Carduus marianus (milk thistle) is known for its hepatoprotective and antioxidant effects (among others) but no data are available about the effect on rabbit caecum. Considering the aforementioned, the aim of this study was to assess the possible interactive effect of Carduus marianus and DON on the caecum of growing rabbits. 75 Pannon White rabbits were reared for six weeks from 35 (after weaning) till 77 days of age. Rabbits received the following diets: control (C), control with DON (CT), control supplemented with C. marianus in 0,5% (H1), control supplemented with C. marianus in 0,5% and DON (H1T), control supplemented with C. marianus in 1% (H2) and control supplemented with C. marianus in 1% and DON (H2T). On slaughter, caecum was collected for the analysis of total volatile fatty acids (VFA) and the microbiota of the caecum, pH of the caecum was also recorded. There was no significant difference in total VFA concentration or individual VFA. Number of aerobic bacteria significantly differed among toxin and non-toxin groups. DON affected adversely the number of aerobic bacteria. An interactive effect of DON and Carduus marianus on E.coli number was observed. There was no effect on total or individual VFA amounts

In vitro Interaction between Fumonisin B1 and the Intestinal Microflora of Pigs

The caecal chyme of pigs was incubated anaerobically in McDougall buffer with and without fumonisin B1 (5 μg/ml) for 0, 24 and 48 h. The plate count agar technique was applied for enumerating the amount of bacteria including aerobic, anaerobic bacteria, coliform, Escherichia coli and Lactobacillus sp. The quantitative polymerase chain reaction was also performed to estimate the number of copies of the total bacteria, Lactobacillus, Bacteroides and Prevotella. No significant differences in the amount of bacterial groups between the experimental (buffer, chyme, and fumonisin B1) and control 1 groups (buffer + chyme) were observed in both methods. Fumonisin B1 and hydrolysed fumonisin B1 concentration were analysed by liquid chromatograghy – mass spectrometry. There was no significant difference in FB1 concentration between the experimental and the control 2 group (buffer and fumonisin B1) at 0 h incubation, 5.185 ± 0.174 μg/ml com­pared with 6.433 ± 0.076 μg/ml. Fumonisin B1 concentration in the experimental group was reduced to 4.080 ± 0.065 μg/ml at 24 h and to 2.747 ± 0.548 μg/ml at 48 h incubation and was significantly less than that of in the control group. Hydrolysed fumonisin B1 was detected after 24 h incubation (0.012 ± 0 μg/ml). At 48 h incubation time, hydrolysed fumonisin B1 concentration was doubled to 0.024 ± 0.004 μg/ml. These results indicate that fumonisin B1 can be metabolised by caecal microbiota in pigs though the number of studied bacteria did not change

Subchronic exposure to deoxynivalenol exerts slight effect on the immune system and liver morphology of growing rabbits

As the most common grain contaminant worldwide, deoxynivalenol is of high importance despite its low toxicity compared to other trichothecene mycotoxins. Data on the effects of deoxynivalenol in rabbits are scarce. Thus, the aim of this study was to investigate the effects of dietary deoxynivalenol fed at a high level (10 mg/kg of feed) on the productive performance, blood indices, immunological variables, histopathological changes, and genotoxicity in rabbits. Forty-eight Pannon White rabbits were exposed to contaminated diets for three weeks. Despite its high concentration, deoxynivalenol did not affect the feed intake, body weight, and body weight gain. Liver and kidney function was not affected, as shown by the clinical chemistry indices. Conversely, in two rabbits the toxin caused mild fibrosis of the liver, without degenerative changes of the hepatocytes. No genotoxicity could be observed either. Gut cytokines and the phagocytic activity of the macrophages did not differ significantly. The percentage of neutrophils was significantly lower, whereas that of eosinophils was significantly higher in the toxin-fed group. Deoxynivalenol did not cause significant changes in gut and villus morphology. In 4 out of the 6 deoxynivalenol-treated animals, the ratio of lymphoblast proliferation and simultaneous apoptosis shifted towards apoptosis in the gut-associated lymphoid tissue. In the central part of the lymphoid follicles of the spleen, lymphocyte depletion and follicular atrophy could be detected. It can be concluded that rabbits are less sensitive to deoxynivalenol, but the findings confirm that this Fusarium toxin is capable of modulating the immune response

Az új generációs szekvenálás jelentősége az akut mieloid leukémia precíziós onkológiai megközelítésében

A szolid tumorokkal összehasonlítva az akut mieloid leukémia (AML) hátterében a gyerekkori daganatokhoz hasonló alacsony számú genetikai eltérés, átlagosan 3−5 szomatikus mutáció áll. Bár a mutációs háttér igen heterogén, a genetikai eltérések kimutatása diagnosztikai, prognosztikai és terápiás jelentőséggel bír. Jelen tanulmányunk 2001–2019 között intézetünkben diagnosztizált 830 AML-es beteg citogenetikai, valamint a leggyakrabban előforduló mutációs eltéréseit és azok társulásait vizsgálja. A laboratóriumban nemrégiben bevezetett új generációs szekvenálás (NGS) eredményei hét beteg esetében szintén bemutatásra kerülnek. A korábban más technikával vizsgált eltérések célzott vizsgálata és az NGS megegyező eredményt hozott. Az NGS technikával azonosíthatóak mindazok a további, ritkábban előforduló genetikai eltérések, amelyek az AML diagnosztikai és prognosztikai besorolását tovább finomítják az Európai LeukémiaNet ajánlásai szerint. Az NGS technika alkalmazása a nemzetközi tapasztalatokat követően hazánkban is a rutin diagnosztikai vizsgálómódszerek közé kell, hogy beemelkedjen. Magy Onkol 63:282-287, 201