In Vitro Propagation Of Sacha Inchi [propagação In Vitro De Sacha Inchi]

The aim of this study was to perform an in vitro evaluation of the auxin:cytokinine ratio in different segments of the epicotyl and hypocotyl of Sacha inchi (Plukenetia Volubilis Linneo) seeds germinated in vitro. The segments apical (A), median (B) and basal (C) were introduced into semi-solid MS culture medium (2.0g L -1 Phytagel), supplemented with MS vitamins, sucrose (30.0g L -1) and submitted to three doses of auxin indolebutyric acid - IBA (0; 0.1; 0.5mg L -1), associated with four doses of the cytokinine benzylaminopurine - BAP (0; 0.1; 0.5; 1.0mg L -1), totaling 36 treatments. After nine weeks of in vitro cultivation, the apical segment (A) presented shoot formation by direct organogenesis at the concentrations of 0.5 and 1.0 of BAP associated with 0.0 and 0.1 of IBA. It is feasible to use in vitro cultivation with the apical region of seeds germinated in vitro used as explants.42711681172Céspedes, E.I.M., (2006) Cultivo de Sacha Inchi, p. 11. , Tarapoto, San Martin, Peru: INIIA, Subdirección De Recursos Geneticos Y BiotecnologíaDimech, A.M., Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia (2007) Plant Cell, Tissue and Organ Culture, 88, pp. 157-165. , http://www.springerlink.com/content/k070lrjll72x370g/fulltext.html, 9188. Disponível em:,. Acesso em: 02 fev. 2007. doi: 10.1007/s11240-006--1Guillén, M.D., Characterization of Sacha Inchi (Plukenetia volubilis L.) Oil by FTIR Spectroscopy and 1H NMR. Comparison with Linseed Oil (2003) Journal of the American Oil Chemist Society, 80, pp. 755-762Huamán, J., Efecto de la Plukenetia volubilis Linneo (Sacha inchi) en la trigliceridemia posprandial (2008) Annales Faculdad Medicina, 69, pp. 263-266Jesus, A.M.S., Cultivo in vitro de embriões zigóticos de Jatropha (2003) Revista Ceres, 50, pp. 183-189Lima, G.P.P., Efeito do BAP e ANA e atividade da peroxidase em mandioca (Manihot esculenta Crantz cv Mcol 22) cultivada in vitro (2002) Revista Brasileira de Agrociência, 8 (2), pp. 127-130Mala, J., Role of phytohormones in organogenic ability of elm multiplicated shoots (2005) Biologia Plantarum, 50, pp. 8-14Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497Nunes, C.F., Diferentes suplementos no cultivo in vitro de embriões de pinhão manso (2008) Pesquisa agropecuária brasileira, 43 (1), pp. 9-14Vazquez Molina, D.E., Sugar cane buds as an efficient explant for plantlet regeneration (2005) Biologia Plantarum, 49, pp. 481-485. , http://springerlink.com/content/l165h14730u55466/, 2005. Disponível em:,. Acesso em: 03 se

Horticultural Performance Of In Vitro Propagated Plants Of Sacha Inchi [desempenho Horticultural De Plantas Propagadas In Vitro De Sacha Inchi]

The aim of this study was to evaluate, in the field, the horticultural characteristics of Sacha inchi (Plukenetia volubilis L.) in vitro plants considering time of flowering and harvesting, the productivity, as well as their morphological characteristics: color and shape of leaves, stems and fruits. The plantlets, originated from the apex of in vitro plants, were propagated in culture medium with MS salts and vitamins, add 30g L-1 sucrose and 1.0mg L-1 BAP for six weeks. Were selected at random, four plants propagated in vitro, four plants by seminal and twenty-two plants, also by composing the border, totaling thirty plants. Analyses were completed weekly until fourteen months after planting, which occurred before the first pruning. The results indicate the non-occurrence of genetic variability in vitro grown plants, as well as early in the production and increased productivity as compared with the conventional material obtained via seminal.44610501053Bordignon, S.R., Propagação in vitro de Sacha inchi (Plukenetia volubilis Linneo) (2012) Ciência Rural, 42 (7), pp. 1168-1172. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103_84782012000700005&lng=en&nrm=iso, cesso em: 05 Feb. 2014. Doi: 10.1590/S0103-84782012005000049Céspedes, E.I.M., (2006) Cultivo De Sacha Inchi, p. 11. , Tarapoto, San Martin, Peru: INIIA, Subdirección De Recursos Geneticos Y BiotecnologíaGuillén, M.D., Characterization of Sacha Inchi (Plukenetia volubilis L.) oil by FTIR spectroscopy and 1H NMR. Comparison with linseed oil (2003) Journal of the American Oil Chemist Society, 80, pp. 755-762. , http://link.springer.com/article/10.1007%2Fs11746-003-0768-z#, Acesso em: 06 Feb. 2014. Doi: 10.1007/s11746-003-0768-zHuamán, J., Efecto de la Plukenetia volubilis Linneo (Sacha inchi) en la trigliceridemia posprandial (2008) Annales Faculdad Medicina, 69, pp. 263-266Murashige, T., Skoog, F., A revised medium for rapid growth and bioassays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497Rodrigues, P.H.V., Heliconia bihai var. Lobster Claw I: Cut flower field production from micropropagated- versus rhizome-derived plants (2006) Floriculture, Ornamental and Plant Biotechnology: Advances and Topical Issues, 2, pp. 558-560. , SILVA da, J.A.T. (Org, Londres: Global Science BooksRodrigues, P.H.V., Variação somaclonal em mudas micropropagadas de abacaxi ornamental, Ananas Bracteatus Schultes var. Striatus (2007) Revista Brasileira De Horticultura Ornamental, 12 (2), pp. 122-125. , http://132.248.9.34/hevila/Revistabrasileiradehorticulturaornamental/2006/vol12/no2/8.pdf, Acesso em: 06 Feb. 2014Rodrigues, P.H.V., Somaclonal variation in micropropagated Heliconia bihai cv. Lobster Claw I Plantlets (Heliconiaceae) (2008) Scientia Agricola, 65, pp. 681-684. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103_90162008000600017&lng=en&nrm=iso, Acesso em: 06 Feb. 2014. Doi: 10.1590/S0103-90162008000600017Santos, C.C.C., Rodrigues, P.H.V., Ocorrência de variação somaclonal em mudas de bananeira micropropagadas da cultivar Pacovan (Musa spp., grupo AAB) (2004) Revista Bragantia, 63 (2), pp. 201-205. , http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006_87052004000200005&lng=en&nrm=iso, Acesso em: 06 Feb. 2014. Doi: 10.1590/S0006-8705200400020000

In Vitro Culture Of Sugarcane Exposed To Different Light Sources [cultivo In Vitro De Cana-de-açúcar Exposta A Diferentes Fontes De Luz]

The objective of this work was to evaluate the effect of different wave length on the in vitro development of sugarcane seedlings. Explants were subjected to four light emitting diodes (LED): 100% blue; 70% blue + 30% red; 30% blue + 70% red; 100% red, besides the control with white fluorescent lamp. Seedlings were evaluated for: number of shoots; height; fresh and dry matter mass; and contents of carotenoids and chlorophyll a and b. It was observed thylakoid disarrangement in the chloroplast, proportional to the increasing incidence of red light. The size of the in vitro produced seedlings reduces with the increase of red light incidence.48913031307Barros, A.C.B., Costa, D.A., Medeiros, E.C., Biorreator de imersão temporária aplicado na biofábrica de cana-de-açúcar. In: GERALD, L.T.S Biofábrica de plantas, pp. 2011-71. , Ed produção industrial de plantas in vitro. São Paulo: AntiquaDekker, J.P., Boekema, E.J., Supramolecular organization of thylakoid membrane proteins in green plants (2005) Biochimica et Biophysica Acta - Bioenergetics, 1706, pp. 12-39. , DOI:10.1016/j.bbabio.2004.09.009Gratão, P.L., Monteiro, C.C., Rossi, M.L., Martinelli, A.P., Peres, L.E.P., Medici, L.O., Lea, P.J., Azevedo, R.A., Differential ultrastructural changes in tomato hormonal mutants exposed to cadmium (2009) Environmental and Experimental Botany, 67, pp. 387-394. , DOI:10.1016/j.envexpbot.2009.06.017Huan, L.V.T., Tanaka, M., Effects of red and blue light-emitting diodes on callus induction, callus proliferation, and protocorm-like body formation from callus in Cymbidium orchid (2004) Environment Control in Biology, 42, pp. 57-64. , DOI:10.2525/ecb1963.42.57Kim, S.J., Hahn, E.J., Heo, J.W., Peak, K.Y., Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro (2004) Scientia Horticulturae, 101, pp. 143-151. , DOI:10.1016/j.scienta.2003.10.003Kiss, A.Z., Ruban, A.V., Horton, P., The PsbS protein controls the organization of the photosystem II antenna in higher plant thylakoid membranes (2009) The Journal of Biological Chemistry, 283, pp. 3972-3978. , DOI:10.1074/jbc.M707410200Lichtenthaler, H.K., Chlorophylls and carotenoids: pigments of photosynthetic biomembranes (1987) Methods in Enzymology, 148, pp. 350-382. , DOI:10.1016/0076-6879(87)48036-1Murashige, T., Skoog, F., A revised medium for rapid growth and bio assays with tobacco tissue cultures (1962) Physiologia Plantarum, 15, pp. 473-497. , DOI:10.1111/j.1399-3054.1962.tb08052.xNhut, D.T., Takamura, T., Watanabe, H., Okamoto, K., Tanaka, M., Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell (2003) Tissue and Organ Culture, 73, pp. 43-52. , DOI:10.1023/A:1022638508007Rocha, P.S.G., da Oliveira, R.P., de Scivittaro, W.B., Santos, U.L., dos. Diodos emissores de luz econcentrações de BAP na multiplicação in vitro de morangueiro (2010) Ciência Rural, 40, pp. 1922-1928. , DOI:10.1590/S0103-84782010000900011Saebø, A., Krekling, T., Appelgren, M., Light quality affects photosynthesis and leaf anatomy of birch plantlets in vitro (1995) Plant Cell, Tissue and Organ Culture, 41, pp. 177-185. , DOI:10.1007/BF00051588Teixeira, J.B., (2006) Produção de mudas clonais em biofábricas e uso de biorreator, p. 27. , Brasília: Embrapa Recursos Genéticos e Biotecnologia (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 180)Veiga, C.F.M., (2006) Diagnóstico da cadeia produtiva da cana-de-açúcar do Estado do Rio de Janeiro, p. 107. , Rio de Janeiro Faerj: Sebra

Sulfasalazine prevents T-helper 1 immune response by suppressing interleukin-12 production in macrophages

Interleukin-12 (IL-12) plays a pivotal role in the development of T-helper 1 (Th1) immune response, which may be involved in the pathogenesis of chronic inflammatory autoimmune disorders. In this study we investigated the effects of sulfasalazine, a drug for treating inflammatory bowel disease and rheumatoid arthritis, on the production of IL-12 from mouse macrophages stimulated with lipopolysaccharide (LPS). Sulfasalazine potently inhibited the production of IL-12 in a dose-dependent manner, in part through the down-regulation of nuclear factor κB (NFκB) activation in IL-12 p40 gene. Activation of macrophages by LPS resulted in markedly enhanced binding activities to the κB site, which significantly decreased upon addition of sulfasalazine as demonstrated by an electrophoretic gel shift assay. Importantly, macrophages pretreated with sulfasalazine either in vitro or in vivo reduced their ability to induce interferon-γ (IFN-γ) and increased the ability to induce IL-4 in antigen-primed CD4+ T cells. From these results, sulfasalazine may induce the Th2 cytokine profile in CD4+ T cells by suppressing IL-12 production in macrophages, and sulfasalazine-induced inhibition of IL-12 production in macrophages may explain some of the known biological effects of sulfasalazine