Nucleation dynamics in 2d cylindrical Ising models and chemotaxis

The aim of our work is to study the effect of geometry variation on nucleation times and to address its role in the context of eukaryotic chemotaxis (i.e. the process which allows cells to identify and follow a gradient of chemical attractant). As a first step in this direction we study the nucleation dynamics of the 2d Ising model defined on a cylindrical lattice whose radius changes as a function of time. Geometry variation is obtained by changing the relative value of the couplings between spins in the compactified (vertical) direction with respect to the horizontal one. This allows us to keep the lattice size unchanged and study in a single simulation the values of the compactification radius which change in time. We show, both with theoretical arguments and numerical simulations that squeezing the geometry allows the system to speed up nucleation times even in presence of a very small energy gap between the stable and the metastable states. We then address the implications of our analysis for directional chemotaxis. The initial steps of chemotaxis can be modelled as a nucleation process occurring on the cell membrane as a consequence of the external chemical gradient (which plays the role of energy gap between the stable and metastable phases). In nature most of the cells modify their geometry by extending quasi-onedimensional protrusions (filopodia) so as to enhance their sensitivity to chemoattractant. Our results show that this geometry variation has indeed the effect of greatly decreasing the timescale of the nucleation process even in presence of very small amounts of chemoattractants.Comment: 27 pages, 6 figures and 2 table

Neutrophils amplify the formation of DNA adducts by benzo[a]pyrene in lung target cells.

Inflammatory cells and their reactive oxygen metabolites can cause mutagenic effects in lung cells. The purpose of this study was to investigate the ability of activated neutrophils to modulate DNA binding of benzo[a]pyrene (B[a]P), a known carcinogen, in lung target cells. Equivalent numbers of rat lung epithelial cells (RLE-6TN cell line) and freshly isolated human blood neutrophils (PMN) were coincubated in vitro for 2 hr after addition of benzo[a]pyrene (0.5 microM) or two of its trans-diol metabolites, with or without stimulation with phorbol myristate acetate (PMA). DNA adducts of B[a]P-metabolites were determined in target cells using 32P-postlabeling; oxidative DNA damage (7-hydro-8-oxo-2'-deoxyguanosine [8-oxodG]) was evaluated by high performance liquid chromatography with electrochemical detection. Increased DNA adducts were observed in lung cells coincubated with polymorphonuclear leukocytes (PMN). Activation of PMN with PMA, or addition of more activated PMN in relation to the number of lung cells, further increased the number of adducts, the latter in a dose-response manner. Incubation with B[a]P-4,5-diol did not result in any adduct formation, while B[a]P-7,8-diol led to a significant number of adducts. Moreover, PMA-activated PMN strongly enhanced adduct formation by B[a]P-7,8-diol, but not 8-oxodG, in lung cells. The addition of antioxidants to the coincubations significantly reduced the number of adducts. Results suggest that an inflammatory response in the lung may increase the biologically effective dose of polycyclic aromatic hydrocarbons (PAHs), and may be relevant to data interpretation and risk assessment of PAH-containing particulates

X-ray phase-contrast imaging for laser-induced shock waves

X-ray phase-contrast imaging (XPCI) is a versatile technique with applications in many fields, including fundamental physics, biology and medicine. Where X-ray absorption radiography requires high density ratios for effective imaging, the image contrast for XPCI is a function of the density gradient. In this letter, we apply XPCI to the study of laser-driven shock waves. Our experiment was conducted at the Petawatt High-Energy Laser for Heavy Ion EXperiments (PHELIX) at GSI. Two laser beams were used: one to launch a shock wave and the other to generate an X-ray source for phase-contrast imaging. Our results suggest that this technique is suitable for the study of warm dense matter (WDM), inertial confinement fusion (ICF) and laboratory astrophysics

Propagation-based imaging phase-contrast enhanced imaging setup for single shot acquisition using laser-generated X-ray sources

The development of new diagnostics is important to improve the interpretation of experiments. Often well-known physical processes and techniques originally developed in unrelated fields of science can be applied to a different area with a significant impact on the quality of the produced data. X-ray phase-contrast imaging (XPCI) is one techniques which has found many applications in biology and medicine. This is due to its capability to emphasise the presence of strong density variations normally oriented with respect to the X-ray propagation direction. With the availability of short energetic X-ray pulses XPCI extends to time-resolved pump-probe measurements of laser-matter interaction where strong density gradient are also present. In this work we present the setup for XPCI tested at the laser PHELiX at GSI in Germany

Nano-technology and nano-toxicology

Rapid developments in nano-technology are likely to confer significant benefits on mankind. But, as with perhaps all new technologies, these benefits are likely to be accompanied by risks, perhaps by new risks. Nano-toxicology is developing in parallel with nano-technology and seeks to define the hazards and risks associated with nano-materials: only when risks have been identified they can be controlled. This article discusses the reasons for concern about the potential effects on health of exposure to nano-materials and relates these to the evidence of the effects on health of the ambient aerosol. A number of hypotheses are proposed and the dangers of adopting unsubstantiated hypotheses are stressed. Nano-toxicology presents many challenges and will need substantial financial support if it is to develop at a rate sufficient to cope with developments in nano-technology